FORMATION OF TISSUE-ENGINEERED CONSTRUCT OF CARTILAGE IN VITRO
Aim. The goal of this study was to demonstrate the possibility of in vitro formation of tissue-engineered const- ruct of cartilage on the basis of cell-engineered construct composed of biopolymer hydrogel matrix and human adipose-derived mesenchymal stromal cells. Materials and methods. As a biodegr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2013-10-01
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| Series: | Вестник трансплантологии и искусственных органов |
| Subjects: | |
| Online Access: | https://journal.transpl.ru/vtio/article/view/432 |
| _version_ | 1826546451887423488 |
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| author | V. A. Surguchenko A. S. Ponomareva L. A. Kirsanova G. N. Bubentsova N. N. Skaletskij V. I. Sevastianov |
| author_facet | V. A. Surguchenko A. S. Ponomareva L. A. Kirsanova G. N. Bubentsova N. N. Skaletskij V. I. Sevastianov |
| author_sort | V. A. Surguchenko |
| collection | DOAJ |
| description | Aim. The goal of this study was to demonstrate the possibility of in vitro formation of tissue-engineered const- ruct of cartilage on the basis of cell-engineered construct composed of biopolymer hydrogel matrix and human adipose-derived mesenchymal stromal cells. Materials and methods. As a biodegradable matrix, the hetero- geneous composition of the implantable gel Sphero®GEL (Biomir Service, Krasnoznamensk, Moscow Region) was selected. To generate cell-engineered construct of cartilaginous tissue human adipose-derived mesenchy- mal stromal cells were mixed with biopolymer matrix Sphero®GEL and cultured in chondrogenesis medium. Results. It was revealed that human adipose-derived mesenchymal stromal cells with biopolymer matrix under chondrogenic conditions generate three-dimensional structures and produce cartilaginous extracellu- lar matrix components: collagen type II and glycosaminoglycans. Conclusion. It was discovered that 3-D structures formed in the process of culture of cell-engineered constructs, began to synthesize the components of the cartilage extracellular matrix. This indicates the initial stage of in vitro formation of tissue-engineered cartilage construct. |
| first_indexed | 2024-04-10T00:53:29Z |
| format | Article |
| id | doaj.art-dc61a8d7dfda4ec29d3b928e3740434c |
| institution | Directory Open Access Journal |
| issn | 1995-1191 |
| language | Russian |
| last_indexed | 2025-03-14T05:33:58Z |
| publishDate | 2013-10-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Вестник трансплантологии и искусственных органов |
| spelling | doaj.art-dc61a8d7dfda4ec29d3b928e3740434c2025-03-05T14:11:47ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovВестник трансплантологии и искусственных органов1995-11912013-10-01153667210.15825/1995-1191-2013-3-66-72375FORMATION OF TISSUE-ENGINEERED CONSTRUCT OF CARTILAGE IN VITROV. A. Surguchenko0A. S. Ponomareva1L. A. Kirsanova2G. N. Bubentsova3N. N. Skaletskij4V. I. Sevastianov5Laboratory of biomaterials and delivery systems (Head – doct. of biol. sci., prof. V.I. Sevastianov) Academician V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation Institute of Biomedical Research and Technology (ANO) (Head – doct. of biol. sci., prof. V.I. Sevastianov), Moscow, Russian FederationLaboratory of biomaterials and delivery systems (Head – doct. of biol. sci., prof. V.I. Sevastianov) Academician V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation Institute of Biomedical Research and Technology (ANO) (Head – doct. of biol. sci., prof. V.I. Sevastianov), Moscow, Russian FederationLaboratory of cell transplantation (Head – doct. of med. sci. N.N. Skaletskiy) Academician V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs, (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian FederationLaboratory of cell transplantation (Head – doct. of med. sci. N.N. Skaletskiy) Academician V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs, (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian FederationLaboratory of cell transplantation (Head – doct. of med. sci. N.N. Skaletskiy) Academician V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs, (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian FederationLaboratory of biomaterials and delivery systems (Head – doct. of biol. sci., prof. V.I. Sevastianov) Academician V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation Institute of Biomedical Research and Technology (ANO) (Head – doct. of biol. sci., prof. V.I. Sevastianov), Moscow, Russian FederationAim. The goal of this study was to demonstrate the possibility of in vitro formation of tissue-engineered const- ruct of cartilage on the basis of cell-engineered construct composed of biopolymer hydrogel matrix and human adipose-derived mesenchymal stromal cells. Materials and methods. As a biodegradable matrix, the hetero- geneous composition of the implantable gel Sphero®GEL (Biomir Service, Krasnoznamensk, Moscow Region) was selected. To generate cell-engineered construct of cartilaginous tissue human adipose-derived mesenchy- mal stromal cells were mixed with biopolymer matrix Sphero®GEL and cultured in chondrogenesis medium. Results. It was revealed that human adipose-derived mesenchymal stromal cells with biopolymer matrix under chondrogenic conditions generate three-dimensional structures and produce cartilaginous extracellu- lar matrix components: collagen type II and glycosaminoglycans. Conclusion. It was discovered that 3-D structures formed in the process of culture of cell-engineered constructs, began to synthesize the components of the cartilage extracellular matrix. This indicates the initial stage of in vitro formation of tissue-engineered cartilage construct.https://journal.transpl.ru/vtio/article/view/432biopolymer matrixhydrogelmesenchymal stromal cellsadipose tissuechondrogenic differentiationtissue-engineered construct. |
| spellingShingle | V. A. Surguchenko A. S. Ponomareva L. A. Kirsanova G. N. Bubentsova N. N. Skaletskij V. I. Sevastianov FORMATION OF TISSUE-ENGINEERED CONSTRUCT OF CARTILAGE IN VITRO Вестник трансплантологии и искусственных органов biopolymer matrix hydrogel mesenchymal stromal cells adipose tissue chondrogenic differentiation tissue-engineered construct. |
| title | FORMATION OF TISSUE-ENGINEERED CONSTRUCT OF CARTILAGE IN VITRO |
| title_full | FORMATION OF TISSUE-ENGINEERED CONSTRUCT OF CARTILAGE IN VITRO |
| title_fullStr | FORMATION OF TISSUE-ENGINEERED CONSTRUCT OF CARTILAGE IN VITRO |
| title_full_unstemmed | FORMATION OF TISSUE-ENGINEERED CONSTRUCT OF CARTILAGE IN VITRO |
| title_short | FORMATION OF TISSUE-ENGINEERED CONSTRUCT OF CARTILAGE IN VITRO |
| title_sort | formation of tissue engineered construct of cartilage in vitro |
| topic | biopolymer matrix hydrogel mesenchymal stromal cells adipose tissue chondrogenic differentiation tissue-engineered construct. |
| url | https://journal.transpl.ru/vtio/article/view/432 |
| work_keys_str_mv | AT vasurguchenko formationoftissueengineeredconstructofcartilageinvitro AT asponomareva formationoftissueengineeredconstructofcartilageinvitro AT lakirsanova formationoftissueengineeredconstructofcartilageinvitro AT gnbubentsova formationoftissueengineeredconstructofcartilageinvitro AT nnskaletskij formationoftissueengineeredconstructofcartilageinvitro AT visevastianov formationoftissueengineeredconstructofcartilageinvitro |