Association between MTHFR C677T Gene Polymorphisms and the Efficacy of Vitamin Therapy in lowering Homocysteine Levels among Stroke Patients with Hyperhomocysteinemia
Background: The impact of the methylenetetrahydrofolate reductase (MTHFR) C677T mutation on the relationship between plasma homocysteine (Hcy) levels and stroke has been extensively studied and documented in previous study. However, it remains unclear whether the MTHFR C677T mutation can affect the...
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| Format: | Article |
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IMR Press
2024-01-01
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| Series: | Journal of Integrative Neuroscience |
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| Online Access: | https://www.imrpress.com/journal/JIN/23/1/10.31083/j.jin2301003 |
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| author | Zhi-Can Li Min Huang Qing-Yang Yao Cai-Hong Lin Bing-Cong Hong Jie-Hua Wang Zedan Zhang |
| author_facet | Zhi-Can Li Min Huang Qing-Yang Yao Cai-Hong Lin Bing-Cong Hong Jie-Hua Wang Zedan Zhang |
| author_sort | Zhi-Can Li |
| collection | DOAJ |
| description | Background: The impact of the methylenetetrahydrofolate reductase (MTHFR) C677T mutation on the relationship between plasma homocysteine (Hcy) levels and stroke has been extensively studied and documented in previous study. However, it remains unclear whether the MTHFR C677T mutation can affect the response to Hcy lowering treatment in stroke patients with hyperhomocysteinemia (HHcy). Understanding the impact of genetic factors on treatment response can help optimize personalized treatment strategies for stroke patients with HHcy. We aimed to investigate the potential association between the MTHFR C677T gene polymorphisms and the effectiveness of Hcy lowering treatment using vitamin therapy in stroke patients with HHcy. Methods: The MTHFR C677T genotype polymorphisms were identified using polymerase chain reaction-restriction fragment length polymorphism, and the distribution of three genotypes in the MTHFR C677T gene locus was compared. The treatment effects of Hcy lowering agents were compared among patients with different genotypes. Results: Among the 320 stroke patients enrolled in the study, 258 (80.6%) were diagnosed with HHcy. Of these, 162 patients (Effective Group) responded well to the clinical Hcy lowering treatment, while 96 patients (Invalid Group) failed to achieve sufficient response even after taking combination supplements of folic acid, Vitamin B6, and methylcobalamin for one month. Significant differences were observed in terms of age (p < 0.001), hypertension (p = 0.034), dyslipidemia (p = 0.022), hyperuricemia (p = 0.013) and genotype distribution of MTHFR C677T gene polymorphism (p < 0.001) between the Invalid group and the Effective group. The multivariate regression analysis revealed that the T allele (odd rations [OR], 1.327; 95% confidence interval [CI], 1.114–1.580; p = 0.0015) was independently associated with an insufficient Hcy lowering treatment effect. Additionally, the TT genotype was independently associated with insufficient response in both the codominant model (OR, 1.645; 95% CI, 1.093–2.476; p = 0.017) and the recessive model (TT versus CC + CT; OR, 1.529; 95% CI, 1.145–2.042; p = 0.004). However, no relationship was observed between CT + TT genotypes and poor treatment effect in the dominate model. Conclusions: Our findings suggested that the TT genotype and T allele of MTHFR C677T polymorphism were independently associated with an insufficient Hcy lowering treatment effect in stroke patients with HHcy. |
| first_indexed | 2024-03-08T09:37:16Z |
| format | Article |
| id | doaj.art-dc3587d132d74b5eaca99c9c8f804dee |
| institution | Directory Open Access Journal |
| issn | 0219-6352 |
| language | English |
| last_indexed | 2024-03-08T09:37:16Z |
| publishDate | 2024-01-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Journal of Integrative Neuroscience |
| spelling | doaj.art-dc3587d132d74b5eaca99c9c8f804dee2024-01-30T07:44:42ZengIMR PressJournal of Integrative Neuroscience0219-63522024-01-01231310.31083/j.jin2301003S0219-6352(23)00562-4Association between MTHFR C677T Gene Polymorphisms and the Efficacy of Vitamin Therapy in lowering Homocysteine Levels among Stroke Patients with HyperhomocysteinemiaZhi-Can Li0Min Huang1Qing-Yang Yao2Cai-Hong Lin3Bing-Cong Hong4Jie-Hua Wang5Zedan Zhang6Department of Neurology, Quanzhou First Hospital Affiliated to Fujian Medical University, 362000 Quanzhou, Fujian, ChinaDepartment of Geriatrics, The 900th Hospital of Joint Logistics Support Force of Chinese PLA, 350025 Fuzhou, Fujian, ChinaDepartment of Neurology, Quanzhou First Hospital Affiliated to Fujian Medical University, 362000 Quanzhou, Fujian, ChinaDepartment of Neurology, Quanzhou First Hospital Affiliated to Fujian Medical University, 362000 Quanzhou, Fujian, ChinaDepartment of Neurology, Quanzhou First Hospital Affiliated to Fujian Medical University, 362000 Quanzhou, Fujian, ChinaDepartment of Neurology, Quanzhou First Hospital Affiliated to Fujian Medical University, 362000 Quanzhou, Fujian, ChinaDepartment of Health Medicine, The 900th Hospital of Joint Logistics Support Force of Chinese PLA, 350025 Fuzhou, Fujian, ChinaBackground: The impact of the methylenetetrahydrofolate reductase (MTHFR) C677T mutation on the relationship between plasma homocysteine (Hcy) levels and stroke has been extensively studied and documented in previous study. However, it remains unclear whether the MTHFR C677T mutation can affect the response to Hcy lowering treatment in stroke patients with hyperhomocysteinemia (HHcy). Understanding the impact of genetic factors on treatment response can help optimize personalized treatment strategies for stroke patients with HHcy. We aimed to investigate the potential association between the MTHFR C677T gene polymorphisms and the effectiveness of Hcy lowering treatment using vitamin therapy in stroke patients with HHcy. Methods: The MTHFR C677T genotype polymorphisms were identified using polymerase chain reaction-restriction fragment length polymorphism, and the distribution of three genotypes in the MTHFR C677T gene locus was compared. The treatment effects of Hcy lowering agents were compared among patients with different genotypes. Results: Among the 320 stroke patients enrolled in the study, 258 (80.6%) were diagnosed with HHcy. Of these, 162 patients (Effective Group) responded well to the clinical Hcy lowering treatment, while 96 patients (Invalid Group) failed to achieve sufficient response even after taking combination supplements of folic acid, Vitamin B6, and methylcobalamin for one month. Significant differences were observed in terms of age (p < 0.001), hypertension (p = 0.034), dyslipidemia (p = 0.022), hyperuricemia (p = 0.013) and genotype distribution of MTHFR C677T gene polymorphism (p < 0.001) between the Invalid group and the Effective group. The multivariate regression analysis revealed that the T allele (odd rations [OR], 1.327; 95% confidence interval [CI], 1.114–1.580; p = 0.0015) was independently associated with an insufficient Hcy lowering treatment effect. Additionally, the TT genotype was independently associated with insufficient response in both the codominant model (OR, 1.645; 95% CI, 1.093–2.476; p = 0.017) and the recessive model (TT versus CC + CT; OR, 1.529; 95% CI, 1.145–2.042; p = 0.004). However, no relationship was observed between CT + TT genotypes and poor treatment effect in the dominate model. Conclusions: Our findings suggested that the TT genotype and T allele of MTHFR C677T polymorphism were independently associated with an insufficient Hcy lowering treatment effect in stroke patients with HHcy.https://www.imrpress.com/journal/JIN/23/1/10.31083/j.jin2301003strokehomocysteinesingle nucleotide polymorphismmethylenetetrahydrofolate reductase |
| spellingShingle | Zhi-Can Li Min Huang Qing-Yang Yao Cai-Hong Lin Bing-Cong Hong Jie-Hua Wang Zedan Zhang Association between MTHFR C677T Gene Polymorphisms and the Efficacy of Vitamin Therapy in lowering Homocysteine Levels among Stroke Patients with Hyperhomocysteinemia Journal of Integrative Neuroscience stroke homocysteine single nucleotide polymorphism methylenetetrahydrofolate reductase |
| title | Association between MTHFR C677T Gene Polymorphisms and the Efficacy of Vitamin Therapy in lowering Homocysteine Levels among Stroke Patients with Hyperhomocysteinemia |
| title_full | Association between MTHFR C677T Gene Polymorphisms and the Efficacy of Vitamin Therapy in lowering Homocysteine Levels among Stroke Patients with Hyperhomocysteinemia |
| title_fullStr | Association between MTHFR C677T Gene Polymorphisms and the Efficacy of Vitamin Therapy in lowering Homocysteine Levels among Stroke Patients with Hyperhomocysteinemia |
| title_full_unstemmed | Association between MTHFR C677T Gene Polymorphisms and the Efficacy of Vitamin Therapy in lowering Homocysteine Levels among Stroke Patients with Hyperhomocysteinemia |
| title_short | Association between MTHFR C677T Gene Polymorphisms and the Efficacy of Vitamin Therapy in lowering Homocysteine Levels among Stroke Patients with Hyperhomocysteinemia |
| title_sort | association between mthfr c677t gene polymorphisms and the efficacy of vitamin therapy in lowering homocysteine levels among stroke patients with hyperhomocysteinemia |
| topic | stroke homocysteine single nucleotide polymorphism methylenetetrahydrofolate reductase |
| url | https://www.imrpress.com/journal/JIN/23/1/10.31083/j.jin2301003 |
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