Role of Bclaf1 in Promoting Adrenocortical Carcinoma Proliferation: A Study Combining the Use of Bioinformatics and Molecular Events
Hui Zhou,1,* Mengchen Zou,2,* Xinyi Ding,1 Fei Zou1 1Department of Occupational Health and Occupational Medicine, School of Public Health, Southern Medical University, Guangzhou, People’s Republic of China; 2Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medic...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-08-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/role-of-bclaf1-in-promoting-adrenocortical-carcinoma-proliferation-a-s-peer-reviewed-fulltext-article-CMAR |
| _version_ | 1819062974058332160 |
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| author | Zhou H Zou M Ding X Zou F |
| author_facet | Zhou H Zou M Ding X Zou F |
| author_sort | Zhou H |
| collection | DOAJ |
| description | Hui Zhou,1,* Mengchen Zou,2,* Xinyi Ding,1 Fei Zou1 1Department of Occupational Health and Occupational Medicine, School of Public Health, Southern Medical University, Guangzhou, People’s Republic of China; 2Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fei ZouDepartment of Occupational Health and Occupational Medicine, School of Public Health, Southern Medical University, Guangzhou, 510515, People’s Republic of ChinaEmail zfei@smu.edu.cnPurpose: Adrenocortical carcinoma (ACC) is a rare malignancy with poor prognosis, and researchers are interested in further studying its diagnosis and treatment. Our study aims to identify new potential therapeutic targets in ACC.Patients and Methods: The core genes CDK1 and CCNB1 were previously screened using ACC data from The Cancer Genome Atlas (TCGA) as the most relevant to Bclaf1 and tumour prognosis. We used siRNA- or shRNA-based models to explore the role of Bcl-2-associated transcription factor 1 (Bclaf1) in SW-13 cell lines. Western blotting and qPCR were used to determine the effects of Bclaf1 on CDK1 and Cyclin B1.Results: Based on biological information analysis, we found that Bcl-2-associated transcription factor 1 (Bclaf1) affected the progression of ACC and was associated with the cell cycle. Downregulated Bclaf1 expression inhibited the proliferation of SW-13 cells and affected the cell cycle process of SW-13 cells. BCLAF1 was correlated with CDK1 and CCNB1 and can regulate their mRNA and protein levels.Conclusion: Bclaf1 might promote the development of ACC by regulating CDK1 and Cyclin B1 to drive mitosis.Keywords: prognostic and predictive value, therapeutic targets, Bcl-2-associated transcription factor 1 |
| first_indexed | 2024-12-21T15:07:18Z |
| format | Article |
| id | doaj.art-dc5ca1d449984d7ba08d9be968d75a93 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-21T15:07:18Z |
| publishDate | 2021-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-dc5ca1d449984d7ba08d9be968d75a932022-12-21T18:59:24ZengDove Medical PressCancer Management and Research1179-13222021-08-01Volume 136785679568386Role of Bclaf1 in Promoting Adrenocortical Carcinoma Proliferation: A Study Combining the Use of Bioinformatics and Molecular EventsZhou HZou MDing XZou FHui Zhou,1,* Mengchen Zou,2,* Xinyi Ding,1 Fei Zou1 1Department of Occupational Health and Occupational Medicine, School of Public Health, Southern Medical University, Guangzhou, People’s Republic of China; 2Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fei ZouDepartment of Occupational Health and Occupational Medicine, School of Public Health, Southern Medical University, Guangzhou, 510515, People’s Republic of ChinaEmail zfei@smu.edu.cnPurpose: Adrenocortical carcinoma (ACC) is a rare malignancy with poor prognosis, and researchers are interested in further studying its diagnosis and treatment. Our study aims to identify new potential therapeutic targets in ACC.Patients and Methods: The core genes CDK1 and CCNB1 were previously screened using ACC data from The Cancer Genome Atlas (TCGA) as the most relevant to Bclaf1 and tumour prognosis. We used siRNA- or shRNA-based models to explore the role of Bcl-2-associated transcription factor 1 (Bclaf1) in SW-13 cell lines. Western blotting and qPCR were used to determine the effects of Bclaf1 on CDK1 and Cyclin B1.Results: Based on biological information analysis, we found that Bcl-2-associated transcription factor 1 (Bclaf1) affected the progression of ACC and was associated with the cell cycle. Downregulated Bclaf1 expression inhibited the proliferation of SW-13 cells and affected the cell cycle process of SW-13 cells. BCLAF1 was correlated with CDK1 and CCNB1 and can regulate their mRNA and protein levels.Conclusion: Bclaf1 might promote the development of ACC by regulating CDK1 and Cyclin B1 to drive mitosis.Keywords: prognostic and predictive value, therapeutic targets, Bcl-2-associated transcription factor 1https://www.dovepress.com/role-of-bclaf1-in-promoting-adrenocortical-carcinoma-proliferation-a-s-peer-reviewed-fulltext-article-CMARprognostic and predictive valuetherapeutic targetsbcl-2-associated transcription factor 1 |
| spellingShingle | Zhou H Zou M Ding X Zou F Role of Bclaf1 in Promoting Adrenocortical Carcinoma Proliferation: A Study Combining the Use of Bioinformatics and Molecular Events Cancer Management and Research prognostic and predictive value therapeutic targets bcl-2-associated transcription factor 1 |
| title | Role of Bclaf1 in Promoting Adrenocortical Carcinoma Proliferation: A Study Combining the Use of Bioinformatics and Molecular Events |
| title_full | Role of Bclaf1 in Promoting Adrenocortical Carcinoma Proliferation: A Study Combining the Use of Bioinformatics and Molecular Events |
| title_fullStr | Role of Bclaf1 in Promoting Adrenocortical Carcinoma Proliferation: A Study Combining the Use of Bioinformatics and Molecular Events |
| title_full_unstemmed | Role of Bclaf1 in Promoting Adrenocortical Carcinoma Proliferation: A Study Combining the Use of Bioinformatics and Molecular Events |
| title_short | Role of Bclaf1 in Promoting Adrenocortical Carcinoma Proliferation: A Study Combining the Use of Bioinformatics and Molecular Events |
| title_sort | role of bclaf1 in promoting adrenocortical carcinoma proliferation a study combining the use of bioinformatics and molecular events |
| topic | prognostic and predictive value therapeutic targets bcl-2-associated transcription factor 1 |
| url | https://www.dovepress.com/role-of-bclaf1-in-promoting-adrenocortical-carcinoma-proliferation-a-s-peer-reviewed-fulltext-article-CMAR |
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