Time-Resolved Fluorescence Spectroscopy Study of Energy Transfer Dynamics in Phycobilisomes from Cyanobacteria <i>Thermosynechococcus vulcanus NIES 2134</i> and <i>Synechocystis</i> sp. <i>PCC 680</i><i>3</i>
As the largest light-harvesting complex in cyanobacteria, phycobilisomes (PBSs) show high efficiency and a high rate of energy transfer, owing to an elegant antenna-like assembly. To understand the structural influence on the dynamic process of the energy transfer in PBSs, two cyanobacterium species...
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2021-10-01
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| author | Mingyuan Xie Wenjun Li Chao Xiao Zhanghe Zhen Jianfei Ma Hanzhi Lin Song Qin Fuli Zhao |
| author_facet | Mingyuan Xie Wenjun Li Chao Xiao Zhanghe Zhen Jianfei Ma Hanzhi Lin Song Qin Fuli Zhao |
| author_sort | Mingyuan Xie |
| collection | DOAJ |
| description | As the largest light-harvesting complex in cyanobacteria, phycobilisomes (PBSs) show high efficiency and a high rate of energy transfer, owing to an elegant antenna-like assembly. To understand the structural influence on the dynamic process of the energy transfer in PBSs, two cyanobacterium species <i>Thermosynechococcus vulcanus NIES 2134</i> (<i>T. 2134</i>) and <i>Synechocystis sp. PCC 6803</i> (<i>S. 6803</i>) with different rod–core-linked assemblies were chosen for this study. The dynamic process of the energy transfer in both PBSs was investigated through time-resolved fluorescence spectroscopy (TRFS) with a time resolution of sub-picosecond. Via the fluorescence decay curves deconvolution, the pathways and related rates of the excitation energy transfer (EET) were determined. Three time components, i.e., 10, 80, and 1250 ps, were identified in the EET in the PBSs of <i>T. 2134</i> and three, i.e., 9, 115, and 1680 ps, in the EET in the PBSs of <i>S. 6803</i>. In addition, a comparison of the dynamic process of the energy transfer between the two cyanobacteria revealed how the PBS assembly affects the energy transfer in PBSs. The findings will provide insight into future time-resolved crystallography. |
| first_indexed | 2024-03-10T06:38:59Z |
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| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-03-10T06:38:59Z |
| publishDate | 2021-10-01 |
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| series | Crystals |
| spelling | doaj.art-dc5561cda7384ecabe8022af29f476542023-11-22T17:53:43ZengMDPI AGCrystals2073-43522021-10-011110123310.3390/cryst11101233Time-Resolved Fluorescence Spectroscopy Study of Energy Transfer Dynamics in Phycobilisomes from Cyanobacteria <i>Thermosynechococcus vulcanus NIES 2134</i> and <i>Synechocystis</i> sp. <i>PCC 680</i><i>3</i>Mingyuan Xie0Wenjun Li1Chao Xiao2Zhanghe Zhen3Jianfei Ma4Hanzhi Lin5Song Qin6Fuli Zhao7State Key Laboratory of Optoelectronic Materials and Technologies, School of Physics, Sun Yat-Sen University, Guangzhou 510275, ChinaYantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaState Key Laboratory of Optoelectronic Materials and Technologies, School of Physics, Sun Yat-Sen University, Guangzhou 510275, ChinaYantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaState Key Laboratory of Membrane Biology, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, ChinaInstitute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD 21202, USAYantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaState Key Laboratory of Optoelectronic Materials and Technologies, School of Physics, Sun Yat-Sen University, Guangzhou 510275, ChinaAs the largest light-harvesting complex in cyanobacteria, phycobilisomes (PBSs) show high efficiency and a high rate of energy transfer, owing to an elegant antenna-like assembly. To understand the structural influence on the dynamic process of the energy transfer in PBSs, two cyanobacterium species <i>Thermosynechococcus vulcanus NIES 2134</i> (<i>T. 2134</i>) and <i>Synechocystis sp. PCC 6803</i> (<i>S. 6803</i>) with different rod–core-linked assemblies were chosen for this study. The dynamic process of the energy transfer in both PBSs was investigated through time-resolved fluorescence spectroscopy (TRFS) with a time resolution of sub-picosecond. Via the fluorescence decay curves deconvolution, the pathways and related rates of the excitation energy transfer (EET) were determined. Three time components, i.e., 10, 80, and 1250 ps, were identified in the EET in the PBSs of <i>T. 2134</i> and three, i.e., 9, 115, and 1680 ps, in the EET in the PBSs of <i>S. 6803</i>. In addition, a comparison of the dynamic process of the energy transfer between the two cyanobacteria revealed how the PBS assembly affects the energy transfer in PBSs. The findings will provide insight into future time-resolved crystallography.https://www.mdpi.com/2073-4352/11/10/1233phycobilisomescyanobacteriatime-resolved fluorescence spectroscopyenergy transfer |
| spellingShingle | Mingyuan Xie Wenjun Li Chao Xiao Zhanghe Zhen Jianfei Ma Hanzhi Lin Song Qin Fuli Zhao Time-Resolved Fluorescence Spectroscopy Study of Energy Transfer Dynamics in Phycobilisomes from Cyanobacteria <i>Thermosynechococcus vulcanus NIES 2134</i> and <i>Synechocystis</i> sp. <i>PCC 680</i><i>3</i> Crystals phycobilisomes cyanobacteria time-resolved fluorescence spectroscopy energy transfer |
| title | Time-Resolved Fluorescence Spectroscopy Study of Energy Transfer Dynamics in Phycobilisomes from Cyanobacteria <i>Thermosynechococcus vulcanus NIES 2134</i> and <i>Synechocystis</i> sp. <i>PCC 680</i><i>3</i> |
| title_full | Time-Resolved Fluorescence Spectroscopy Study of Energy Transfer Dynamics in Phycobilisomes from Cyanobacteria <i>Thermosynechococcus vulcanus NIES 2134</i> and <i>Synechocystis</i> sp. <i>PCC 680</i><i>3</i> |
| title_fullStr | Time-Resolved Fluorescence Spectroscopy Study of Energy Transfer Dynamics in Phycobilisomes from Cyanobacteria <i>Thermosynechococcus vulcanus NIES 2134</i> and <i>Synechocystis</i> sp. <i>PCC 680</i><i>3</i> |
| title_full_unstemmed | Time-Resolved Fluorescence Spectroscopy Study of Energy Transfer Dynamics in Phycobilisomes from Cyanobacteria <i>Thermosynechococcus vulcanus NIES 2134</i> and <i>Synechocystis</i> sp. <i>PCC 680</i><i>3</i> |
| title_short | Time-Resolved Fluorescence Spectroscopy Study of Energy Transfer Dynamics in Phycobilisomes from Cyanobacteria <i>Thermosynechococcus vulcanus NIES 2134</i> and <i>Synechocystis</i> sp. <i>PCC 680</i><i>3</i> |
| title_sort | time resolved fluorescence spectroscopy study of energy transfer dynamics in phycobilisomes from cyanobacteria i thermosynechococcus vulcanus nies 2134 i and i synechocystis i sp i pcc 680 i i 3 i |
| topic | phycobilisomes cyanobacteria time-resolved fluorescence spectroscopy energy transfer |
| url | https://www.mdpi.com/2073-4352/11/10/1233 |
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