Lack of association between polymorphisms of <it>MASP2 </it>and susceptibility to SARS coronavirus infection
<p>Abstract</p> <p>Background</p> <p>The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and <it>mannan-binding lectin </it>(<it>...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-05-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/9/51 |
| _version_ | 1811315493775081472 |
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| author | Yang Ruifu Ma Qingjun Liu Chuanxuan Li Ping Shi Yuling Yan Jiangwei Wang Yan Wang Xiaoyi zhu Lina Yang Xiao Cao Cheng |
| author_facet | Yang Ruifu Ma Qingjun Liu Chuanxuan Li Ping Shi Yuling Yan Jiangwei Wang Yan Wang Xiaoyi zhu Lina Yang Xiao Cao Cheng |
| author_sort | Yang Ruifu |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and <it>mannan-binding lectin </it>(<it>MBL</it>) in China. As the downstream protein of <it>MBL</it>, variants of the <it>MBL</it>-associated serine protease-2 (<it>MASP2</it>) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.</p> <p>Methods</p> <p>Thirty individuals with SARS were chosen for analysis of <it>MASP2 </it>polymorphisms by means of PCR direct sequencing. Tag single nucleotide polymorphisms (tagSNPs) were chosen using pairwise tagging algorithms. The frequencies of four tag SNPs (rs12711521, rs2261695, rs2273346 and rs7548659) were ascertained in 376 SARS patients and 523 control subjects, using the Beckman SNPstream Ultra High Throughput genotyping platform.</p> <p>Results</p> <p>There is no significant association between alleles or genotypes of the <it>MASP2 </it>tagSNP and susceptibility to SARS-CoV in both Beijing and Guangzhou populations. Diplotype (rs2273346 and rs12711521)were analyzed for association with susceptibility to SARS, no statistically significant evidence of association was observed. The Beijing and Guangzhou sample groups were homogeneous regarding demographic and genetic parameters, a joined analysis also showed no statistically significant evidence of association.</p> <p>Conclusion</p> <p>Our data do not suggest a role for <it>MASP2 </it>polymorphisms in SARS susceptibility in northern and southern China.</p> |
| first_indexed | 2024-04-13T11:31:14Z |
| format | Article |
| id | doaj.art-dc59a421bacb4e2299ca4fe71785e091 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-13T11:31:14Z |
| publishDate | 2009-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-dc59a421bacb4e2299ca4fe71785e0912022-12-22T02:48:33ZengBMCBMC Infectious Diseases1471-23342009-05-01915110.1186/1471-2334-9-51Lack of association between polymorphisms of <it>MASP2 </it>and susceptibility to SARS coronavirus infectionYang RuifuMa QingjunLiu ChuanxuanLi PingShi YulingYan JiangweiWang YanWang Xiaoyizhu LinaYang XiaoCao Cheng<p>Abstract</p> <p>Background</p> <p>The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and <it>mannan-binding lectin </it>(<it>MBL</it>) in China. As the downstream protein of <it>MBL</it>, variants of the <it>MBL</it>-associated serine protease-2 (<it>MASP2</it>) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.</p> <p>Methods</p> <p>Thirty individuals with SARS were chosen for analysis of <it>MASP2 </it>polymorphisms by means of PCR direct sequencing. Tag single nucleotide polymorphisms (tagSNPs) were chosen using pairwise tagging algorithms. The frequencies of four tag SNPs (rs12711521, rs2261695, rs2273346 and rs7548659) were ascertained in 376 SARS patients and 523 control subjects, using the Beckman SNPstream Ultra High Throughput genotyping platform.</p> <p>Results</p> <p>There is no significant association between alleles or genotypes of the <it>MASP2 </it>tagSNP and susceptibility to SARS-CoV in both Beijing and Guangzhou populations. Diplotype (rs2273346 and rs12711521)were analyzed for association with susceptibility to SARS, no statistically significant evidence of association was observed. The Beijing and Guangzhou sample groups were homogeneous regarding demographic and genetic parameters, a joined analysis also showed no statistically significant evidence of association.</p> <p>Conclusion</p> <p>Our data do not suggest a role for <it>MASP2 </it>polymorphisms in SARS susceptibility in northern and southern China.</p>http://www.biomedcentral.com/1471-2334/9/51 |
| spellingShingle | Yang Ruifu Ma Qingjun Liu Chuanxuan Li Ping Shi Yuling Yan Jiangwei Wang Yan Wang Xiaoyi zhu Lina Yang Xiao Cao Cheng Lack of association between polymorphisms of <it>MASP2 </it>and susceptibility to SARS coronavirus infection BMC Infectious Diseases |
| title | Lack of association between polymorphisms of <it>MASP2 </it>and susceptibility to SARS coronavirus infection |
| title_full | Lack of association between polymorphisms of <it>MASP2 </it>and susceptibility to SARS coronavirus infection |
| title_fullStr | Lack of association between polymorphisms of <it>MASP2 </it>and susceptibility to SARS coronavirus infection |
| title_full_unstemmed | Lack of association between polymorphisms of <it>MASP2 </it>and susceptibility to SARS coronavirus infection |
| title_short | Lack of association between polymorphisms of <it>MASP2 </it>and susceptibility to SARS coronavirus infection |
| title_sort | lack of association between polymorphisms of it masp2 it and susceptibility to sars coronavirus infection |
| url | http://www.biomedcentral.com/1471-2334/9/51 |
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