Changes in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthma
Abstract Background To investigate the changes of polyunsaturated fatty acids (PUFAs) and their downstream eicosanoids in patients with asthma, the levels of erythrocyte membrane lipids and plasma lipid metabolites were examined. Methods Erythrocyte membrane lipids were extracted and esterificated,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-018-0853-y |
| _version_ | 1818573194015014912 |
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| author | Jing Zhou Lifang Chen Zhenjie Liu Ling Sang Yimin Li Dongjuan Yuan |
| author_facet | Jing Zhou Lifang Chen Zhenjie Liu Ling Sang Yimin Li Dongjuan Yuan |
| author_sort | Jing Zhou |
| collection | DOAJ |
| description | Abstract Background To investigate the changes of polyunsaturated fatty acids (PUFAs) and their downstream eicosanoids in patients with asthma, the levels of erythrocyte membrane lipids and plasma lipid metabolites were examined. Methods Erythrocyte membrane lipids were extracted and esterificated, and then fatty acid compositions were determined by gas chromatography. The concentrations of six eicosanoids of PGE2, TXA2, LTB4, PGE1, 6-k-PGF1α and PGF2α in plasma were measured by ELISA. Results The results showed that the contents of erythrocyte membrane fatty acids in patients with asthma were mainly composed of C16:0, C18:0, C18:1, C18:2(n-6), and C20:4(n-6). The ratio n-6/n-3 PUFAs in patients and health persons were (4.42 ± 1.33):1 and (3.21 ± 0.79):1 (p < 0.01), showing statistically significant differences. ELISA results showed that the levels of plasma PGE2, TXB2, and PGE1 in patients were higher than health persons; and the levels of eicosanoids of PGF2α and 6-k-PGF1α were significantly lower in patient group than healthy group (p < 0.05), but LTB4 was no obvious difference (p = 0.09). Increased ratio of n-6/n-3 PUFAs is consistent to the increased levels of pro-inflammatory PGE2 and TXB2 and anti-inflammatory PGE1 originated from C20:4(n-6) and C18:2(n-6), indicating that increased ratio of n-6/n-3 PUFAs and eicosanoids from n-6 PUFAs might promote the progress of airway inflammation of asthma. Conclusion Changes of erythrocyte fatty acids, n-6/n-3 PUFAs ratio and the levels of plasma PGE2, TXB2, and PGE1 in patients with asthma were relevant to airway inflammation in some extent. Therefore, it could be proposed that increase of n-3/n-6 PUFAs ratio by diet supplementation of n-3 PUFAs might effectively improve airway inflammation in asthma. |
| first_indexed | 2024-12-15T00:07:55Z |
| format | Article |
| id | doaj.art-dc5a57730abb4fdeb9b236a1956fe880 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-15T00:07:55Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-dc5a57730abb4fdeb9b236a1956fe8802022-12-21T22:42:41ZengBMCLipids in Health and Disease1476-511X2018-09-011711610.1186/s12944-018-0853-yChanges in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthmaJing Zhou0Lifang Chen1Zhenjie Liu2Ling Sang3Yimin Li4Dongjuan Yuan5Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory HealthDepartment of Respiratory, The affiliated hospital of Guangdong medical UniversityThe Second Affiliated Hospital of Guangdong University of Chinese MedicineDepartment of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory HealthDepartment of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory HealthCollege of Veterinary Medicine, South China Agricultural UniversityAbstract Background To investigate the changes of polyunsaturated fatty acids (PUFAs) and their downstream eicosanoids in patients with asthma, the levels of erythrocyte membrane lipids and plasma lipid metabolites were examined. Methods Erythrocyte membrane lipids were extracted and esterificated, and then fatty acid compositions were determined by gas chromatography. The concentrations of six eicosanoids of PGE2, TXA2, LTB4, PGE1, 6-k-PGF1α and PGF2α in plasma were measured by ELISA. Results The results showed that the contents of erythrocyte membrane fatty acids in patients with asthma were mainly composed of C16:0, C18:0, C18:1, C18:2(n-6), and C20:4(n-6). The ratio n-6/n-3 PUFAs in patients and health persons were (4.42 ± 1.33):1 and (3.21 ± 0.79):1 (p < 0.01), showing statistically significant differences. ELISA results showed that the levels of plasma PGE2, TXB2, and PGE1 in patients were higher than health persons; and the levels of eicosanoids of PGF2α and 6-k-PGF1α were significantly lower in patient group than healthy group (p < 0.05), but LTB4 was no obvious difference (p = 0.09). Increased ratio of n-6/n-3 PUFAs is consistent to the increased levels of pro-inflammatory PGE2 and TXB2 and anti-inflammatory PGE1 originated from C20:4(n-6) and C18:2(n-6), indicating that increased ratio of n-6/n-3 PUFAs and eicosanoids from n-6 PUFAs might promote the progress of airway inflammation of asthma. Conclusion Changes of erythrocyte fatty acids, n-6/n-3 PUFAs ratio and the levels of plasma PGE2, TXB2, and PGE1 in patients with asthma were relevant to airway inflammation in some extent. Therefore, it could be proposed that increase of n-3/n-6 PUFAs ratio by diet supplementation of n-3 PUFAs might effectively improve airway inflammation in asthma.http://link.springer.com/article/10.1186/s12944-018-0853-yAsthmaPolyunsaturated fatty acidsEicosanoidsInflammation |
| spellingShingle | Jing Zhou Lifang Chen Zhenjie Liu Ling Sang Yimin Li Dongjuan Yuan Changes in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthma Lipids in Health and Disease Asthma Polyunsaturated fatty acids Eicosanoids Inflammation |
| title | Changes in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthma |
| title_full | Changes in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthma |
| title_fullStr | Changes in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthma |
| title_full_unstemmed | Changes in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthma |
| title_short | Changes in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthma |
| title_sort | changes in erythrocyte polyunsaturated fatty acids and plasma eicosanoids level in patients with asthma |
| topic | Asthma Polyunsaturated fatty acids Eicosanoids Inflammation |
| url | http://link.springer.com/article/10.1186/s12944-018-0853-y |
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