Molecular characterization of extraintestinal and diarrheagenic Escherichia coli blood isolates
Pathogenic E. coli strains can be classified into two major groups, based on the presence of specific virulence factors: extraintestinal pathogenic E. coli (ExPEC) and diarrheagenic E. coli (DEC). Several case reports describe that DEC can cause bloodstream infections in some rare cases. This mainly...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2022.2147735 |
| _version_ | 1828096775341211648 |
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| author | Vanstokstraeten R. Crombé F. Piérard D. Castillo Moral A. Wybo I. De Geyter D. Janssen T. Caljon B. Demuyser T. |
| author_facet | Vanstokstraeten R. Crombé F. Piérard D. Castillo Moral A. Wybo I. De Geyter D. Janssen T. Caljon B. Demuyser T. |
| author_sort | Vanstokstraeten R. |
| collection | DOAJ |
| description | Pathogenic E. coli strains can be classified into two major groups, based on the presence of specific virulence factors: extraintestinal pathogenic E. coli (ExPEC) and diarrheagenic E. coli (DEC). Several case reports describe that DEC can cause bloodstream infections in some rare cases. This mainly concerns a few specific sequence types that express virulence factors from both ExPEC and DEC. In this study, we retrospectively analysed 234 E. coli blood isolates with whole genome sequencing (WGS). WGS was performed on an Illumina NovaSeq6000. Genotyping was performed using BioNumerics software. The presence of genes was determined with a minimum percentage sequence identity (ID) threshold of 95% and a minimum length for sequence coverage of 95%. Three of the 234 (1.28%) isolates were defined as DEC, 182 (77.78%) as ExPEC, and 49 (20.94%) did not carry pathotype-associated virulence genes. We identified 112 different virulence genes, 48 O-antigens, and 28 H-antigens 82 STs, among the 234 analyzed isolates. ST131 and ST88 were related to healthcare-associated infections. This study provides insight into the prevalence of virulence factors in a large set of E. coli blood isolates from the UZ Brussel. It illustrates high diversity in virulence profiles and highlights the potential of DEC to carry virulence factors associated with extraintestinal infections, making it possible for unusual pathotypes to invade and survive in the bloodstream causing bacteraemia. Diarrheagenic strains causing bacteremia are rare and presently underreported, but modern sequencing techniques will better underscore their importance. |
| first_indexed | 2024-04-11T07:35:41Z |
| format | Article |
| id | doaj.art-dc2ccc8c6ce3482cbd09c38bfdcbdce9 |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-04-11T07:35:41Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-dc2ccc8c6ce3482cbd09c38bfdcbdce92022-12-22T04:36:44ZengTaylor & Francis GroupVirulence2150-55942150-56082022-12-011312032204110.1080/21505594.2022.2147735Molecular characterization of extraintestinal and diarrheagenic Escherichia coli blood isolatesVanstokstraeten R.0Crombé F.1Piérard D.2Castillo Moral A.3Wybo I.4De Geyter D.5Janssen T.6Caljon B.7Demuyser T.8Department of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis, Brussels, BelgiumDepartment of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis, Brussels, BelgiumDepartment of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis, Brussels, BelgiumDepartment of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis, Brussels, BelgiumDepartment of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis, Brussels, BelgiumDepartment of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis, Brussels, BelgiumVrije Universiteit Brussel (VUB), Universitair Ziekenhuis, Brussels Interuniversity Genomics High Throughput core (BRIGHTcore) platform, Brussels, BelgiumVrije Universiteit Brussel (VUB), Universitair Ziekenhuis, Brussels Interuniversity Genomics High Throughput core (BRIGHTcore) platform, Brussels, BelgiumDepartment of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis, Brussels, BelgiumPathogenic E. coli strains can be classified into two major groups, based on the presence of specific virulence factors: extraintestinal pathogenic E. coli (ExPEC) and diarrheagenic E. coli (DEC). Several case reports describe that DEC can cause bloodstream infections in some rare cases. This mainly concerns a few specific sequence types that express virulence factors from both ExPEC and DEC. In this study, we retrospectively analysed 234 E. coli blood isolates with whole genome sequencing (WGS). WGS was performed on an Illumina NovaSeq6000. Genotyping was performed using BioNumerics software. The presence of genes was determined with a minimum percentage sequence identity (ID) threshold of 95% and a minimum length for sequence coverage of 95%. Three of the 234 (1.28%) isolates were defined as DEC, 182 (77.78%) as ExPEC, and 49 (20.94%) did not carry pathotype-associated virulence genes. We identified 112 different virulence genes, 48 O-antigens, and 28 H-antigens 82 STs, among the 234 analyzed isolates. ST131 and ST88 were related to healthcare-associated infections. This study provides insight into the prevalence of virulence factors in a large set of E. coli blood isolates from the UZ Brussel. It illustrates high diversity in virulence profiles and highlights the potential of DEC to carry virulence factors associated with extraintestinal infections, making it possible for unusual pathotypes to invade and survive in the bloodstream causing bacteraemia. Diarrheagenic strains causing bacteremia are rare and presently underreported, but modern sequencing techniques will better underscore their importance.https://www.tandfonline.com/doi/10.1080/21505594.2022.2147735Escherichia colivirulence factorspathotypessequence typeserotypenosocomial |
| spellingShingle | Vanstokstraeten R. Crombé F. Piérard D. Castillo Moral A. Wybo I. De Geyter D. Janssen T. Caljon B. Demuyser T. Molecular characterization of extraintestinal and diarrheagenic Escherichia coli blood isolates Virulence Escherichia coli virulence factors pathotypes sequence type serotype nosocomial |
| title | Molecular characterization of extraintestinal and diarrheagenic Escherichia coli blood isolates |
| title_full | Molecular characterization of extraintestinal and diarrheagenic Escherichia coli blood isolates |
| title_fullStr | Molecular characterization of extraintestinal and diarrheagenic Escherichia coli blood isolates |
| title_full_unstemmed | Molecular characterization of extraintestinal and diarrheagenic Escherichia coli blood isolates |
| title_short | Molecular characterization of extraintestinal and diarrheagenic Escherichia coli blood isolates |
| title_sort | molecular characterization of extraintestinal and diarrheagenic escherichia coli blood isolates |
| topic | Escherichia coli virulence factors pathotypes sequence type serotype nosocomial |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2022.2147735 |
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