Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in <i>Dracaena cambodiana</i> Response to Ultraviolet-B Radiation
Ultraviolet-B (UV-B) radiation is a major abiotic stress that dragon trees are exposed to during their growth and development; however, it is also an environmental signal perceived by plants that affects the flavonoid pathway. Previous studies have demonstrated that amounts of flavonoids are contain...
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MDPI AG
2023-05-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/14/5/979 |
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| author | Yue-E Liang Hao Zhang Jiahong Zhu Hao Wang Wenli Mei Bei Jiang Xupo Ding Haofu Dai |
| author_facet | Yue-E Liang Hao Zhang Jiahong Zhu Hao Wang Wenli Mei Bei Jiang Xupo Ding Haofu Dai |
| author_sort | Yue-E Liang |
| collection | DOAJ |
| description | Ultraviolet-B (UV-B) radiation is a major abiotic stress that dragon trees are exposed to during their growth and development; however, it is also an environmental signal perceived by plants that affects the flavonoid pathway. Previous studies have demonstrated that amounts of flavonoids are contained in dragon tree resin, otherwise known as dragon’s blood. However, the traits and mechanism involved in the UV-B-mediated increase in flavonoids in dragon trees are still unknown. Here, we studied the response of <i>Dracaena cambodiana</i> under full solar UV-B radiation. The results showed that the contents of total flavonoids in <i>D. cambodiana</i> significantly increased after UV-B radiation exposure. Then, the transcriptome was used for determining the interactive mechanism of flavonoid accumulation and UV-B stress. Differential expression analyses identified 34 differentially expressed genes (DEGs) involved in flavonoid synthesis; specifically, 24 of the identified DEGs were significantly up-regulated after UV-B radiation exposure. In addition, 57 DEGs involved in Ca<sup>2+</sup>/kinase sensors, 58 DEGs involved in ROS scavenging and the plant hormone pathway, and 116 DEGs transcription factors in 5 families were further identified and analyzed. Finally, we deduced the potential mechanism of UV-B-promoting flavonoid formation to neutralize ROS damage derived from UV-B radiation in <i>D. cambodiana</i> based on the gene co-expression network and previous studies from other plants. Considering that wild dragon tree populations are currently highly threatened by anthropogenic and natural stressors, the interactive studies between <i>D. cambodiana</i> plants and UV-B radiation provide valuable information toward understanding the mechanism of dragon’s blood formation and help us reveal the evolution of <i>D. cambodiana</i>, with the eventual goal of aiding in the global conservation of this precious biological resource. |
| first_indexed | 2024-03-11T03:44:04Z |
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| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-11T03:44:04Z |
| publishDate | 2023-05-01 |
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| series | Forests |
| spelling | doaj.art-dc4b0efddffc48ffbd6aa3b3824176862023-11-18T01:24:32ZengMDPI AGForests1999-49072023-05-0114597910.3390/f14050979Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in <i>Dracaena cambodiana</i> Response to Ultraviolet-B RadiationYue-E Liang0Hao Zhang1Jiahong Zhu2Hao Wang3Wenli Mei4Bei Jiang5Xupo Ding6Haofu Dai7College of Pharmacy, Dali University, Dali 671003, ChinaKey Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaKey Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaKey Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaKey Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaCollege of Pharmacy, Dali University, Dali 671003, ChinaKey Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaKey Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaUltraviolet-B (UV-B) radiation is a major abiotic stress that dragon trees are exposed to during their growth and development; however, it is also an environmental signal perceived by plants that affects the flavonoid pathway. Previous studies have demonstrated that amounts of flavonoids are contained in dragon tree resin, otherwise known as dragon’s blood. However, the traits and mechanism involved in the UV-B-mediated increase in flavonoids in dragon trees are still unknown. Here, we studied the response of <i>Dracaena cambodiana</i> under full solar UV-B radiation. The results showed that the contents of total flavonoids in <i>D. cambodiana</i> significantly increased after UV-B radiation exposure. Then, the transcriptome was used for determining the interactive mechanism of flavonoid accumulation and UV-B stress. Differential expression analyses identified 34 differentially expressed genes (DEGs) involved in flavonoid synthesis; specifically, 24 of the identified DEGs were significantly up-regulated after UV-B radiation exposure. In addition, 57 DEGs involved in Ca<sup>2+</sup>/kinase sensors, 58 DEGs involved in ROS scavenging and the plant hormone pathway, and 116 DEGs transcription factors in 5 families were further identified and analyzed. Finally, we deduced the potential mechanism of UV-B-promoting flavonoid formation to neutralize ROS damage derived from UV-B radiation in <i>D. cambodiana</i> based on the gene co-expression network and previous studies from other plants. Considering that wild dragon tree populations are currently highly threatened by anthropogenic and natural stressors, the interactive studies between <i>D. cambodiana</i> plants and UV-B radiation provide valuable information toward understanding the mechanism of dragon’s blood formation and help us reveal the evolution of <i>D. cambodiana</i>, with the eventual goal of aiding in the global conservation of this precious biological resource.https://www.mdpi.com/1999-4907/14/5/979<i>Dracaena cambodiana</i>UV-BRNA-seqflavonoids metabolismco-expression networktranscription factors |
| spellingShingle | Yue-E Liang Hao Zhang Jiahong Zhu Hao Wang Wenli Mei Bei Jiang Xupo Ding Haofu Dai Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in <i>Dracaena cambodiana</i> Response to Ultraviolet-B Radiation Forests <i>Dracaena cambodiana</i> UV-B RNA-seq flavonoids metabolism co-expression network transcription factors |
| title | Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in <i>Dracaena cambodiana</i> Response to Ultraviolet-B Radiation |
| title_full | Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in <i>Dracaena cambodiana</i> Response to Ultraviolet-B Radiation |
| title_fullStr | Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in <i>Dracaena cambodiana</i> Response to Ultraviolet-B Radiation |
| title_full_unstemmed | Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in <i>Dracaena cambodiana</i> Response to Ultraviolet-B Radiation |
| title_short | Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in <i>Dracaena cambodiana</i> Response to Ultraviolet-B Radiation |
| title_sort | transcriptomic analysis reveals the involvement of flavonoids synthesis genes and transcription factors in i dracaena cambodiana i response to ultraviolet b radiation |
| topic | <i>Dracaena cambodiana</i> UV-B RNA-seq flavonoids metabolism co-expression network transcription factors |
| url | https://www.mdpi.com/1999-4907/14/5/979 |
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