The Effect of Diosmin, Escin, and Bromelain on Human Endothelial Cells Derived from the Umbilical Vein and the Varicose Vein—A Preliminary Study
In this study, we investigated the properties of human varicose vein (VV) endothelial cells (HVVEC) in comparison to the human umbilical vein endothelial cells (HUVEC). The cells were treated with three bioactive compounds with proven beneficial effects in the therapy of patients with VV, diosmin, e...
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MDPI AG
2023-06-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/6/1702 |
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| author | Lukasz Gwozdzinski Joanna Bernasinska-Slomczewska Pawel Hikisz Anna Wiktorowska-Owczarek Edward Kowalczyk Anna Pieniazek |
| author_facet | Lukasz Gwozdzinski Joanna Bernasinska-Slomczewska Pawel Hikisz Anna Wiktorowska-Owczarek Edward Kowalczyk Anna Pieniazek |
| author_sort | Lukasz Gwozdzinski |
| collection | DOAJ |
| description | In this study, we investigated the properties of human varicose vein (VV) endothelial cells (HVVEC) in comparison to the human umbilical vein endothelial cells (HUVEC). The cells were treated with three bioactive compounds with proven beneficial effects in the therapy of patients with VV, diosmin, escin, and bromelain. Two concentrations of tested drugs were used (1, 10 mg/mL), which did not affect the viability of either cell type. Escin led to a slight generation of reactive oxygen species in HUVEC cells. We observed a slight release of superoxide in HVVEC cells upon treatment with diosmin and escin. Diosmin and bromelain showed a tendency to release nitric oxide in HUVEC. Using membrane fluorescent probes, we demonstrated a reduced fluidity of HVVEC, which may lead to their increased adhesion, and, consequently, a much more frequent occurrence of venous thrombosis. For the first time, we show the mechanism of action of drugs used in VV therapy on endothelial cells derived from a VV. Studies with HVVEC have shown that tested drugs may lead to a reduction in the adhesive properties of these cells, and thus to a lower risk of thrombosis. |
| first_indexed | 2024-03-11T02:44:10Z |
| format | Article |
| id | doaj.art-dc2f84c36b404066a804ede446389f2f |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T02:44:10Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-dc2f84c36b404066a804ede446389f2f2023-11-18T09:27:00ZengMDPI AGBiomedicines2227-90592023-06-01116170210.3390/biomedicines11061702The Effect of Diosmin, Escin, and Bromelain on Human Endothelial Cells Derived from the Umbilical Vein and the Varicose Vein—A Preliminary StudyLukasz Gwozdzinski0Joanna Bernasinska-Slomczewska1Pawel Hikisz2Anna Wiktorowska-Owczarek3Edward Kowalczyk4Anna Pieniazek5Department of Pharmacology and Toxicology, Medical University of Lodz, 90-752 Lodz, PolandDepartment of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Pharmacology and Toxicology, Medical University of Lodz, 90-752 Lodz, PolandDepartment of Pharmacology and Toxicology, Medical University of Lodz, 90-752 Lodz, PolandDepartment of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandIn this study, we investigated the properties of human varicose vein (VV) endothelial cells (HVVEC) in comparison to the human umbilical vein endothelial cells (HUVEC). The cells were treated with three bioactive compounds with proven beneficial effects in the therapy of patients with VV, diosmin, escin, and bromelain. Two concentrations of tested drugs were used (1, 10 mg/mL), which did not affect the viability of either cell type. Escin led to a slight generation of reactive oxygen species in HUVEC cells. We observed a slight release of superoxide in HVVEC cells upon treatment with diosmin and escin. Diosmin and bromelain showed a tendency to release nitric oxide in HUVEC. Using membrane fluorescent probes, we demonstrated a reduced fluidity of HVVEC, which may lead to their increased adhesion, and, consequently, a much more frequent occurrence of venous thrombosis. For the first time, we show the mechanism of action of drugs used in VV therapy on endothelial cells derived from a VV. Studies with HVVEC have shown that tested drugs may lead to a reduction in the adhesive properties of these cells, and thus to a lower risk of thrombosis.https://www.mdpi.com/2227-9059/11/6/1702varicose veinhuman endothelial cellsdiosminescinbromelain |
| spellingShingle | Lukasz Gwozdzinski Joanna Bernasinska-Slomczewska Pawel Hikisz Anna Wiktorowska-Owczarek Edward Kowalczyk Anna Pieniazek The Effect of Diosmin, Escin, and Bromelain on Human Endothelial Cells Derived from the Umbilical Vein and the Varicose Vein—A Preliminary Study Biomedicines varicose vein human endothelial cells diosmin escin bromelain |
| title | The Effect of Diosmin, Escin, and Bromelain on Human Endothelial Cells Derived from the Umbilical Vein and the Varicose Vein—A Preliminary Study |
| title_full | The Effect of Diosmin, Escin, and Bromelain on Human Endothelial Cells Derived from the Umbilical Vein and the Varicose Vein—A Preliminary Study |
| title_fullStr | The Effect of Diosmin, Escin, and Bromelain on Human Endothelial Cells Derived from the Umbilical Vein and the Varicose Vein—A Preliminary Study |
| title_full_unstemmed | The Effect of Diosmin, Escin, and Bromelain on Human Endothelial Cells Derived from the Umbilical Vein and the Varicose Vein—A Preliminary Study |
| title_short | The Effect of Diosmin, Escin, and Bromelain on Human Endothelial Cells Derived from the Umbilical Vein and the Varicose Vein—A Preliminary Study |
| title_sort | effect of diosmin escin and bromelain on human endothelial cells derived from the umbilical vein and the varicose vein a preliminary study |
| topic | varicose vein human endothelial cells diosmin escin bromelain |
| url | https://www.mdpi.com/2227-9059/11/6/1702 |
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