Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines
Abstract Background Propolis is a natural bee product with a wide range of biological activities that are related to its chemical composition. The present study investigated the quantification of quercetin (Q) in Ardabil ethanol extract of propolis (AEEP), and then compared its anti-bacterial, anti-...
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-019-2677-3 |
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| author | Fariba Asgharpour Ali Akbar Moghadamnia Ebrahim Zabihi Sohrab Kazemi Amirmorteza Ebrahimzadeh Namvar Hemmat Gholinia Mina Motallebnejad Hamid Reza Nouri |
| author_facet | Fariba Asgharpour Ali Akbar Moghadamnia Ebrahim Zabihi Sohrab Kazemi Amirmorteza Ebrahimzadeh Namvar Hemmat Gholinia Mina Motallebnejad Hamid Reza Nouri |
| author_sort | Fariba Asgharpour |
| collection | DOAJ |
| description | Abstract Background Propolis is a natural bee product with a wide range of biological activities that are related to its chemical composition. The present study investigated the quantification of quercetin (Q) in Ardabil ethanol extract of propolis (AEEP), and then compared its anti-bacterial, anti- biofilm and cytotoxic effects on cancer and normal cell lines. Method In the present study, the chemical composition of AEEP was determined through the high-performance liquid chromatography (HPLC). The AEEP and its main component, quercetin (Q), were evaluated in vitro against 57 oral streptococci by a broth micro-dilution method. The biofilm formation was assessed through the crystal violet staining and MTT assays. The impact of AEEP and Q anti-proliferative effect were evaluated on the fibroblast as normal and cancer cell lines (KB and A431). Results The Q concentration in the composition of AEEP was 6.9% of all its components. The findings indicated that the AEEP and Q were efficient against the cariogenic bacteria and were able to inhibit the S.mutans biofilm adherence at a sub-MIC concentration. Moreover, electron micrographs indicated the inhibition of biofilms compared to control biofilms. In addition, the AEEP and Q indicated a dose-dependent cytotoxic effect on A431 and KB cell lines. On the contrary, they had no cytotoxic effect on fibroblast cells. Conclusion The results indicated that the synergistic impact of main components of AEEP was related to the inhibition of the cancer cell proliferation, cariogenic bacteria and oral biofilm formation. It may play a promising role in the complementary medicine and, it is suggested to be used as food additives. |
| first_indexed | 2024-12-12T13:05:54Z |
| format | Article |
| id | doaj.art-dc5271d7559440a085e668602d6f16ef |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-12T13:05:54Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-dc5271d7559440a085e668602d6f16ef2022-12-22T00:23:39ZengBMCBMC Complementary and Alternative Medicine1472-68822019-10-011911810.1186/s12906-019-2677-3Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell linesFariba Asgharpour0Ali Akbar Moghadamnia1Ebrahim Zabihi2Sohrab Kazemi3Amirmorteza Ebrahimzadeh Namvar4Hemmat Gholinia5Mina Motallebnejad6Hamid Reza Nouri7Student Research Committee, Babol University of Medical SciencesDepartment of Pharmacology, Faculty of Medicine, Babol University of Medical SciencesCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical SciencesCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical SciencesDepartment of Microbiology, Faculty of Medicine, Babol University of Medical SciencesClinical Research Development Unit of Ayatollah Rohani Hospital, Babol University of Medical SciencesOral Health Research Center, Health Research Institute, Babol University of Medical SciencesCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical SciencesAbstract Background Propolis is a natural bee product with a wide range of biological activities that are related to its chemical composition. The present study investigated the quantification of quercetin (Q) in Ardabil ethanol extract of propolis (AEEP), and then compared its anti-bacterial, anti- biofilm and cytotoxic effects on cancer and normal cell lines. Method In the present study, the chemical composition of AEEP was determined through the high-performance liquid chromatography (HPLC). The AEEP and its main component, quercetin (Q), were evaluated in vitro against 57 oral streptococci by a broth micro-dilution method. The biofilm formation was assessed through the crystal violet staining and MTT assays. The impact of AEEP and Q anti-proliferative effect were evaluated on the fibroblast as normal and cancer cell lines (KB and A431). Results The Q concentration in the composition of AEEP was 6.9% of all its components. The findings indicated that the AEEP and Q were efficient against the cariogenic bacteria and were able to inhibit the S.mutans biofilm adherence at a sub-MIC concentration. Moreover, electron micrographs indicated the inhibition of biofilms compared to control biofilms. In addition, the AEEP and Q indicated a dose-dependent cytotoxic effect on A431 and KB cell lines. On the contrary, they had no cytotoxic effect on fibroblast cells. Conclusion The results indicated that the synergistic impact of main components of AEEP was related to the inhibition of the cancer cell proliferation, cariogenic bacteria and oral biofilm formation. It may play a promising role in the complementary medicine and, it is suggested to be used as food additives.http://link.springer.com/article/10.1186/s12906-019-2677-3Anti-bacterialAnti-biofilmsPropolisQuercetin |
| spellingShingle | Fariba Asgharpour Ali Akbar Moghadamnia Ebrahim Zabihi Sohrab Kazemi Amirmorteza Ebrahimzadeh Namvar Hemmat Gholinia Mina Motallebnejad Hamid Reza Nouri Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines BMC Complementary and Alternative Medicine Anti-bacterial Anti-biofilms Propolis Quercetin |
| title | Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines |
| title_full | Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines |
| title_fullStr | Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines |
| title_full_unstemmed | Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines |
| title_short | Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines |
| title_sort | iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines |
| topic | Anti-bacterial Anti-biofilms Propolis Quercetin |
| url | http://link.springer.com/article/10.1186/s12906-019-2677-3 |
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