Effects of Temperature on Lifespan of <i>Drosophila melanogaster</i> from Different Genetic Backgrounds: Links between Metabolic Rate and Longevity
Despite many studies of the aging process, questions about key factors ensuring longevity have not yet found clear answers. Temperature seems to be one of the most important factors regulating lifespan. However, the genetic background may also play a key role in determining longevity. The aim of thi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/11/8/470 |
| _version_ | 1827712404829503488 |
|---|---|
| author | Mateusz Mołoń Jan Dampc Monika Kula-Maximenko Jacek Zebrowski Agnieszka Mołoń Ralph Dobler Roma Durak Andrzej Skoczowski |
| author_facet | Mateusz Mołoń Jan Dampc Monika Kula-Maximenko Jacek Zebrowski Agnieszka Mołoń Ralph Dobler Roma Durak Andrzej Skoczowski |
| author_sort | Mateusz Mołoń |
| collection | DOAJ |
| description | Despite many studies of the aging process, questions about key factors ensuring longevity have not yet found clear answers. Temperature seems to be one of the most important factors regulating lifespan. However, the genetic background may also play a key role in determining longevity. The aim of this study was to investigate the relationship between the temperature, genetic background (fruit fly origin), and metabolic rate on lifespan. Experiments were performed with the use of the wild type <i>Drosophila melanogaster</i> fruit flies originating from Australia, Canada, and Benin and the reference OregonR strain. The metabolic rate of <i>D. melanogaster</i> was measured at 20 °C, 25 °C, and 28 °C in an isothermal calorimeter. We found a strong negative relationship between the total heat flow and longevity. A high metabolic rate leads to increased aging in males and females in all strains. Furthermore, our results showed that temperature has a significant effect on fecundity and body weight. We also showed the usefulness of the isothermal calorimetry method to study the effect of environmental stress conditions on the metabolic activity of insects. This may be particularly important for the forecasting of impact of global warming on metabolic activity and lifespan of various insects. |
| first_indexed | 2024-03-10T18:13:29Z |
| format | Article |
| id | doaj.art-dc5ff7a8069e4fb9a4feee34a70d82bc |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T18:13:29Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-dc5ff7a8069e4fb9a4feee34a70d82bc2023-11-20T07:56:45ZengMDPI AGInsects2075-44502020-07-0111847010.3390/insects11080470Effects of Temperature on Lifespan of <i>Drosophila melanogaster</i> from Different Genetic Backgrounds: Links between Metabolic Rate and LongevityMateusz Mołoń0Jan Dampc1Monika Kula-Maximenko2Jacek Zebrowski3Agnieszka Mołoń4Ralph Dobler5Roma Durak6Andrzej Skoczowski7Department of Biochemistry and Cell Biology, University of Rzeszow, 35-601 Rzeszów, PolandDepartment of Experimental Biology and Chemistry, University of Rzeszów, 35-310 Rzeszów, PolandThe Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 30-239 Krakow, PolandDepartment of Plant Physiology and Ecology, University of Rzeszow, 35-310 Rzeszów, PolandDepartment of Experimental Biology and Chemistry, University of Rzeszów, 35-310 Rzeszów, PolandApplied Zoology, Faculty of Biology, TU Dresden, 01062 Dresden, GermanyDepartment of Experimental Biology and Chemistry, University of Rzeszów, 35-310 Rzeszów, PolandInstitute of Biology, Pedagogical University of Krakow, 30-084 Krakow, PolandDespite many studies of the aging process, questions about key factors ensuring longevity have not yet found clear answers. Temperature seems to be one of the most important factors regulating lifespan. However, the genetic background may also play a key role in determining longevity. The aim of this study was to investigate the relationship between the temperature, genetic background (fruit fly origin), and metabolic rate on lifespan. Experiments were performed with the use of the wild type <i>Drosophila melanogaster</i> fruit flies originating from Australia, Canada, and Benin and the reference OregonR strain. The metabolic rate of <i>D. melanogaster</i> was measured at 20 °C, 25 °C, and 28 °C in an isothermal calorimeter. We found a strong negative relationship between the total heat flow and longevity. A high metabolic rate leads to increased aging in males and females in all strains. Furthermore, our results showed that temperature has a significant effect on fecundity and body weight. We also showed the usefulness of the isothermal calorimetry method to study the effect of environmental stress conditions on the metabolic activity of insects. This may be particularly important for the forecasting of impact of global warming on metabolic activity and lifespan of various insects.https://www.mdpi.com/2075-4450/11/8/470agingfruit flylifespanmicrocalorimetry |
| spellingShingle | Mateusz Mołoń Jan Dampc Monika Kula-Maximenko Jacek Zebrowski Agnieszka Mołoń Ralph Dobler Roma Durak Andrzej Skoczowski Effects of Temperature on Lifespan of <i>Drosophila melanogaster</i> from Different Genetic Backgrounds: Links between Metabolic Rate and Longevity Insects aging fruit fly lifespan microcalorimetry |
| title | Effects of Temperature on Lifespan of <i>Drosophila melanogaster</i> from Different Genetic Backgrounds: Links between Metabolic Rate and Longevity |
| title_full | Effects of Temperature on Lifespan of <i>Drosophila melanogaster</i> from Different Genetic Backgrounds: Links between Metabolic Rate and Longevity |
| title_fullStr | Effects of Temperature on Lifespan of <i>Drosophila melanogaster</i> from Different Genetic Backgrounds: Links between Metabolic Rate and Longevity |
| title_full_unstemmed | Effects of Temperature on Lifespan of <i>Drosophila melanogaster</i> from Different Genetic Backgrounds: Links between Metabolic Rate and Longevity |
| title_short | Effects of Temperature on Lifespan of <i>Drosophila melanogaster</i> from Different Genetic Backgrounds: Links between Metabolic Rate and Longevity |
| title_sort | effects of temperature on lifespan of i drosophila melanogaster i from different genetic backgrounds links between metabolic rate and longevity |
| topic | aging fruit fly lifespan microcalorimetry |
| url | https://www.mdpi.com/2075-4450/11/8/470 |
| work_keys_str_mv | AT mateuszmołon effectsoftemperatureonlifespanofidrosophilamelanogasterifromdifferentgeneticbackgroundslinksbetweenmetabolicrateandlongevity AT jandampc effectsoftemperatureonlifespanofidrosophilamelanogasterifromdifferentgeneticbackgroundslinksbetweenmetabolicrateandlongevity AT monikakulamaximenko effectsoftemperatureonlifespanofidrosophilamelanogasterifromdifferentgeneticbackgroundslinksbetweenmetabolicrateandlongevity AT jacekzebrowski effectsoftemperatureonlifespanofidrosophilamelanogasterifromdifferentgeneticbackgroundslinksbetweenmetabolicrateandlongevity AT agnieszkamołon effectsoftemperatureonlifespanofidrosophilamelanogasterifromdifferentgeneticbackgroundslinksbetweenmetabolicrateandlongevity AT ralphdobler effectsoftemperatureonlifespanofidrosophilamelanogasterifromdifferentgeneticbackgroundslinksbetweenmetabolicrateandlongevity AT romadurak effectsoftemperatureonlifespanofidrosophilamelanogasterifromdifferentgeneticbackgroundslinksbetweenmetabolicrateandlongevity AT andrzejskoczowski effectsoftemperatureonlifespanofidrosophilamelanogasterifromdifferentgeneticbackgroundslinksbetweenmetabolicrateandlongevity |