Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown Daucus L. Species in Turkey
Objective:This present study was conducted to determine the interspecific chemical variability and evaluate the biological effects of the essential oils of Daucus L. species growing naturally in Turkey. The species were D. carota, D. broteri, D. guttatus, D. littoralis, D. involucratus and D. conchi...
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| Format: | Article |
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Galenos Publishing House
2022-12-01
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| Series: | Bezmiâlem Science |
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http://bezmialemscience.org/archives/archive-detail/article-preview/anticholinesterase-and-anti-inflammatory-activitie/51208
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| author | Betül BÜYÜKKILIÇ ALTINBAŞAK Gülay ECEVİT GENÇ Belma ZENGİN KURT Betül DEMİRCİ |
| author_facet | Betül BÜYÜKKILIÇ ALTINBAŞAK Gülay ECEVİT GENÇ Belma ZENGİN KURT Betül DEMİRCİ |
| author_sort | Betül BÜYÜKKILIÇ ALTINBAŞAK |
| collection | DOAJ |
| description | Objective:This present study was conducted to determine the interspecific chemical variability and evaluate the biological effects of the essential oils of Daucus L. species growing naturally in Turkey. The species were D. carota, D. broteri, D. guttatus, D. littoralis, D. involucratus and D. conchitae (endemic).Methods:The essential oils were obtained from fruit samples by distillation method and were analyzed both by GC-FID and GC-MS. The anti-inflammatory and anticholinesterase effects of the essential oils were investigated. The anti-inflammatory effect was evaluated by in vitro LOX enzyme inhibition activity. The anticholinesterase effect was tested on AChE and BChE enzymes.Results:The components, ratios, and yields of D. carota essential oils differed depending on the locations where the samples were collected. The main components were detected as carotol (1-74.6%), β-bisabolen (0.9-62.4%), 11αH-himachal-4-en-1β-ol (0.3-49.4%), trans-methylisoeugenol (1-45.7%). The main volatile compounds were found in D. broteri, D. guttatus, D. involucratus, D. littoralis, D. conchitae as β-sinensal (30.4%), methyleugenol (30.5%), methyleugenol (40.9%), α-humulene (29.4%), methyleugenol (29.6%) respectively. The essential oils didn’t exhibit anti-inflammatory activity. Two essential oil samples of D. carota showed high anticholinesterase effects compared to the standard. The AChE IC50 was calculated as 6.04±0.30 μg/mL, 2.15±0.10 μg/mL (Galantamine IC50 1.13±0.02 μg/mL) and BChE IC50 11.32±0.20 μg/mL, 31.03±0.02 μg/mL (Galantamine IC50 12.15±0.36 μg/mL). These essential oils contained high levels of 11αH-himachal-4-en-1β-ol (25.04%, 49.42%).Conclusion:Because of their anticholinesterase potential, some D. carote essential oils can be evaluated in the preparation of pharmaceutical or nutraceutical products as a complementary therapy for Alzheimer’s disease by standardizing their components. |
| first_indexed | 2024-04-10T11:23:53Z |
| format | Article |
| id | doaj.art-dc5a2adf85104c3da3567849c2f4f5e5 |
| institution | Directory Open Access Journal |
| issn | 2148-2373 |
| language | English |
| last_indexed | 2024-04-10T11:23:53Z |
| publishDate | 2022-12-01 |
| publisher | Galenos Publishing House |
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| series | Bezmiâlem Science |
| spelling | doaj.art-dc5a2adf85104c3da3567849c2f4f5e52023-02-15T16:18:32ZengGalenos Publishing HouseBezmiâlem Science2148-23732022-12-0110672273410.14235/bas.galenos.2022.3792913049054Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown Daucus L. Species in TurkeyBetül BÜYÜKKILIÇ ALTINBAŞAK0Gülay ECEVİT GENÇ1Belma ZENGİN KURT2Betül DEMİRCİ3 Kocaeli Health and Technology University Faculty of Pharmacy, Department of Pharmacognosy, Kocaeli, Turkey İstanbul University Faculty of Pharmacy, Department of Pharmaceutical Botany, İstanbul, Turkey Bezmialem Vakıf University Faculty of Pharmacy, Department of Pharmaceutical Chemistry, İstanbul, Turkey Anadolu University Faculty of Pharmacy, Department of Pharmacognosy, Eskişehir, Turkey Objective:This present study was conducted to determine the interspecific chemical variability and evaluate the biological effects of the essential oils of Daucus L. species growing naturally in Turkey. The species were D. carota, D. broteri, D. guttatus, D. littoralis, D. involucratus and D. conchitae (endemic).Methods:The essential oils were obtained from fruit samples by distillation method and were analyzed both by GC-FID and GC-MS. The anti-inflammatory and anticholinesterase effects of the essential oils were investigated. The anti-inflammatory effect was evaluated by in vitro LOX enzyme inhibition activity. The anticholinesterase effect was tested on AChE and BChE enzymes.Results:The components, ratios, and yields of D. carota essential oils differed depending on the locations where the samples were collected. The main components were detected as carotol (1-74.6%), β-bisabolen (0.9-62.4%), 11αH-himachal-4-en-1β-ol (0.3-49.4%), trans-methylisoeugenol (1-45.7%). The main volatile compounds were found in D. broteri, D. guttatus, D. involucratus, D. littoralis, D. conchitae as β-sinensal (30.4%), methyleugenol (30.5%), methyleugenol (40.9%), α-humulene (29.4%), methyleugenol (29.6%) respectively. The essential oils didn’t exhibit anti-inflammatory activity. Two essential oil samples of D. carota showed high anticholinesterase effects compared to the standard. The AChE IC50 was calculated as 6.04±0.30 μg/mL, 2.15±0.10 μg/mL (Galantamine IC50 1.13±0.02 μg/mL) and BChE IC50 11.32±0.20 μg/mL, 31.03±0.02 μg/mL (Galantamine IC50 12.15±0.36 μg/mL). These essential oils contained high levels of 11αH-himachal-4-en-1β-ol (25.04%, 49.42%).Conclusion:Because of their anticholinesterase potential, some D. carote essential oils can be evaluated in the preparation of pharmaceutical or nutraceutical products as a complementary therapy for Alzheimer’s disease by standardizing their components. http://bezmialemscience.org/archives/archive-detail/article-preview/anticholinesterase-and-anti-inflammatory-activitie/51208 daucus spp.essential oilanti-inflammatoryanticholinesterase |
| spellingShingle | Betül BÜYÜKKILIÇ ALTINBAŞAK Gülay ECEVİT GENÇ Belma ZENGİN KURT Betül DEMİRCİ Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown Daucus L. Species in Turkey Bezmiâlem Science daucus spp. essential oil anti-inflammatory anticholinesterase |
| title | Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown Daucus L. Species in Turkey |
| title_full | Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown Daucus L. Species in Turkey |
| title_fullStr | Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown Daucus L. Species in Turkey |
| title_full_unstemmed | Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown Daucus L. Species in Turkey |
| title_short | Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown Daucus L. Species in Turkey |
| title_sort | anticholinesterase and anti inflammatory activities of essential oils of naturally grown daucus l species in turkey |
| topic | daucus spp. essential oil anti-inflammatory anticholinesterase |
| url |
http://bezmialemscience.org/archives/archive-detail/article-preview/anticholinesterase-and-anti-inflammatory-activitie/51208
|
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