Favorable osteogenic activity of iron doped in silicocarnotite bioceramic: In vitro and in vivo Studies
Background: Calcium phosphate silicate (Ca5(PO4)2SiO4 or CPS) is a promising bioceramic for bone grafting. Iron (Fe) is a trace element in the human body that has been reported to enhance the mechanical strength of CPS ceramics. However, the exact biofunctions of Fe, combined with another human trac...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Journal of Orthopaedic Translation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214031X21001054 |
| _version_ | 1819176561077649408 |
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| author | Jingwei Zhang Fanyan Deng Xiaoliang Liu Yuwei Ge Yiming Zeng Zanjing Zhai Congqin Ning Huiwu Li |
| author_facet | Jingwei Zhang Fanyan Deng Xiaoliang Liu Yuwei Ge Yiming Zeng Zanjing Zhai Congqin Ning Huiwu Li |
| author_sort | Jingwei Zhang |
| collection | DOAJ |
| description | Background: Calcium phosphate silicate (Ca5(PO4)2SiO4 or CPS) is a promising bioceramic for bone grafting. Iron (Fe) is a trace element in the human body that has been reported to enhance the mechanical strength of CPS ceramics. However, the exact biofunctions of Fe, combined with another human trace element, viz. silicon (Si), in CPS and the optimal dose for Fe addition must be further investigated. Methods: In vitro: the morphology, structure and cell adhesion were observed by SEM; the ability to promote osteogenic differentiation and mineralization was explored by ALP and alizarin red staining; the expression of osteogenic-specific genes and proteins was detected by PCR, WB and immunofluorescence. In vivo: Further exploration of bone regeneration capacity by establishing a skull defect model. Results: In vitro, we observed increased content of adhesion-related proteins and osteogenic-related genes expression of Fe-CPS compared with CPS, as demonstrated by immunofluorescence and polymerase chain reaction experiments, respectively. In vivo micro-computed tomography images, histomorphology, and undecalcified bone slicing also showed improved osteogenic ability of Fe-CPS bioceramics. Conclusion: With the addition of Fe2O3, the new bone formation rate of the Fe-CPS scaffold after 12 weeks increased from 9.42% to 43.76%. Moreover, both in vitro and in vivo experimental outcomes indicated that Fe addition improved the CPS bioceramics in terms of their osteogenic ability by promoting the expression of osteogenic-related genes. Fe-CPS bioceramics can be employed as a novel material for bone tissue engineering on account of their outstanding new bone formation ability. The translational potential of this article: This study suggests that Fe-CPS bioceramics can be employed as a novel material for bone tissue engineering on account of their outstanding new bone formation ability,which provides promising therapeutic implants and strategies for the treatment of large segmental bone defects. |
| first_indexed | 2024-12-22T21:12:43Z |
| format | Article |
| id | doaj.art-dc2e025a87bb4f86ac7a2c61bec0062f |
| institution | Directory Open Access Journal |
| issn | 2214-031X |
| language | English |
| last_indexed | 2024-12-22T21:12:43Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Orthopaedic Translation |
| spelling | doaj.art-dc2e025a87bb4f86ac7a2c61bec0062f2022-12-21T18:12:29ZengElsevierJournal of Orthopaedic Translation2214-031X2022-01-0132103111Favorable osteogenic activity of iron doped in silicocarnotite bioceramic: In vitro and in vivo StudiesJingwei Zhang0Fanyan Deng1Xiaoliang Liu2Yuwei Ge3Yiming Zeng4Zanjing Zhai5Congqin Ning6Huiwu Li7Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639# Zhizaoju Road, Shanghai, 200011, PR ChinaState Key Laboratory of High Performance Ceramics and Superfine Microstructure, Institute of Ceramics, Chinese Academy of Sciences, 1295# Dingxi Road, Shanghai, 200050, ChinaShanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639# Zhizaoju Road, Shanghai, 200011, PR ChinaDepartment of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600# Yishan Road, Shanghai, 200233, ChinaShanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639# Zhizaoju Road, Shanghai, 200011, PR ChinaShanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639# Zhizaoju Road, Shanghai, 200011, PR ChinaThe Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, 100# Guilin Road, Shanghai, 200234, China; Corresponding author.Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639# Zhizaoju Road, Shanghai, 200011, PR China; Corresponding author.Background: Calcium phosphate silicate (Ca5(PO4)2SiO4 or CPS) is a promising bioceramic for bone grafting. Iron (Fe) is a trace element in the human body that has been reported to enhance the mechanical strength of CPS ceramics. However, the exact biofunctions of Fe, combined with another human trace element, viz. silicon (Si), in CPS and the optimal dose for Fe addition must be further investigated. Methods: In vitro: the morphology, structure and cell adhesion were observed by SEM; the ability to promote osteogenic differentiation and mineralization was explored by ALP and alizarin red staining; the expression of osteogenic-specific genes and proteins was detected by PCR, WB and immunofluorescence. In vivo: Further exploration of bone regeneration capacity by establishing a skull defect model. Results: In vitro, we observed increased content of adhesion-related proteins and osteogenic-related genes expression of Fe-CPS compared with CPS, as demonstrated by immunofluorescence and polymerase chain reaction experiments, respectively. In vivo micro-computed tomography images, histomorphology, and undecalcified bone slicing also showed improved osteogenic ability of Fe-CPS bioceramics. Conclusion: With the addition of Fe2O3, the new bone formation rate of the Fe-CPS scaffold after 12 weeks increased from 9.42% to 43.76%. Moreover, both in vitro and in vivo experimental outcomes indicated that Fe addition improved the CPS bioceramics in terms of their osteogenic ability by promoting the expression of osteogenic-related genes. Fe-CPS bioceramics can be employed as a novel material for bone tissue engineering on account of their outstanding new bone formation ability. The translational potential of this article: This study suggests that Fe-CPS bioceramics can be employed as a novel material for bone tissue engineering on account of their outstanding new bone formation ability,which provides promising therapeutic implants and strategies for the treatment of large segmental bone defects.http://www.sciencedirect.com/science/article/pii/S2214031X21001054BioceramicBone formationIronOsteogenic activitySilicocarnotite |
| spellingShingle | Jingwei Zhang Fanyan Deng Xiaoliang Liu Yuwei Ge Yiming Zeng Zanjing Zhai Congqin Ning Huiwu Li Favorable osteogenic activity of iron doped in silicocarnotite bioceramic: In vitro and in vivo Studies Journal of Orthopaedic Translation Bioceramic Bone formation Iron Osteogenic activity Silicocarnotite |
| title | Favorable osteogenic activity of iron doped in silicocarnotite bioceramic: In vitro and in vivo Studies |
| title_full | Favorable osteogenic activity of iron doped in silicocarnotite bioceramic: In vitro and in vivo Studies |
| title_fullStr | Favorable osteogenic activity of iron doped in silicocarnotite bioceramic: In vitro and in vivo Studies |
| title_full_unstemmed | Favorable osteogenic activity of iron doped in silicocarnotite bioceramic: In vitro and in vivo Studies |
| title_short | Favorable osteogenic activity of iron doped in silicocarnotite bioceramic: In vitro and in vivo Studies |
| title_sort | favorable osteogenic activity of iron doped in silicocarnotite bioceramic in vitro and in vivo studies |
| topic | Bioceramic Bone formation Iron Osteogenic activity Silicocarnotite |
| url | http://www.sciencedirect.com/science/article/pii/S2214031X21001054 |
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