Analysis of the Genetic Structure of Slovak Holstein Cattle Using Seven Candidate Genes Related to Milk Quality
Milk is an important component of human nutrition, and its composition and milk yield fundamentally affect the economy of dairy farms. Genetic variability is a fundamental premise for livestock breeding and is commonly used in the identification of individual animals and in selection to improve perf...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/1424-2818/14/11/989 |
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| author | Martina Miluchová Michal Gábor Juraj Gašper |
| author_facet | Martina Miluchová Michal Gábor Juraj Gašper |
| author_sort | Martina Miluchová |
| collection | DOAJ |
| description | Milk is an important component of human nutrition, and its composition and milk yield fundamentally affect the economy of dairy farms. Genetic variability is a fundamental premise for livestock breeding and is commonly used in the identification of individual animals and in selection to improve performance. The aims of this study were to propose a rapid detection method for genes affecting the nutritional value and technological properties of bovine milk (<i>FADS1</i>, <i>FADS2</i>, <i>FASN</i>, <i>SCD</i>, <i>DGAT1</i>, <i>CSN2</i> and <i>CSN3</i>) and to analyze Slovak Holstein cattle to widen knowledge on their genetic structure for these candidate genes. Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and artificially created restriction site-polymerase chain reaction (ACRS-PCR) methods. Heterozygosity is an important factor for estimating genetic variation in domestic animals and represents the genetic potential and ability to adapt to the natural environment. In this study, Holstein cattle showed high heterozygosity values for markers <i>FADS1-07</i> and <i>CSN2-H67P</i>. In contrast, they showed high homozygosity values for markers <i>FADS1-01</i>, <i>FADS2-23</i>, <i>FASN-16024</i>, <i>SCD-T878C</i>, <i>DGAT1-K232A</i> and <i>CSN3-D148A</i>. These results suggest that genetic diversity has been reduced, which may be due to breeding effects. |
| first_indexed | 2024-03-09T18:23:54Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-09T18:23:54Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
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| series | Diversity |
| spelling | doaj.art-dc6d6aa5788f49c89c5f8f040b4e58032023-11-24T08:06:21ZengMDPI AGDiversity1424-28182022-11-01141198910.3390/d14110989Analysis of the Genetic Structure of Slovak Holstein Cattle Using Seven Candidate Genes Related to Milk QualityMartina Miluchová0Michal Gábor1Juraj Gašper2Institute of Nutrition and Genomics, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Nutrition and Genomics, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute of Nutrition and Genomics, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaMilk is an important component of human nutrition, and its composition and milk yield fundamentally affect the economy of dairy farms. Genetic variability is a fundamental premise for livestock breeding and is commonly used in the identification of individual animals and in selection to improve performance. The aims of this study were to propose a rapid detection method for genes affecting the nutritional value and technological properties of bovine milk (<i>FADS1</i>, <i>FADS2</i>, <i>FASN</i>, <i>SCD</i>, <i>DGAT1</i>, <i>CSN2</i> and <i>CSN3</i>) and to analyze Slovak Holstein cattle to widen knowledge on their genetic structure for these candidate genes. Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and artificially created restriction site-polymerase chain reaction (ACRS-PCR) methods. Heterozygosity is an important factor for estimating genetic variation in domestic animals and represents the genetic potential and ability to adapt to the natural environment. In this study, Holstein cattle showed high heterozygosity values for markers <i>FADS1-07</i> and <i>CSN2-H67P</i>. In contrast, they showed high homozygosity values for markers <i>FADS1-01</i>, <i>FADS2-23</i>, <i>FASN-16024</i>, <i>SCD-T878C</i>, <i>DGAT1-K232A</i> and <i>CSN3-D148A</i>. These results suggest that genetic diversity has been reduced, which may be due to breeding effects.https://www.mdpi.com/1424-2818/14/11/989genetic structureheterozygositymilkHolstein cattle |
| spellingShingle | Martina Miluchová Michal Gábor Juraj Gašper Analysis of the Genetic Structure of Slovak Holstein Cattle Using Seven Candidate Genes Related to Milk Quality Diversity genetic structure heterozygosity milk Holstein cattle |
| title | Analysis of the Genetic Structure of Slovak Holstein Cattle Using Seven Candidate Genes Related to Milk Quality |
| title_full | Analysis of the Genetic Structure of Slovak Holstein Cattle Using Seven Candidate Genes Related to Milk Quality |
| title_fullStr | Analysis of the Genetic Structure of Slovak Holstein Cattle Using Seven Candidate Genes Related to Milk Quality |
| title_full_unstemmed | Analysis of the Genetic Structure of Slovak Holstein Cattle Using Seven Candidate Genes Related to Milk Quality |
| title_short | Analysis of the Genetic Structure of Slovak Holstein Cattle Using Seven Candidate Genes Related to Milk Quality |
| title_sort | analysis of the genetic structure of slovak holstein cattle using seven candidate genes related to milk quality |
| topic | genetic structure heterozygosity milk Holstein cattle |
| url | https://www.mdpi.com/1424-2818/14/11/989 |
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