A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry
The recent outbreak of the coronavirus disease 2019 (COVID-19) pandemic and the continuous evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have highlighted the significance of new detection methods for global monitoring and prevention. Although quantitative reverse tran...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Biosafety and Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590053623000216 |
| _version_ | 1797836780617072640 |
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| author | Ziyuan Zhao Liying Sun Liqin Wang Xiaodong Li Junping Peng |
| author_facet | Ziyuan Zhao Liying Sun Liqin Wang Xiaodong Li Junping Peng |
| author_sort | Ziyuan Zhao |
| collection | DOAJ |
| description | The recent outbreak of the coronavirus disease 2019 (COVID-19) pandemic and the continuous evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have highlighted the significance of new detection methods for global monitoring and prevention. Although quantitative reverse transcription PCR (RT-qPCR), the current gold standard for diagnosis, performs excellently in genetic testing, its multiplexing capability is limited because of the signal crosstalk of various fluorophores. Herein, we present a highly efficient platform which combines 17-plex assays with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), enabling the targeting of 14 different mutation sites of the spike gene. Diagnosis using a set of 324 nasopharyngeal swabs or sputum clinical samples with SARS-CoV-2 MS method was identical to that with the RT-qPCR. The detection consistency of mutation sites was 97.9% (47/48) compared to Sanger sequencing without cross-reaction with other respiratory-related pathogens. Therefore, the MS method is highly potent to track and assess SARS-CoV-2 changes in a timely manner, thereby aiding the continuous response to viral variation and prevention of further transmission. |
| first_indexed | 2024-04-09T15:14:25Z |
| format | Article |
| id | doaj.art-dc68af5a72e54e3bbd008ad262068929 |
| institution | Directory Open Access Journal |
| issn | 2590-0536 |
| language | English |
| last_indexed | 2024-04-09T15:14:25Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosafety and Health |
| spelling | doaj.art-dc68af5a72e54e3bbd008ad2620689292023-04-30T06:09:11ZengElsevierBiosafety and Health2590-05362023-04-0152101107A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometryZiyuan Zhao0Liying Sun1Liqin Wang2Xiaodong Li3Junping Peng4NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China; Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, ChinaNHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China; Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, ChinaNHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China; Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, ChinaShimadzu China Innovation Center, Shimadzu Corporation, Beijing 100020, ChinaNHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China; Key Laboratory of Respiratory Disease Pathogenomics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China; Corresponding author: NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China (J. Peng).The recent outbreak of the coronavirus disease 2019 (COVID-19) pandemic and the continuous evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have highlighted the significance of new detection methods for global monitoring and prevention. Although quantitative reverse transcription PCR (RT-qPCR), the current gold standard for diagnosis, performs excellently in genetic testing, its multiplexing capability is limited because of the signal crosstalk of various fluorophores. Herein, we present a highly efficient platform which combines 17-plex assays with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), enabling the targeting of 14 different mutation sites of the spike gene. Diagnosis using a set of 324 nasopharyngeal swabs or sputum clinical samples with SARS-CoV-2 MS method was identical to that with the RT-qPCR. The detection consistency of mutation sites was 97.9% (47/48) compared to Sanger sequencing without cross-reaction with other respiratory-related pathogens. Therefore, the MS method is highly potent to track and assess SARS-CoV-2 changes in a timely manner, thereby aiding the continuous response to viral variation and prevention of further transmission.http://www.sciencedirect.com/science/article/pii/S2590053623000216COVID-19SARS-CoV-2Multiplexing capabilityMALDI-TOF mass spectrometryViral variation |
| spellingShingle | Ziyuan Zhao Liying Sun Liqin Wang Xiaodong Li Junping Peng A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry Biosafety and Health COVID-19 SARS-CoV-2 Multiplexing capability MALDI-TOF mass spectrometry Viral variation |
| title | A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry |
| title_full | A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry |
| title_fullStr | A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry |
| title_full_unstemmed | A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry |
| title_short | A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry |
| title_sort | multiplex method for detection of sars cov 2 variants based on maldi tof mass spectrometry |
| topic | COVID-19 SARS-CoV-2 Multiplexing capability MALDI-TOF mass spectrometry Viral variation |
| url | http://www.sciencedirect.com/science/article/pii/S2590053623000216 |
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