Synthesis and Characterization of Novel Calcium-Silicate Nanobioceramics with Magnesium: Effect of Heat Treatment on Biological, Physical and Chemical Properties
Glass-ceramic nanopowder with a composition of 55SiO<sub>2</sub>-35CaO-10MgO (mol %) was synthesized by the sol–gel method and was heat treated at three temperatures (T1 = 835 °C, T2 = 1000 °C, T3 = 1100 °C) in order to obtain different materials (C1, C2, C3, respectively) varying in cry...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Ceramics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2571-6131/4/4/45 |
| _version_ | 1797505923558670336 |
|---|---|
| author | Konstantina Kazeli Ioannis Tsamesidis Anna Theocharidou Lamprini Malletzidou Jonathan Rhoades Georgia K. Pouroutzidou Eleni Likotrafiti Konstantinos Chrissafis Theodoros Lialiaris Lambrini Papadopoulou Eleana Kontonasaki Evgenia Lymperaki |
| author_facet | Konstantina Kazeli Ioannis Tsamesidis Anna Theocharidou Lamprini Malletzidou Jonathan Rhoades Georgia K. Pouroutzidou Eleni Likotrafiti Konstantinos Chrissafis Theodoros Lialiaris Lambrini Papadopoulou Eleana Kontonasaki Evgenia Lymperaki |
| author_sort | Konstantina Kazeli |
| collection | DOAJ |
| description | Glass-ceramic nanopowder with a composition of 55SiO<sub>2</sub>-35CaO-10MgO (mol %) was synthesized by the sol–gel method and was heat treated at three temperatures (T1 = 835 °C, T2 = 1000 °C, T3 = 1100 °C) in order to obtain different materials (C1, C2, C3, respectively) varying in crystal structure. Bioactivity and oxidative stress were evaluated in simulated body fluid (SBF) for various time periods (up to 10 days). The structure of the synthesized materials and their apatite-forming ability were investigated by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy and Energy Dispersive Spectroscopy (SEM/EDS). The antibacterial properties of the synthesized materials were evaluated against three Gram-positive and four Gram-negative bacterial strains and their biocompatibility was verified on a primary cell line of human gingival fibroblasts (HGFs) by the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) assay. The crystallization of the materials was increased by sintering temperature. Heat treatment did not inhibit the bioactive behavior of the materials as apatite formation started after 3 days in SBF. C2, C3 showed some indications of apatite forming even from the first day. Regarding cell viability, a variety of biological behaviors, concerning both dose and time points, was observed between the positive control and the tested materials by both the MTT assay and oxidative stress analysis. In conclusion, the nanobioceramic materials of this study possess a multitude of attractive physicochemical and biological properties that make them suitable candidates for bone regeneration applications, fillers in nanocomposite scaffolds, or as grafts in bone cavities and periodontal lesions. |
| first_indexed | 2024-03-10T04:25:15Z |
| format | Article |
| id | doaj.art-dc57f2a37d2747c5a09f38d43a12777a |
| institution | Directory Open Access Journal |
| issn | 2571-6131 |
| language | English |
| last_indexed | 2024-03-10T04:25:15Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Ceramics |
| spelling | doaj.art-dc57f2a37d2747c5a09f38d43a12777a2023-11-23T07:40:12ZengMDPI AGCeramics2571-61312021-11-014462865110.3390/ceramics4040045Synthesis and Characterization of Novel Calcium-Silicate Nanobioceramics with Magnesium: Effect of Heat Treatment on Biological, Physical and Chemical PropertiesKonstantina Kazeli0Ioannis Tsamesidis1Anna Theocharidou2Lamprini Malletzidou3Jonathan Rhoades4Georgia K. Pouroutzidou5Eleni Likotrafiti6Konstantinos Chrissafis7Theodoros Lialiaris8Lambrini Papadopoulou9Eleana Kontonasaki10Evgenia Lymperaki11School of Health Sciences, Faculty of Medicine Alexandoupoli, Democritus University of Thrace, GR-68100 Alexandroupolis, GreeceDepartment of Biomedical Sciences, International Hellenic University, GR-57400 Thessaloniki, GreeceDepartment of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceAdvanced Materials and Devices Laboratory, School of Physics, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Food Science and Technology, International Hellenic University, GR-57400 Thessaloniki, GreeceAdvanced Materials and Devices Laboratory, School of Physics, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Food Science and Technology, International Hellenic University, GR-57400 Thessaloniki, GreeceAdvanced Materials and Devices Laboratory, School of Physics, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceSchool of Health Sciences, Faculty of Medicine Alexandoupoli, Democritus University of Thrace, GR-68100 Alexandroupolis, GreeceSchool of Geology, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Biomedical Sciences, International Hellenic University, GR-57400 Thessaloniki, GreeceGlass-ceramic nanopowder with a composition of 55SiO<sub>2</sub>-35CaO-10MgO (mol %) was synthesized by the sol–gel method and was heat treated at three temperatures (T1 = 835 °C, T2 = 1000 °C, T3 = 1100 °C) in order to obtain different materials (C1, C2, C3, respectively) varying in crystal structure. Bioactivity and oxidative stress were evaluated in simulated body fluid (SBF) for various time periods (up to 10 days). The structure of the synthesized materials and their apatite-forming ability were investigated by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy and Energy Dispersive Spectroscopy (SEM/EDS). The antibacterial properties of the synthesized materials were evaluated against three Gram-positive and four Gram-negative bacterial strains and their biocompatibility was verified on a primary cell line of human gingival fibroblasts (HGFs) by the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) assay. The crystallization of the materials was increased by sintering temperature. Heat treatment did not inhibit the bioactive behavior of the materials as apatite formation started after 3 days in SBF. C2, C3 showed some indications of apatite forming even from the first day. Regarding cell viability, a variety of biological behaviors, concerning both dose and time points, was observed between the positive control and the tested materials by both the MTT assay and oxidative stress analysis. In conclusion, the nanobioceramic materials of this study possess a multitude of attractive physicochemical and biological properties that make them suitable candidates for bone regeneration applications, fillers in nanocomposite scaffolds, or as grafts in bone cavities and periodontal lesions.https://www.mdpi.com/2571-6131/4/4/45silica-based nanoparticlesbioactivity assayoxidative stressantibacterial propertiesbiocompatibility assayhuman gingival fibroblast (HGFs) |
| spellingShingle | Konstantina Kazeli Ioannis Tsamesidis Anna Theocharidou Lamprini Malletzidou Jonathan Rhoades Georgia K. Pouroutzidou Eleni Likotrafiti Konstantinos Chrissafis Theodoros Lialiaris Lambrini Papadopoulou Eleana Kontonasaki Evgenia Lymperaki Synthesis and Characterization of Novel Calcium-Silicate Nanobioceramics with Magnesium: Effect of Heat Treatment on Biological, Physical and Chemical Properties Ceramics silica-based nanoparticles bioactivity assay oxidative stress antibacterial properties biocompatibility assay human gingival fibroblast (HGFs) |
| title | Synthesis and Characterization of Novel Calcium-Silicate Nanobioceramics with Magnesium: Effect of Heat Treatment on Biological, Physical and Chemical Properties |
| title_full | Synthesis and Characterization of Novel Calcium-Silicate Nanobioceramics with Magnesium: Effect of Heat Treatment on Biological, Physical and Chemical Properties |
| title_fullStr | Synthesis and Characterization of Novel Calcium-Silicate Nanobioceramics with Magnesium: Effect of Heat Treatment on Biological, Physical and Chemical Properties |
| title_full_unstemmed | Synthesis and Characterization of Novel Calcium-Silicate Nanobioceramics with Magnesium: Effect of Heat Treatment on Biological, Physical and Chemical Properties |
| title_short | Synthesis and Characterization of Novel Calcium-Silicate Nanobioceramics with Magnesium: Effect of Heat Treatment on Biological, Physical and Chemical Properties |
| title_sort | synthesis and characterization of novel calcium silicate nanobioceramics with magnesium effect of heat treatment on biological physical and chemical properties |
| topic | silica-based nanoparticles bioactivity assay oxidative stress antibacterial properties biocompatibility assay human gingival fibroblast (HGFs) |
| url | https://www.mdpi.com/2571-6131/4/4/45 |
| work_keys_str_mv | AT konstantinakazeli synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties AT ioannistsamesidis synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties AT annatheocharidou synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties AT lamprinimalletzidou synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties AT jonathanrhoades synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties AT georgiakpouroutzidou synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties AT elenilikotrafiti synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties AT konstantinoschrissafis synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties AT theodoroslialiaris synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties AT lambrinipapadopoulou synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties AT eleanakontonasaki synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties AT evgenialymperaki synthesisandcharacterizationofnovelcalciumsilicatenanobioceramicswithmagnesiumeffectofheattreatmentonbiologicalphysicalandchemicalproperties |