The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected?
To our knowledge, there are no reports that demonstrate the use of host molecular markers for the purpose of detecting generic plant virus infection. Two approaches involving molecular indicators of virus infection in the model plant <i>Arabidopsis thaliana</i> were examined: the accumul...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/2/188 |
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| author | Gardette R. Valmonte-Cortes Sonia T. Lilly Michael N. Pearson Colleen M. Higgins Robin M. MacDiarmid |
| author_facet | Gardette R. Valmonte-Cortes Sonia T. Lilly Michael N. Pearson Colleen M. Higgins Robin M. MacDiarmid |
| author_sort | Gardette R. Valmonte-Cortes |
| collection | DOAJ |
| description | To our knowledge, there are no reports that demonstrate the use of host molecular markers for the purpose of detecting generic plant virus infection. Two approaches involving molecular indicators of virus infection in the model plant <i>Arabidopsis thaliana</i> were examined: the accumulation of small RNAs (sRNAs) using a microfluidics-based method (Bioanalyzer); and the transcript accumulation of virus-response related host plant genes, suppressor of gene silencing 3 (<i>AtSGS3</i>) and calcium-dependent protein kinase 3 (<i>AtCPK3</i>) by reverse transcriptase-quantitative PCR (RT-qPCR). The microfluidics approach using sRNA chips has previously demonstrated good linearity and good reproducibility, both within and between chips. Good limits of detection have been demonstrated from two-fold 10-point serial dilution regression to 0.1 ng of RNA. The ratio of small RNA (sRNA) to ribosomal RNA (rRNA), as a proportion of averaged mock-inoculation, correlated with known virus infection to a high degree of certainty. <i>AtSGS3</i> transcript decreased between 14- and 28-days post inoculation (dpi) for all viruses investigated, while <i>AtCPK3</i> transcript increased between 14 and 28 dpi for all viruses. A combination of these two molecular approaches may be useful for assessment of virus-infection of samples without the need for diagnosis of specific virus infection. |
| first_indexed | 2024-03-10T00:41:40Z |
| format | Article |
| id | doaj.art-dc5f28876fce4220aacba01703c940e7 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T00:41:40Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-dc5f28876fce4220aacba01703c940e72023-11-23T15:07:18ZengMDPI AGPlants2223-77472022-01-0111218810.3390/plants11020188The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected?Gardette R. Valmonte-Cortes0Sonia T. Lilly1Michael N. Pearson2Colleen M. Higgins3Robin M. MacDiarmid4School of Science, AUT City Campus, Auckland University of Technology, Auckland 1142, New ZealandThe New Zealand Institute for Plant & Food Research Limited, 120 Mt Albert Road, Auckland 1025, New ZealandSchool of Biological Sciences, The University of Auckland, Thomas Building, 3a Symonds Street, Auckland 1010, New ZealandSchool of Science, AUT City Campus, Auckland University of Technology, Auckland 1142, New ZealandThe New Zealand Institute for Plant & Food Research Limited, 120 Mt Albert Road, Auckland 1025, New ZealandTo our knowledge, there are no reports that demonstrate the use of host molecular markers for the purpose of detecting generic plant virus infection. Two approaches involving molecular indicators of virus infection in the model plant <i>Arabidopsis thaliana</i> were examined: the accumulation of small RNAs (sRNAs) using a microfluidics-based method (Bioanalyzer); and the transcript accumulation of virus-response related host plant genes, suppressor of gene silencing 3 (<i>AtSGS3</i>) and calcium-dependent protein kinase 3 (<i>AtCPK3</i>) by reverse transcriptase-quantitative PCR (RT-qPCR). The microfluidics approach using sRNA chips has previously demonstrated good linearity and good reproducibility, both within and between chips. Good limits of detection have been demonstrated from two-fold 10-point serial dilution regression to 0.1 ng of RNA. The ratio of small RNA (sRNA) to ribosomal RNA (rRNA), as a proportion of averaged mock-inoculation, correlated with known virus infection to a high degree of certainty. <i>AtSGS3</i> transcript decreased between 14- and 28-days post inoculation (dpi) for all viruses investigated, while <i>AtCPK3</i> transcript increased between 14 and 28 dpi for all viruses. A combination of these two molecular approaches may be useful for assessment of virus-infection of samples without the need for diagnosis of specific virus infection.https://www.mdpi.com/2223-7747/11/2/188plant virusdetectionsRNAmolecular markersCPK<i>SGS3</i> |
| spellingShingle | Gardette R. Valmonte-Cortes Sonia T. Lilly Michael N. Pearson Colleen M. Higgins Robin M. MacDiarmid The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected? Plants plant virus detection sRNA molecular markers CPK <i>SGS3</i> |
| title | The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected? |
| title_full | The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected? |
| title_fullStr | The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected? |
| title_full_unstemmed | The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected? |
| title_short | The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected? |
| title_sort | potential of molecular indicators of plant virus infection are plants able to tell us they are infected |
| topic | plant virus detection sRNA molecular markers CPK <i>SGS3</i> |
| url | https://www.mdpi.com/2223-7747/11/2/188 |
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