Transcription induces context-dependent remodeling of chromatin architecture during differentiation.
Metazoan chromosomes are organized into discrete spatial domains (TADs), believed to contribute to the regulation of transcriptional programs. Despite extensive correlation between domain organization and gene activity, a direct mechanistic link is unclear, with perturbation studies often showing li...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-12-01
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| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3002424&type=printable |
| _version_ | 1797377798112804864 |
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| author | Sanjay Chahar Yousra Ben Zouari Hossein Salari Dominique Kobi Manon Maroquenne Cathie Erb Anne M Molitor Audrey Mossler Nezih Karasu Daniel Jost Tom Sexton |
| author_facet | Sanjay Chahar Yousra Ben Zouari Hossein Salari Dominique Kobi Manon Maroquenne Cathie Erb Anne M Molitor Audrey Mossler Nezih Karasu Daniel Jost Tom Sexton |
| author_sort | Sanjay Chahar |
| collection | DOAJ |
| description | Metazoan chromosomes are organized into discrete spatial domains (TADs), believed to contribute to the regulation of transcriptional programs. Despite extensive correlation between domain organization and gene activity, a direct mechanistic link is unclear, with perturbation studies often showing little effect. To follow chromatin architecture changes during development, we used Capture Hi-C to interrogate the domains around key differentially expressed genes during mouse thymocyte maturation, uncovering specific remodeling events. Notably, one TAD boundary was broadened to accommodate RNA polymerase elongation past the border, and subdomains were formed around some activated genes without changes in CTCF binding. The ectopic induction of some genes was sufficient to recapitulate domain formation in embryonic stem cells, providing strong evidence that transcription can directly remodel chromatin structure. These results suggest that transcriptional processes drive complex chromosome folding patterns that can be important in certain genomic contexts. |
| first_indexed | 2024-03-08T19:58:38Z |
| format | Article |
| id | doaj.art-dc52a925b29148809d0ed2d6152f8d00 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-03-08T19:58:38Z |
| publishDate | 2023-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-dc52a925b29148809d0ed2d6152f8d002023-12-24T05:31:22ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852023-12-012112e300242410.1371/journal.pbio.3002424Transcription induces context-dependent remodeling of chromatin architecture during differentiation.Sanjay ChaharYousra Ben ZouariHossein SalariDominique KobiManon MaroquenneCathie ErbAnne M MolitorAudrey MosslerNezih KarasuDaniel JostTom SextonMetazoan chromosomes are organized into discrete spatial domains (TADs), believed to contribute to the regulation of transcriptional programs. Despite extensive correlation between domain organization and gene activity, a direct mechanistic link is unclear, with perturbation studies often showing little effect. To follow chromatin architecture changes during development, we used Capture Hi-C to interrogate the domains around key differentially expressed genes during mouse thymocyte maturation, uncovering specific remodeling events. Notably, one TAD boundary was broadened to accommodate RNA polymerase elongation past the border, and subdomains were formed around some activated genes without changes in CTCF binding. The ectopic induction of some genes was sufficient to recapitulate domain formation in embryonic stem cells, providing strong evidence that transcription can directly remodel chromatin structure. These results suggest that transcriptional processes drive complex chromosome folding patterns that can be important in certain genomic contexts.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3002424&type=printable |
| spellingShingle | Sanjay Chahar Yousra Ben Zouari Hossein Salari Dominique Kobi Manon Maroquenne Cathie Erb Anne M Molitor Audrey Mossler Nezih Karasu Daniel Jost Tom Sexton Transcription induces context-dependent remodeling of chromatin architecture during differentiation. PLoS Biology |
| title | Transcription induces context-dependent remodeling of chromatin architecture during differentiation. |
| title_full | Transcription induces context-dependent remodeling of chromatin architecture during differentiation. |
| title_fullStr | Transcription induces context-dependent remodeling of chromatin architecture during differentiation. |
| title_full_unstemmed | Transcription induces context-dependent remodeling of chromatin architecture during differentiation. |
| title_short | Transcription induces context-dependent remodeling of chromatin architecture during differentiation. |
| title_sort | transcription induces context dependent remodeling of chromatin architecture during differentiation |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3002424&type=printable |
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