A New, Non-Invasive Methodology for the Molecular Identification of Adult Sarcophagidae from Collections
Correct species identification is the cornerstone of all scientific studies that involve insects. Alongside traditional morphological identification techniques, molecular identification based on the characterization and analysis of specific mitochondrial or nuclear gene regions is becoming commonpla...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/14/7/635 |
| _version_ | 1797588937442590720 |
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| author | Giorgia Giordani Daniel Whitmore Stefano Vanin |
| author_facet | Giorgia Giordani Daniel Whitmore Stefano Vanin |
| author_sort | Giorgia Giordani |
| collection | DOAJ |
| description | Correct species identification is the cornerstone of all scientific studies that involve insects. Alongside traditional morphological identification techniques, molecular identification based on the characterization and analysis of specific mitochondrial or nuclear gene regions is becoming commonplace. Despite the good results that can be achieved, DNA extraction usually involves invasive techniques that lead to the partial or total destruction of specimens. In this work, a non-invasive DNA extraction technique is described. The technique was tested on the abdomens of dry-preserved Sarcophagidae (Diptera) specimens collected between 1889 and 2015. This allowed for the correct identification of species without impairing diagnostic morphological structures useful for further studies. |
| first_indexed | 2024-03-11T00:59:09Z |
| format | Article |
| id | doaj.art-dc5781982cd24f48859b40cc49790a65 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T00:59:09Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-dc5781982cd24f48859b40cc49790a652023-11-18T19:49:19ZengMDPI AGInsects2075-44502023-07-0114763510.3390/insects14070635A New, Non-Invasive Methodology for the Molecular Identification of Adult Sarcophagidae from CollectionsGiorgia Giordani0Daniel Whitmore1Stefano Vanin2Dipartimento di Farmacia e Biotecnologie (FABIT), Alma Mater Studiorum Università di Bologna, 40126 Bologna, ItalyStaatliches Museum für Naturkunde Stuttgart, 70191 Stuttgart, GermanyDipartimento di Scienze della Terra dell’Ambiente e della Vita (DISTAV), Università di Genova, 16132 Genova, ItalyCorrect species identification is the cornerstone of all scientific studies that involve insects. Alongside traditional morphological identification techniques, molecular identification based on the characterization and analysis of specific mitochondrial or nuclear gene regions is becoming commonplace. Despite the good results that can be achieved, DNA extraction usually involves invasive techniques that lead to the partial or total destruction of specimens. In this work, a non-invasive DNA extraction technique is described. The technique was tested on the abdomens of dry-preserved Sarcophagidae (Diptera) specimens collected between 1889 and 2015. This allowed for the correct identification of species without impairing diagnostic morphological structures useful for further studies.https://www.mdpi.com/2075-4450/14/7/635identificationCOI<i>Sarcophaga</i>barcodingspecimen preservationDNA |
| spellingShingle | Giorgia Giordani Daniel Whitmore Stefano Vanin A New, Non-Invasive Methodology for the Molecular Identification of Adult Sarcophagidae from Collections Insects identification COI <i>Sarcophaga</i> barcoding specimen preservation DNA |
| title | A New, Non-Invasive Methodology for the Molecular Identification of Adult Sarcophagidae from Collections |
| title_full | A New, Non-Invasive Methodology for the Molecular Identification of Adult Sarcophagidae from Collections |
| title_fullStr | A New, Non-Invasive Methodology for the Molecular Identification of Adult Sarcophagidae from Collections |
| title_full_unstemmed | A New, Non-Invasive Methodology for the Molecular Identification of Adult Sarcophagidae from Collections |
| title_short | A New, Non-Invasive Methodology for the Molecular Identification of Adult Sarcophagidae from Collections |
| title_sort | new non invasive methodology for the molecular identification of adult sarcophagidae from collections |
| topic | identification COI <i>Sarcophaga</i> barcoding specimen preservation DNA |
| url | https://www.mdpi.com/2075-4450/14/7/635 |
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