The Effect of High-Dose-Rate Pulsed Radiation on the Survival of Clinically Relevant Radioresistant Cells
We demonstrated that low dose pulsed radiation (0.25 Gy) at a high-dose-rate, even for very short intervals (10 s), decreases cell survival to a greater extent than single exposure to a similar total dose and dose rate. The objective of this study was to clarify whether high-dose-rate pulsed radiati...
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MDPI AG
2021-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/12/1295 |
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| author | Shingo Terashima Hironori Yoshino Yoshikazu Kuwahara Hiro Sakuraba Yoichiro Hosokawa |
| author_facet | Shingo Terashima Hironori Yoshino Yoshikazu Kuwahara Hiro Sakuraba Yoichiro Hosokawa |
| author_sort | Shingo Terashima |
| collection | DOAJ |
| description | We demonstrated that low dose pulsed radiation (0.25 Gy) at a high-dose-rate, even for very short intervals (10 s), decreases cell survival to a greater extent than single exposure to a similar total dose and dose rate. The objective of this study was to clarify whether high-dose-rate pulsed radiation is effective against SAS-R, a clinically relevant radioresistant cell line. Cell survival following high-dose-rate pulsed radiation was evaluated via a colony assay. Flow cytometry was utilized to evaluate γH2AX, a molecular marker of DNA double-strand breaks and delayed reactive oxygen species (ROS) associated with radiation-induced apoptosis. Increased cytotoxicity was observed in SAS-R and parent SAS cells in response to high dose rate pulsed radiation compared to single dose, as determined by colony assays. Residual γH2AX in both cells subjected to high-dose-rate pulsed radiation showed a tendency to increase, with a significant increase observed in SAS cells at 72 h. In addition, high-dose-rate pulsed radiation increased delayed ROS more than the single exposure did. These results indicate that high-dose-rate pulsed radiation was associated with residual γH2AX and delayed ROS, and high-dose-rate pulsed radiation may be used as an effective radiotherapy procedure against radioresistant cells. |
| first_indexed | 2024-03-10T03:44:20Z |
| format | Article |
| id | doaj.art-dc4b0d96a4fb4222a9b94c5d33734843 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T03:44:20Z |
| publishDate | 2021-11-01 |
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| record_format | Article |
| series | Life |
| spelling | doaj.art-dc4b0d96a4fb4222a9b94c5d337348432023-11-23T09:13:24ZengMDPI AGLife2075-17292021-11-011112129510.3390/life11121295The Effect of High-Dose-Rate Pulsed Radiation on the Survival of Clinically Relevant Radioresistant CellsShingo Terashima0Hironori Yoshino1Yoshikazu Kuwahara2Hiro Sakuraba3Yoichiro Hosokawa4Department of Radiation Science, Hirosaki University Graduate School of Health Sciences, Hirosaki 036-8564, JapanDepartment of Radiation Science, Hirosaki University Graduate School of Health Sciences, Hirosaki 036-8564, JapanDepartment of Radiation Biology and Medicine, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai 983-8536, JapanDepartment of Radiology, Aomori City Hospital, Aomori 030-0821, JapanDepartment of Radiation Science, Hirosaki University Graduate School of Health Sciences, Hirosaki 036-8564, JapanWe demonstrated that low dose pulsed radiation (0.25 Gy) at a high-dose-rate, even for very short intervals (10 s), decreases cell survival to a greater extent than single exposure to a similar total dose and dose rate. The objective of this study was to clarify whether high-dose-rate pulsed radiation is effective against SAS-R, a clinically relevant radioresistant cell line. Cell survival following high-dose-rate pulsed radiation was evaluated via a colony assay. Flow cytometry was utilized to evaluate γH2AX, a molecular marker of DNA double-strand breaks and delayed reactive oxygen species (ROS) associated with radiation-induced apoptosis. Increased cytotoxicity was observed in SAS-R and parent SAS cells in response to high dose rate pulsed radiation compared to single dose, as determined by colony assays. Residual γH2AX in both cells subjected to high-dose-rate pulsed radiation showed a tendency to increase, with a significant increase observed in SAS cells at 72 h. In addition, high-dose-rate pulsed radiation increased delayed ROS more than the single exposure did. These results indicate that high-dose-rate pulsed radiation was associated with residual γH2AX and delayed ROS, and high-dose-rate pulsed radiation may be used as an effective radiotherapy procedure against radioresistant cells.https://www.mdpi.com/2075-1729/11/12/1295radiation therapyclinically relevant radioresistant cellsγH2AXpulsed radiationROS |
| spellingShingle | Shingo Terashima Hironori Yoshino Yoshikazu Kuwahara Hiro Sakuraba Yoichiro Hosokawa The Effect of High-Dose-Rate Pulsed Radiation on the Survival of Clinically Relevant Radioresistant Cells Life radiation therapy clinically relevant radioresistant cells γH2AX pulsed radiation ROS |
| title | The Effect of High-Dose-Rate Pulsed Radiation on the Survival of Clinically Relevant Radioresistant Cells |
| title_full | The Effect of High-Dose-Rate Pulsed Radiation on the Survival of Clinically Relevant Radioresistant Cells |
| title_fullStr | The Effect of High-Dose-Rate Pulsed Radiation on the Survival of Clinically Relevant Radioresistant Cells |
| title_full_unstemmed | The Effect of High-Dose-Rate Pulsed Radiation on the Survival of Clinically Relevant Radioresistant Cells |
| title_short | The Effect of High-Dose-Rate Pulsed Radiation on the Survival of Clinically Relevant Radioresistant Cells |
| title_sort | effect of high dose rate pulsed radiation on the survival of clinically relevant radioresistant cells |
| topic | radiation therapy clinically relevant radioresistant cells γH2AX pulsed radiation ROS |
| url | https://www.mdpi.com/2075-1729/11/12/1295 |
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