Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes
Covalent organic frameworks (COFs) are gaining growing interest owing to their various structures and versatility. Since their specific physical–chemical characteristics endow them great usage potentiality in biosensing, we herein have synthesized spherical COFs with regular shape and good dispersio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/9/704 |
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| author | Yuxin Lin Beibei Nie Xinyu Qu Minghui Wang Jie Yang Genxi Li |
| author_facet | Yuxin Lin Beibei Nie Xinyu Qu Minghui Wang Jie Yang Genxi Li |
| author_sort | Yuxin Lin |
| collection | DOAJ |
| description | Covalent organic frameworks (COFs) are gaining growing interest owing to their various structures and versatility. Since their specific physical–chemical characteristics endow them great usage potentiality in biosensing, we herein have synthesized spherical COFs with regular shape and good dispersion, which are further used for the design of a novel nanoprobe by modifying Histostar on the surface of the COFs. Moreover, we have applied a nanoprobe for the fabrication of an electrochemical biosensor to detect exosomes. Since Histostar is a special polymer, conjugated with many secondary antibodies (IgG), and HRP can increase the availability of HRP at the antigenic site, the biosensor can have a strong signal amplification ability. Meanwhile, since COFs with high porosity can be loaded with a huge amount of Histostar, the sensitivity of the biosensor can be further improved. With such a design, the proposed biosensor can achieve a low exosomes detection limit of 318 particles/µL, and a wide linear detection range from 10<sup>3</sup> particles/µL to 10<sup>8</sup> particles/µL. So, this work may offer a promising platform for the ultrasensitive detection of exosomes. |
| first_indexed | 2024-03-10T00:35:51Z |
| format | Article |
| id | doaj.art-dc660b7fb7d9417a8398e71083f7c649 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T00:35:51Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-dc660b7fb7d9417a8398e71083f7c6492023-11-23T15:17:44ZengMDPI AGBiosensors2079-63742022-09-0112970410.3390/bios12090704Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of ExosomesYuxin Lin0Beibei Nie1Xinyu Qu2Minghui Wang3Jie Yang4Genxi Li5State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing 210093, ChinaState Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing 210093, ChinaState Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing 210093, ChinaJiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Science, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing 210093, ChinaState Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing 210093, ChinaCovalent organic frameworks (COFs) are gaining growing interest owing to their various structures and versatility. Since their specific physical–chemical characteristics endow them great usage potentiality in biosensing, we herein have synthesized spherical COFs with regular shape and good dispersion, which are further used for the design of a novel nanoprobe by modifying Histostar on the surface of the COFs. Moreover, we have applied a nanoprobe for the fabrication of an electrochemical biosensor to detect exosomes. Since Histostar is a special polymer, conjugated with many secondary antibodies (IgG), and HRP can increase the availability of HRP at the antigenic site, the biosensor can have a strong signal amplification ability. Meanwhile, since COFs with high porosity can be loaded with a huge amount of Histostar, the sensitivity of the biosensor can be further improved. With such a design, the proposed biosensor can achieve a low exosomes detection limit of 318 particles/µL, and a wide linear detection range from 10<sup>3</sup> particles/µL to 10<sup>8</sup> particles/µL. So, this work may offer a promising platform for the ultrasensitive detection of exosomes.https://www.mdpi.com/2079-6374/12/9/704covalent organic frameworksHistostarexosomeelectrochemical biosensor |
| spellingShingle | Yuxin Lin Beibei Nie Xinyu Qu Minghui Wang Jie Yang Genxi Li Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes Biosensors covalent organic frameworks Histostar exosome electrochemical biosensor |
| title | Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes |
| title_full | Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes |
| title_fullStr | Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes |
| title_full_unstemmed | Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes |
| title_short | Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes |
| title_sort | histostar functionalized covalent organic framework for electrochemical detection of exosomes |
| topic | covalent organic frameworks Histostar exosome electrochemical biosensor |
| url | https://www.mdpi.com/2079-6374/12/9/704 |
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