Development of a Novel BAFF Responsive Cell Line Suitable for Detecting Bioactive BAFF and Neutralizing Antibodies against BAFF-Pathway Inhibiting Therapeutics
BAFF has a critical role in B-cell survival, maturation and function, which makes its pathway a prime therapeutic target for various autoimmune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis and Sjögren’s syndrome. A cell-based assay that measures the functional activity...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-02-01
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| Series: | Cells |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2073-4409/3/1/79 |
| _version_ | 1797707558153093120 |
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| author | Jenny Hu Yanbin Yu Hong Han Francesca Civoli Yao Zhuang John Thomas Steve Swanson Shuqian Jing Shalini Gupta |
| author_facet | Jenny Hu Yanbin Yu Hong Han Francesca Civoli Yao Zhuang John Thomas Steve Swanson Shuqian Jing Shalini Gupta |
| author_sort | Jenny Hu |
| collection | DOAJ |
| description | BAFF has a critical role in B-cell survival, maturation and function, which makes its pathway a prime therapeutic target for various autoimmune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis and Sjögren’s syndrome. A cell-based assay that measures the functional activity of BAFF is required for many high throughput purposes, such as lead target screening and BAFF quantification. We report here the development of a sensitive BAFF responsive cell line via stable transfection of the BAFFR-TNFR1 hybrid receptor into monkey kidney epithelial COS-1 cells. The cellular response to BAFF can be detected by measuring the secretion of IL-8. This BAFF bioassay is not only reproducible and sensitive, but also responsive to a wide concentration range of BAFF stimulation in sera from various species. This cell line is useful in the development of sensitive bioassays to measure the levels of bioactive BAFF, inhibition of BAFF and neutralizing antibodies against any BAFF pathway-mediated therapeutic proteins. |
| first_indexed | 2024-03-12T06:09:05Z |
| format | Article |
| id | doaj.art-dc5e74bbeb364f6dbd8629aadc73adbc |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:09:05Z |
| publishDate | 2014-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-dc5e74bbeb364f6dbd8629aadc73adbc2023-09-03T03:22:37ZengMDPI AGCells2073-44092014-02-0131799110.3390/cells3010079cells3010079Development of a Novel BAFF Responsive Cell Line Suitable for Detecting Bioactive BAFF and Neutralizing Antibodies against BAFF-Pathway Inhibiting TherapeuticsJenny Hu0Yanbin Yu1Hong Han2Francesca Civoli3Yao Zhuang4John Thomas5Steve Swanson6Shuqian Jing7Shalini Gupta8Amgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320, USAAmgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320, USAAmgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320, USAAmgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320, USAAmgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320, USAAmgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320, USAAmgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320, USAGMAX Biopharm., 288 Qiuyi Road, Binjiang District, Hangzhou 310052, Zhejiang, ChinaAmgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320, USABAFF has a critical role in B-cell survival, maturation and function, which makes its pathway a prime therapeutic target for various autoimmune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis and Sjögren’s syndrome. A cell-based assay that measures the functional activity of BAFF is required for many high throughput purposes, such as lead target screening and BAFF quantification. We report here the development of a sensitive BAFF responsive cell line via stable transfection of the BAFFR-TNFR1 hybrid receptor into monkey kidney epithelial COS-1 cells. The cellular response to BAFF can be detected by measuring the secretion of IL-8. This BAFF bioassay is not only reproducible and sensitive, but also responsive to a wide concentration range of BAFF stimulation in sera from various species. This cell line is useful in the development of sensitive bioassays to measure the levels of bioactive BAFF, inhibition of BAFF and neutralizing antibodies against any BAFF pathway-mediated therapeutic proteins.http://www.mdpi.com/2073-4409/3/1/79hybrid receptorneutralizing antibody detectionBAFF responseserum matrixtransfectionCOS-1 cell line |
| spellingShingle | Jenny Hu Yanbin Yu Hong Han Francesca Civoli Yao Zhuang John Thomas Steve Swanson Shuqian Jing Shalini Gupta Development of a Novel BAFF Responsive Cell Line Suitable for Detecting Bioactive BAFF and Neutralizing Antibodies against BAFF-Pathway Inhibiting Therapeutics Cells hybrid receptor neutralizing antibody detection BAFF response serum matrix transfection COS-1 cell line |
| title | Development of a Novel BAFF Responsive Cell Line Suitable for Detecting Bioactive BAFF and Neutralizing Antibodies against BAFF-Pathway Inhibiting Therapeutics |
| title_full | Development of a Novel BAFF Responsive Cell Line Suitable for Detecting Bioactive BAFF and Neutralizing Antibodies against BAFF-Pathway Inhibiting Therapeutics |
| title_fullStr | Development of a Novel BAFF Responsive Cell Line Suitable for Detecting Bioactive BAFF and Neutralizing Antibodies against BAFF-Pathway Inhibiting Therapeutics |
| title_full_unstemmed | Development of a Novel BAFF Responsive Cell Line Suitable for Detecting Bioactive BAFF and Neutralizing Antibodies against BAFF-Pathway Inhibiting Therapeutics |
| title_short | Development of a Novel BAFF Responsive Cell Line Suitable for Detecting Bioactive BAFF and Neutralizing Antibodies against BAFF-Pathway Inhibiting Therapeutics |
| title_sort | development of a novel baff responsive cell line suitable for detecting bioactive baff and neutralizing antibodies against baff pathway inhibiting therapeutics |
| topic | hybrid receptor neutralizing antibody detection BAFF response serum matrix transfection COS-1 cell line |
| url | http://www.mdpi.com/2073-4409/3/1/79 |
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