Gens PSD‐95 and GSK‐3β expression improved by hair follicular stem cells‐conditioned medium enhances synaptic transmission and cognitive abilities in the rat model of vascular dementia
Abstract Introduction Vascular dementia (VaD) is a common type of dementia. The aim of this study was to investigate the cellular and molecular mechanism of conditioned medium (CM) in VaD. Material and methods The rats were divided into four groups of control (n = 9), sham‐operation (n = 10), VaD wi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Brain and Behavior |
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| Online Access: | https://doi.org/10.1002/brb3.3351 |
| _version_ | 1797263427128786944 |
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| author | Mojtaba Ghobadi Somayeh Akbari Mahnaz Bayat Seyed Mostafa Shid Moosavi Mohammad Saied Salehi Sareh Pandamooz Negar Azarpira Afsoon Afshari Etrat Hooshmandi Masoud Haghani |
| author_facet | Mojtaba Ghobadi Somayeh Akbari Mahnaz Bayat Seyed Mostafa Shid Moosavi Mohammad Saied Salehi Sareh Pandamooz Negar Azarpira Afsoon Afshari Etrat Hooshmandi Masoud Haghani |
| author_sort | Mojtaba Ghobadi |
| collection | DOAJ |
| description | Abstract Introduction Vascular dementia (VaD) is a common type of dementia. The aim of this study was to investigate the cellular and molecular mechanism of conditioned medium (CM) in VaD. Material and methods The rats were divided into four groups of control (n = 9), sham‐operation (n = 10), VaD with vehicle (n = 9), and VaD with CM (n = 12) that received CM on days 4, 14, and 24 after 2VO. Before sacrificing the rats, cognitive performance was assessed through the open‐field (OP), passive‐avoidance, and Morris‐water maze. The field‐potential recording was used to investigate basal synaptic transmission (BST) and long‐term potentiation (LTP). Subsequently, the hippocampus was dissected, and real‐time PCR was used to quantify the expression levels of β1‐catenin, insulin‐like growth factor‐1 (IGF‐1), transforming growth factor‐beta (TGF‐β), glycogen synthase kinase‐3β (GSK‐3β), postsynaptic density protein 95 (PSD‐95), and NR2B genes. Results The results indicated impaired performance in behavioral tests in 2VO rats, coupled with reductions in BST and LTP induction. The expression levels of β1‐catenin, IGF‐1, PSD‐95, and TGF‐β genes decreased, whereas NR2B and GSK‐3β expression increased. Treatment with CM restores the expression of PSD‐95 and GSK‐3β as well as fear‐memory, spatial learning, and grooming number without a positive effect on memory retrieval, time spent on the periphery and center of OP. The BST recovered upon administration of CM but, the LTP induction was still impaired. Conclusion The recovery of BST in VaD rats appears to be the most important outcome of this study which is caused by the improvement of gene expression and leads to the restoration of fear memory. |
| first_indexed | 2024-03-07T23:34:57Z |
| format | Article |
| id | doaj.art-dc5852e538e1462fb5ab2ecd12594305 |
| institution | Directory Open Access Journal |
| issn | 2162-3279 |
| language | English |
| last_indexed | 2024-04-25T00:12:50Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Brain and Behavior |
| spelling | doaj.art-dc5852e538e1462fb5ab2ecd125943052024-03-13T10:15:39ZengWileyBrain and Behavior2162-32792024-01-01141n/an/a10.1002/brb3.3351Gens PSD‐95 and GSK‐3β expression improved by hair follicular stem cells‐conditioned medium enhances synaptic transmission and cognitive abilities in the rat model of vascular dementiaMojtaba Ghobadi0Somayeh Akbari1Mahnaz Bayat2Seyed Mostafa Shid Moosavi3Mohammad Saied Salehi4Sareh Pandamooz5Negar Azarpira6Afsoon Afshari7Etrat Hooshmandi8Masoud Haghani9Department of Physiology Shiraz University of Medical Sciences Shiraz IranHistomorphometry and Stereology Research Centre Shiraz University of Medical Sciences Shiraz IranClinical Neurology Research Centre Shiraz University of Medical Sciences Shiraz IranDepartment of Physiology Shiraz University of Medical Sciences Shiraz IranClinical Neurology Research Centre Shiraz University of Medical Sciences Shiraz IranStem Cells Technology Research Center Shiraz University of Medical Sciences Shiraz IranShiraz Institute of Stem Cell and Regenerative Medicine Shiraz University of Medical Sciences Shiraz IranShiraz Nephro‐Urology Research Center Shiraz University of Medical Sciences Shiraz IranClinical Neurology Research Centre Shiraz University of Medical Sciences Shiraz IranDepartment of Physiology Shiraz University of Medical Sciences Shiraz IranAbstract Introduction Vascular dementia (VaD) is a common type of dementia. The aim of this study was to investigate the cellular and molecular mechanism of conditioned medium (CM) in VaD. Material and methods The rats were divided into four groups of control (n = 9), sham‐operation (n = 10), VaD with vehicle (n = 9), and VaD with CM (n = 12) that received CM on days 4, 14, and 24 after 2VO. Before sacrificing the rats, cognitive performance was assessed through the open‐field (OP), passive‐avoidance, and Morris‐water maze. The field‐potential recording was used to investigate basal synaptic transmission (BST) and long‐term potentiation (LTP). Subsequently, the hippocampus was dissected, and real‐time PCR was used to quantify the expression levels of β1‐catenin, insulin‐like growth factor‐1 (IGF‐1), transforming growth factor‐beta (TGF‐β), glycogen synthase kinase‐3β (GSK‐3β), postsynaptic density protein 95 (PSD‐95), and NR2B genes. Results The results indicated impaired performance in behavioral tests in 2VO rats, coupled with reductions in BST and LTP induction. The expression levels of β1‐catenin, IGF‐1, PSD‐95, and TGF‐β genes decreased, whereas NR2B and GSK‐3β expression increased. Treatment with CM restores the expression of PSD‐95 and GSK‐3β as well as fear‐memory, spatial learning, and grooming number without a positive effect on memory retrieval, time spent on the periphery and center of OP. The BST recovered upon administration of CM but, the LTP induction was still impaired. Conclusion The recovery of BST in VaD rats appears to be the most important outcome of this study which is caused by the improvement of gene expression and leads to the restoration of fear memory.https://doi.org/10.1002/brb3.3351conditioned mediumlong‐term potentiationmemorysynaptic transmissionvascular dementia |
| spellingShingle | Mojtaba Ghobadi Somayeh Akbari Mahnaz Bayat Seyed Mostafa Shid Moosavi Mohammad Saied Salehi Sareh Pandamooz Negar Azarpira Afsoon Afshari Etrat Hooshmandi Masoud Haghani Gens PSD‐95 and GSK‐3β expression improved by hair follicular stem cells‐conditioned medium enhances synaptic transmission and cognitive abilities in the rat model of vascular dementia Brain and Behavior conditioned medium long‐term potentiation memory synaptic transmission vascular dementia |
| title | Gens PSD‐95 and GSK‐3β expression improved by hair follicular stem cells‐conditioned medium enhances synaptic transmission and cognitive abilities in the rat model of vascular dementia |
| title_full | Gens PSD‐95 and GSK‐3β expression improved by hair follicular stem cells‐conditioned medium enhances synaptic transmission and cognitive abilities in the rat model of vascular dementia |
| title_fullStr | Gens PSD‐95 and GSK‐3β expression improved by hair follicular stem cells‐conditioned medium enhances synaptic transmission and cognitive abilities in the rat model of vascular dementia |
| title_full_unstemmed | Gens PSD‐95 and GSK‐3β expression improved by hair follicular stem cells‐conditioned medium enhances synaptic transmission and cognitive abilities in the rat model of vascular dementia |
| title_short | Gens PSD‐95 and GSK‐3β expression improved by hair follicular stem cells‐conditioned medium enhances synaptic transmission and cognitive abilities in the rat model of vascular dementia |
| title_sort | gens psd 95 and gsk 3β expression improved by hair follicular stem cells conditioned medium enhances synaptic transmission and cognitive abilities in the rat model of vascular dementia |
| topic | conditioned medium long‐term potentiation memory synaptic transmission vascular dementia |
| url | https://doi.org/10.1002/brb3.3351 |
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