Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.
<h4>Background</h4>The present study aims to identify antigens in protein extracts of promastigote and amastigote-like Leishmania (Leishmania) chagasi syn. L. (L.) infantum recognized by antibodies present in the sera of dogs with asymptomatic and symptomatic visceral leishmaniasis (VL)....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22272364/pdf/?tool=EBI |
| _version_ | 1818402877784195072 |
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| author | Vinicio T S Coelho Jamil S Oliveira Diogo G Valadares Miguel A Chávez-Fumagalli Mariana C Duarte Paula S Lage Manuel Soto Marcelo M Santoro Carlos A P Tavares Ana Paula Fernandes Eduardo A F Coelho |
| author_facet | Vinicio T S Coelho Jamil S Oliveira Diogo G Valadares Miguel A Chávez-Fumagalli Mariana C Duarte Paula S Lage Manuel Soto Marcelo M Santoro Carlos A P Tavares Ana Paula Fernandes Eduardo A F Coelho |
| author_sort | Vinicio T S Coelho |
| collection | DOAJ |
| description | <h4>Background</h4>The present study aims to identify antigens in protein extracts of promastigote and amastigote-like Leishmania (Leishmania) chagasi syn. L. (L.) infantum recognized by antibodies present in the sera of dogs with asymptomatic and symptomatic visceral leishmaniasis (VL).<h4>Methodology/principal findings</h4>Proteins recognized by sera samples were separated by two-dimensional electrophoresis (2DE) and identified by mass spectrometry. A total of 550 spots were observed in the 2DE gels, and approximately 104 proteins were identified. Several stage-specific proteins could be identified by either or both classes of sera, including, as expected, previously known proteins identified as diagnosis, virulence factors, drug targets, or vaccine candidates. Three, seven, and five hypothetical proteins could be identified in promastigote antigenic extracts; while two, eleven, and three hypothetical proteins could be identified in amastigote-like antigenic extracts by asymptomatic and symptomatic sera, as well as a combination of both, respectively.<h4>Conclusions/significance</h4>The present study represents a significant contribution not only in identifying stage-specific L. infantum molecules, but also in revealing the expression of a large number of hypothetical proteins. Moreover, when combined, the identified proteins constitute a significant source of information for the improvement of diagnostic tools and/or vaccine development to VL. |
| first_indexed | 2024-12-14T08:15:21Z |
| format | Article |
| id | doaj.art-dc416b86cf8d46cc80acdda37c4f26e6 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-14T08:15:21Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-dc416b86cf8d46cc80acdda37c4f26e62022-12-21T23:09:57ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352012-01-0161e143010.1371/journal.pntd.0001430Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.Vinicio T S CoelhoJamil S OliveiraDiogo G ValadaresMiguel A Chávez-FumagalliMariana C DuartePaula S LageManuel SotoMarcelo M SantoroCarlos A P TavaresAna Paula FernandesEduardo A F Coelho<h4>Background</h4>The present study aims to identify antigens in protein extracts of promastigote and amastigote-like Leishmania (Leishmania) chagasi syn. L. (L.) infantum recognized by antibodies present in the sera of dogs with asymptomatic and symptomatic visceral leishmaniasis (VL).<h4>Methodology/principal findings</h4>Proteins recognized by sera samples were separated by two-dimensional electrophoresis (2DE) and identified by mass spectrometry. A total of 550 spots were observed in the 2DE gels, and approximately 104 proteins were identified. Several stage-specific proteins could be identified by either or both classes of sera, including, as expected, previously known proteins identified as diagnosis, virulence factors, drug targets, or vaccine candidates. Three, seven, and five hypothetical proteins could be identified in promastigote antigenic extracts; while two, eleven, and three hypothetical proteins could be identified in amastigote-like antigenic extracts by asymptomatic and symptomatic sera, as well as a combination of both, respectively.<h4>Conclusions/significance</h4>The present study represents a significant contribution not only in identifying stage-specific L. infantum molecules, but also in revealing the expression of a large number of hypothetical proteins. Moreover, when combined, the identified proteins constitute a significant source of information for the improvement of diagnostic tools and/or vaccine development to VL.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22272364/pdf/?tool=EBI |
| spellingShingle | Vinicio T S Coelho Jamil S Oliveira Diogo G Valadares Miguel A Chávez-Fumagalli Mariana C Duarte Paula S Lage Manuel Soto Marcelo M Santoro Carlos A P Tavares Ana Paula Fernandes Eduardo A F Coelho Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach. PLoS Neglected Tropical Diseases |
| title | Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach. |
| title_full | Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach. |
| title_fullStr | Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach. |
| title_full_unstemmed | Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach. |
| title_short | Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach. |
| title_sort | identification of proteins in promastigote and amastigote like leishmania using an immunoproteomic approach |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22272364/pdf/?tool=EBI |
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