An internal transcribed spacer 2 gene fragment, as a suitable phylogenetic marker in Hyalomma and Rhipicephalus (Acari: Rhipicephalinae) species
Hyalomma and Rhipicephalus (Acari: Ixodidae: Rhipicephalinae) are the most important ectoparasites and biological vector of pathogen agents. The taxonomy of some Hyalomma and Rhipicephalus species is more debatable. The traditional taxonomy of ticks have been revolutionized using polymerase chain re...
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2016-05-01
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| Series: | Iranian Journal of Animal Biosystematics |
| Subjects: | |
| Online Access: | https://ijab.um.ac.ir/article_28555_0e143743e56d6050c686d7e557f934c9.pdf |
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| author | Zakkyeh Telmadarraiy Mohammad Nasrabadi Mohammad Mehdi Sedaghat Asadollah Hosseini-Chegeni Reza Hosseini Saedeh Sadat Hashemi-Aghdam S. Gholami |
| author_facet | Zakkyeh Telmadarraiy Mohammad Nasrabadi Mohammad Mehdi Sedaghat Asadollah Hosseini-Chegeni Reza Hosseini Saedeh Sadat Hashemi-Aghdam S. Gholami |
| author_sort | Zakkyeh Telmadarraiy |
| collection | DOAJ |
| description | Hyalomma and Rhipicephalus (Acari: Ixodidae: Rhipicephalinae) are the most important ectoparasites and biological vector of pathogen agents. The taxonomy of some Hyalomma and Rhipicephalus species is more debatable. The traditional taxonomy of ticks have been revolutionized using polymerase chain reaction. The specificity, efficiency and accuracy of PCR is highly dependent on the oligonucleotide primers. In this study, five primers designed for amplification of a fragment ITS2 so-called TAH-1, TAH-2, TRH (for Hyalomma) and TAH-3, TAH-4 (for Rhipicephalus). Successfully, 24 and 29 ITS2 sample was sequenced using these primers. As well as, an ITS2 phylogenetic tree were constructed using BEAST basis on the Bayesian Inference (BI) method. The most Hyalomma and Rhipicephalus species well differentiated using ITS2 fragment since the designed primer fail to amplify gene target in few cases. |
| first_indexed | 2024-04-11T21:05:23Z |
| format | Article |
| id | doaj.art-dc57d261c51441e99e9bb5888225ce9f |
| institution | Directory Open Access Journal |
| issn | 1735-434X 2423-4222 |
| language | English |
| last_indexed | 2024-04-11T21:05:23Z |
| publishDate | 2016-05-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | Iranian Journal of Animal Biosystematics |
| spelling | doaj.art-dc57d261c51441e99e9bb5888225ce9f2022-12-22T04:03:21ZengFerdowsi University of MashhadIranian Journal of Animal Biosystematics1735-434X2423-42222016-05-0112210.22067/ijab.v12i2.4941528555An internal transcribed spacer 2 gene fragment, as a suitable phylogenetic marker in Hyalomma and Rhipicephalus (Acari: Rhipicephalinae) speciesZakkyeh Telmadarraiy0Mohammad Nasrabadi1Mohammad Mehdi Sedaghat2Asadollah Hosseini-Chegeni3Reza Hosseini4Saedeh Sadat Hashemi-Aghdam5S. Gholami6Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Plant Protection, Faculty of Agriculture, University of Lorestan, Lorestan, IranDepartment of Plant Protection, Faculty of Agriculture, University of Guilan, Guilan, IranDeptartment of Biology, Damghan Branch, Islamic Azad University, Damghan, IranDepartment of Plant Protection, Faculty of Agriculture, University of Guilan, Guilan, IranHyalomma and Rhipicephalus (Acari: Ixodidae: Rhipicephalinae) are the most important ectoparasites and biological vector of pathogen agents. The taxonomy of some Hyalomma and Rhipicephalus species is more debatable. The traditional taxonomy of ticks have been revolutionized using polymerase chain reaction. The specificity, efficiency and accuracy of PCR is highly dependent on the oligonucleotide primers. In this study, five primers designed for amplification of a fragment ITS2 so-called TAH-1, TAH-2, TRH (for Hyalomma) and TAH-3, TAH-4 (for Rhipicephalus). Successfully, 24 and 29 ITS2 sample was sequenced using these primers. As well as, an ITS2 phylogenetic tree were constructed using BEAST basis on the Bayesian Inference (BI) method. The most Hyalomma and Rhipicephalus species well differentiated using ITS2 fragment since the designed primer fail to amplify gene target in few cases.https://ijab.um.ac.ir/article_28555_0e143743e56d6050c686d7e557f934c9.pdfpcrprimerits2hyalommarhipicephalusphylogenetic relationship |
| spellingShingle | Zakkyeh Telmadarraiy Mohammad Nasrabadi Mohammad Mehdi Sedaghat Asadollah Hosseini-Chegeni Reza Hosseini Saedeh Sadat Hashemi-Aghdam S. Gholami An internal transcribed spacer 2 gene fragment, as a suitable phylogenetic marker in Hyalomma and Rhipicephalus (Acari: Rhipicephalinae) species Iranian Journal of Animal Biosystematics pcr primer its2 hyalomma rhipicephalus phylogenetic relationship |
| title | An internal transcribed spacer 2 gene fragment, as a suitable phylogenetic marker in Hyalomma and Rhipicephalus (Acari: Rhipicephalinae) species |
| title_full | An internal transcribed spacer 2 gene fragment, as a suitable phylogenetic marker in Hyalomma and Rhipicephalus (Acari: Rhipicephalinae) species |
| title_fullStr | An internal transcribed spacer 2 gene fragment, as a suitable phylogenetic marker in Hyalomma and Rhipicephalus (Acari: Rhipicephalinae) species |
| title_full_unstemmed | An internal transcribed spacer 2 gene fragment, as a suitable phylogenetic marker in Hyalomma and Rhipicephalus (Acari: Rhipicephalinae) species |
| title_short | An internal transcribed spacer 2 gene fragment, as a suitable phylogenetic marker in Hyalomma and Rhipicephalus (Acari: Rhipicephalinae) species |
| title_sort | internal transcribed spacer 2 gene fragment as a suitable phylogenetic marker in hyalomma and rhipicephalus acari rhipicephalinae species |
| topic | pcr primer its2 hyalomma rhipicephalus phylogenetic relationship |
| url | https://ijab.um.ac.ir/article_28555_0e143743e56d6050c686d7e557f934c9.pdf |
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