Detection of the blaVIM-2 Gene in Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates in Sudan
Background: Acinetobacter baumannii is a pleomorphic aerobic Gram-negative bacillus that is notorious for having multidrug resistance traits. Carbapenem-resistant Acinetobacter baumannii (CRAB) is a global concern due to its ability to retain and disseminate resistance genes, which poses a threat to...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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International Medical Research and Development Corporation
2022-12-01
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| Series: | International Journal of Biomedicine |
| Subjects: | |
| Online Access: | http://www.ijbm.org/articles/i48/ijbm_12(4)_oa21.pdf |
| _version_ | 1811178025564241920 |
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| author | Salma Mohamed Zainab Ahmed Tajalseer Mubarak Sara Mohamed Hassan Higazi Sara Ali |
| author_facet | Salma Mohamed Zainab Ahmed Tajalseer Mubarak Sara Mohamed Hassan Higazi Sara Ali |
| author_sort | Salma Mohamed |
| collection | DOAJ |
| description | Background: Acinetobacter baumannii is a pleomorphic aerobic Gram-negative bacillus that is notorious for having multidrug resistance traits. Carbapenem-resistant Acinetobacter baumannii (CRAB) is a global concern due to its ability to retain and disseminate resistance genes, which poses a threat to the spread of resistance among bacterial communities in hospital settings. The aim of our study was to evaluate the existence of the blaVIM-2 gene in carbapenem-resistant Acinetobacter baumannii clinical isolates from Sudanese hospitals.
Methods and Results: Forty clinical isolates of Acinetobacter baumannii were collected from June 2021 to April 2022. All isolates of Acinetobacter baumannii were identified via BioMérieux's Vitek-2 automated system (Marcy l'Étoile, France) and evaluated for phenotypic resistance to carbapenem, using imipenem and meropenem. The enzymatic mode of resistance was assessed by the Modified Hodge test (MHT). A real-time PCR was used to detect the presence of the blaVIM-2 gene.
Of 40 isolates, 32(80%) were resistant to imipenem, 4(10%) were moderately resistant, and 4(10%) were susceptible to imipenem; 24(60%) were resistant to meropenem, 10(25%) were moderately resistant, and 6(15%) were susceptible to meropenem. MHT was 70% positive with imipenem use and 55% positive with meropenem use. Real-time PCR revealed that only 30% of the samples were positive for the blaVIM-2 gene. All blaVIM-2-positive isolates were resistant to both imipenem and meropenem.
Conclusion: Resistance to carbapenem poses a serious threat, denying patients treatment options. It is essential to make continuous surveillance of these strains to prevent the development of resistant strains. |
| first_indexed | 2024-04-11T06:12:29Z |
| format | Article |
| id | doaj.art-dc3a53470c13434c9341d6238b3e18ef |
| institution | Directory Open Access Journal |
| issn | 2158-0510 2158-0529 |
| language | English |
| last_indexed | 2024-04-11T06:12:29Z |
| publishDate | 2022-12-01 |
| publisher | International Medical Research and Development Corporation |
| record_format | Article |
| series | International Journal of Biomedicine |
| spelling | doaj.art-dc3a53470c13434c9341d6238b3e18ef2022-12-22T04:41:14ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292022-12-0112463663910.21103/Article12(4)_OA21Detection of the blaVIM-2 Gene in Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates in SudanSalma Mohamed0Zainab Ahmed1Tajalseer Mubarak2Sara Mohamed3Hassan Higazi4Sara Ali5Department of Medical Laboratory Science, Faculty of Health Sciences, Gulf Medical University, Ajman, United Arab EmiratesDepartment of Microbiology, Faculty of Medical Laboratory Science, University of Science and Technology, Khartoum, SudanDepartment of Microbiology, Faculty of Medical Laboratory Science, University of Science and Technology, Khartoum, SudanDeparment of Internal Medicine, Faculty of Medicine, Al Neelain University, Khartoum, SudanDepartment of Medical Laboratory Science, Faculty of Health Sciences, Gulf Medical University, Ajman, United Arab EmiratesDepartment of Medical Laboratory Science, Faculty of Health Sciences, Gulf Medical University, Ajman, United Arab EmiratesBackground: Acinetobacter baumannii is a pleomorphic aerobic Gram-negative bacillus that is notorious for having multidrug resistance traits. Carbapenem-resistant Acinetobacter baumannii (CRAB) is a global concern due to its ability to retain and disseminate resistance genes, which poses a threat to the spread of resistance among bacterial communities in hospital settings. The aim of our study was to evaluate the existence of the blaVIM-2 gene in carbapenem-resistant Acinetobacter baumannii clinical isolates from Sudanese hospitals. Methods and Results: Forty clinical isolates of Acinetobacter baumannii were collected from June 2021 to April 2022. All isolates of Acinetobacter baumannii were identified via BioMérieux's Vitek-2 automated system (Marcy l'Étoile, France) and evaluated for phenotypic resistance to carbapenem, using imipenem and meropenem. The enzymatic mode of resistance was assessed by the Modified Hodge test (MHT). A real-time PCR was used to detect the presence of the blaVIM-2 gene. Of 40 isolates, 32(80%) were resistant to imipenem, 4(10%) were moderately resistant, and 4(10%) were susceptible to imipenem; 24(60%) were resistant to meropenem, 10(25%) were moderately resistant, and 6(15%) were susceptible to meropenem. MHT was 70% positive with imipenem use and 55% positive with meropenem use. Real-time PCR revealed that only 30% of the samples were positive for the blaVIM-2 gene. All blaVIM-2-positive isolates were resistant to both imipenem and meropenem. Conclusion: Resistance to carbapenem poses a serious threat, denying patients treatment options. It is essential to make continuous surveillance of these strains to prevent the development of resistant strains.http://www.ijbm.org/articles/i48/ijbm_12(4)_oa21.pdfcarbapenem-resistant acinetobacter baumannblavim-2 genecarbapenemases |
| spellingShingle | Salma Mohamed Zainab Ahmed Tajalseer Mubarak Sara Mohamed Hassan Higazi Sara Ali Detection of the blaVIM-2 Gene in Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates in Sudan International Journal of Biomedicine carbapenem-resistant acinetobacter baumann blavim-2 gene carbapenemases |
| title | Detection of the blaVIM-2 Gene in Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates in Sudan |
| title_full | Detection of the blaVIM-2 Gene in Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates in Sudan |
| title_fullStr | Detection of the blaVIM-2 Gene in Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates in Sudan |
| title_full_unstemmed | Detection of the blaVIM-2 Gene in Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates in Sudan |
| title_short | Detection of the blaVIM-2 Gene in Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates in Sudan |
| title_sort | detection of the blavim 2 gene in carbapenem resistant acinetobacter baumannii clinical isolates in sudan |
| topic | carbapenem-resistant acinetobacter baumann blavim-2 gene carbapenemases |
| url | http://www.ijbm.org/articles/i48/ijbm_12(4)_oa21.pdf |
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