Prostate cancer diagnosis: an immunological aspects
Introduction. Contemporary diagnosis of prostate cancer is crucial to the patient’s further fate. Difficulties in the histological verification of the final diagnosis and false-negative results of biopsy research are often associated with the similarity of the prostate adenocarcinoma`s micromorpholo...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
State Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian Federation
2019-02-01
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| Series: | Vestnik Urologii |
| Subjects: | |
| Online Access: | https://www.urovest.ru/jour/article/view/235 |
| _version_ | 1797272180906524672 |
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| author | S. V. Popov R. G. Guseinov I. N. Orlov V. V. Protoshchak O. N. Skryabin P. A. Babkin F. A. Bagrov A. S. Katunin A. G. Martov V. L. Schukin |
| author_facet | S. V. Popov R. G. Guseinov I. N. Orlov V. V. Protoshchak O. N. Skryabin P. A. Babkin F. A. Bagrov A. S. Katunin A. G. Martov V. L. Schukin |
| author_sort | S. V. Popov |
| collection | DOAJ |
| description | Introduction. Contemporary diagnosis of prostate cancer is crucial to the patient’s further fate. Difficulties in the histological verification of the final diagnosis and false-negative results of biopsy research are often associated with the similarity of the prostate adenocarcinoma`s micromorphological picture and its benign lesions.Purpose of the study. Comprehending the possibilities of immunohistochemical identification of tumor cells and the basal epithelial layer of prostate glandular structures in prostate cancer suspected cases.Materials and methods. A biopsy material was taken from 134 patients. Prostate adenocarcinoma was verified by routine histological examination in 72 samples, in 62 samples there were no signs of malignant transformation. Subsequently, immunohistochemical analysis of biopsy specimens was performed using antibodies to α-methylacyl-CoA racemase, nuclear p63 protein and high-molecular cytokeratin.Results. The AMACR-positive reaction of malignant cells and a negative reaction of the basal epithelium to nuclear p63 protein antibodies and high-molecular cytokeratin were detected during prostate adenocarcinoma.Conclusion. If prostate cancer is suspected, immunohistochemical assays with monoclonal antibodies to AMACR, p63 and high-molecular cytokeratin greatly facilitate the detection of adenocarcinoma. |
| first_indexed | 2024-03-07T14:24:42Z |
| format | Article |
| id | doaj.art-dc34432ea94e4de69534ebaf82f4fa20 |
| institution | Directory Open Access Journal |
| issn | 2308-6424 |
| language | Russian |
| last_indexed | 2024-03-07T14:24:42Z |
| publishDate | 2019-02-01 |
| publisher | State Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian Federation |
| record_format | Article |
| series | Vestnik Urologii |
| spelling | doaj.art-dc34432ea94e4de69534ebaf82f4fa202024-03-06T08:56:03ZrusState Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian FederationVestnik Urologii2308-64242019-02-0164424810.21886/2308-6424-2018-6-4-42-48181Prostate cancer diagnosis: an immunological aspectsS. V. Popov0R. G. Guseinov1I. N. Orlov2V. V. Protoshchak3O. N. Skryabin4P. A. Babkin5F. A. Bagrov6A. S. Katunin7A. G. Martov8V. L. Schukin9Clinical Hospital of St. Luke.Clinical Hospital of St. Luke.Clinical Hospital of St. Luke.Military medical academy of S.M. Kirov.Clinical Hospital of St. Luke.Military medical academy of S.M. Kirov.Clinical Hospital of St. Luke.Clinical Hospital of St. Luke.State Research Center – Burnasyan Federal Medical Biophysical Center.The City Hospital No. 20.Introduction. Contemporary diagnosis of prostate cancer is crucial to the patient’s further fate. Difficulties in the histological verification of the final diagnosis and false-negative results of biopsy research are often associated with the similarity of the prostate adenocarcinoma`s micromorphological picture and its benign lesions.Purpose of the study. Comprehending the possibilities of immunohistochemical identification of tumor cells and the basal epithelial layer of prostate glandular structures in prostate cancer suspected cases.Materials and methods. A biopsy material was taken from 134 patients. Prostate adenocarcinoma was verified by routine histological examination in 72 samples, in 62 samples there were no signs of malignant transformation. Subsequently, immunohistochemical analysis of biopsy specimens was performed using antibodies to α-methylacyl-CoA racemase, nuclear p63 protein and high-molecular cytokeratin.Results. The AMACR-positive reaction of malignant cells and a negative reaction of the basal epithelium to nuclear p63 protein antibodies and high-molecular cytokeratin were detected during prostate adenocarcinoma.Conclusion. If prostate cancer is suspected, immunohistochemical assays with monoclonal antibodies to AMACR, p63 and high-molecular cytokeratin greatly facilitate the detection of adenocarcinoma.https://www.urovest.ru/jour/article/view/235prostate adenocarcinomabiopsyfalse-negative resultsamacrp6334βe12 |
| spellingShingle | S. V. Popov R. G. Guseinov I. N. Orlov V. V. Protoshchak O. N. Skryabin P. A. Babkin F. A. Bagrov A. S. Katunin A. G. Martov V. L. Schukin Prostate cancer diagnosis: an immunological aspects Vestnik Urologii prostate adenocarcinoma biopsy false-negative results amacr p63 34βe12 |
| title | Prostate cancer diagnosis: an immunological aspects |
| title_full | Prostate cancer diagnosis: an immunological aspects |
| title_fullStr | Prostate cancer diagnosis: an immunological aspects |
| title_full_unstemmed | Prostate cancer diagnosis: an immunological aspects |
| title_short | Prostate cancer diagnosis: an immunological aspects |
| title_sort | prostate cancer diagnosis an immunological aspects |
| topic | prostate adenocarcinoma biopsy false-negative results amacr p63 34βe12 |
| url | https://www.urovest.ru/jour/article/view/235 |
| work_keys_str_mv | AT svpopov prostatecancerdiagnosisanimmunologicalaspects AT rgguseinov prostatecancerdiagnosisanimmunologicalaspects AT inorlov prostatecancerdiagnosisanimmunologicalaspects AT vvprotoshchak prostatecancerdiagnosisanimmunologicalaspects AT onskryabin prostatecancerdiagnosisanimmunologicalaspects AT pababkin prostatecancerdiagnosisanimmunologicalaspects AT fabagrov prostatecancerdiagnosisanimmunologicalaspects AT askatunin prostatecancerdiagnosisanimmunologicalaspects AT agmartov prostatecancerdiagnosisanimmunologicalaspects AT vlschukin prostatecancerdiagnosisanimmunologicalaspects |