Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age
Abstract Neonatal calves possess a very immature and naïve immune system and are reliant on the intake of maternal colostrum for passive transfer of immunoglobulins. Variation in colostrum management of beef and dairy calves is thought to affect early immune development. Therefore, the objective of...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2018-10-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-018-33627-0 |
| _version_ | 1818998134254075904 |
|---|---|
| author | C. Surlis B. Earley M. McGee K. Keogh P. Cormican G. Blackshields K. Tiernan A. Dunn S. Morrison A. Arguello S. M. Waters |
| author_facet | C. Surlis B. Earley M. McGee K. Keogh P. Cormican G. Blackshields K. Tiernan A. Dunn S. Morrison A. Arguello S. M. Waters |
| author_sort | C. Surlis |
| collection | DOAJ |
| description | Abstract Neonatal calves possess a very immature and naïve immune system and are reliant on the intake of maternal colostrum for passive transfer of immunoglobulins. Variation in colostrum management of beef and dairy calves is thought to affect early immune development. Therefore, the objective of this study was to examine changes in gene expression and investigate molecular pathways involved in the immune-competence development of neonatal Holstein dairy calves and naturally suckled beef calves using next generation RNA-sequencing during the first week of life. Jugular whole blood samples were collected from Holstein (H) dairy calves (n = 8) artificially fed 5% B.W. colostrum, and from beef calves which were the progenies of Charolais-Limousin (CL; n = 7) and Limousin-Friesian beef suckler cows (LF; n = 7), for subsequent RNA isolation. In dairy calves, there was a surge in pro-inflammatory cytokine gene expression possibly due to the stress of separation from the dam. LF calves exhibited early signs of humoral immune development with observed increases in the expression genes coding for Ig receptors, which was not evident in the other breeds by 7 days of age. Immune and health related DEGs identified as upregulated in beef calves are prospective contender genes for the classification of biomarkers for immune-competence development, and will contribute towards a greater understanding of the development of an immune response in neonatal calves. |
| first_indexed | 2024-12-20T21:56:42Z |
| format | Article |
| id | doaj.art-dc6e8f152c67440d8d4140385216ed88 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:56:42Z |
| publishDate | 2018-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-dc6e8f152c67440d8d4140385216ed882022-12-21T19:25:27ZengNature PortfolioScientific Reports2045-23222018-10-018111110.1038/s41598-018-33627-0Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of ageC. Surlis0B. Earley1M. McGee2K. Keogh3P. Cormican4G. Blackshields5K. Tiernan6A. Dunn7S. Morrison8A. Arguello9S. M. Waters10Teagasc Animal and Bioscience Research Department, Grange, DunsanyTeagasc Animal and Bioscience Research Department, Grange, DunsanyTeagasc Animal and Bioscience Research Department, Grange, DunsanyTeagasc Animal and Bioscience Research Department, Grange, DunsanyTeagasc Animal and Bioscience Research Department, Grange, DunsanyTeagasc Animal and Bioscience Research Department, Grange, DunsanyTeagasc Animal and Bioscience Research Department, Grange, DunsanySustainable Livestock, Agri-food and Bio-sciences InstituteSustainable Livestock, Agri-food and Bio-sciences InstituteTeagasc Animal and Bioscience Research Department, Grange, DunsanyTeagasc Animal and Bioscience Research Department, Grange, DunsanyAbstract Neonatal calves possess a very immature and naïve immune system and are reliant on the intake of maternal colostrum for passive transfer of immunoglobulins. Variation in colostrum management of beef and dairy calves is thought to affect early immune development. Therefore, the objective of this study was to examine changes in gene expression and investigate molecular pathways involved in the immune-competence development of neonatal Holstein dairy calves and naturally suckled beef calves using next generation RNA-sequencing during the first week of life. Jugular whole blood samples were collected from Holstein (H) dairy calves (n = 8) artificially fed 5% B.W. colostrum, and from beef calves which were the progenies of Charolais-Limousin (CL; n = 7) and Limousin-Friesian beef suckler cows (LF; n = 7), for subsequent RNA isolation. In dairy calves, there was a surge in pro-inflammatory cytokine gene expression possibly due to the stress of separation from the dam. LF calves exhibited early signs of humoral immune development with observed increases in the expression genes coding for Ig receptors, which was not evident in the other breeds by 7 days of age. Immune and health related DEGs identified as upregulated in beef calves are prospective contender genes for the classification of biomarkers for immune-competence development, and will contribute towards a greater understanding of the development of an immune response in neonatal calves.https://doi.org/10.1038/s41598-018-33627-0Dairy CalvesBeef CalvesBeef SucklerColostrum ManagementDifferentially Expressed Genes (DEG) |
| spellingShingle | C. Surlis B. Earley M. McGee K. Keogh P. Cormican G. Blackshields K. Tiernan A. Dunn S. Morrison A. Arguello S. M. Waters Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age Scientific Reports Dairy Calves Beef Calves Beef Suckler Colostrum Management Differentially Expressed Genes (DEG) |
| title | Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age |
| title_full | Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age |
| title_fullStr | Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age |
| title_full_unstemmed | Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age |
| title_short | Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age |
| title_sort | blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age |
| topic | Dairy Calves Beef Calves Beef Suckler Colostrum Management Differentially Expressed Genes (DEG) |
| url | https://doi.org/10.1038/s41598-018-33627-0 |
| work_keys_str_mv | AT csurlis bloodimmunetranscriptomeanalysisofartificiallyfeddairycalvesandnaturallysuckledbeefcalvesfrombirthto7daysofage AT bearley bloodimmunetranscriptomeanalysisofartificiallyfeddairycalvesandnaturallysuckledbeefcalvesfrombirthto7daysofage AT mmcgee bloodimmunetranscriptomeanalysisofartificiallyfeddairycalvesandnaturallysuckledbeefcalvesfrombirthto7daysofage AT kkeogh bloodimmunetranscriptomeanalysisofartificiallyfeddairycalvesandnaturallysuckledbeefcalvesfrombirthto7daysofage AT pcormican bloodimmunetranscriptomeanalysisofartificiallyfeddairycalvesandnaturallysuckledbeefcalvesfrombirthto7daysofage AT gblackshields bloodimmunetranscriptomeanalysisofartificiallyfeddairycalvesandnaturallysuckledbeefcalvesfrombirthto7daysofage AT ktiernan bloodimmunetranscriptomeanalysisofartificiallyfeddairycalvesandnaturallysuckledbeefcalvesfrombirthto7daysofage AT adunn bloodimmunetranscriptomeanalysisofartificiallyfeddairycalvesandnaturallysuckledbeefcalvesfrombirthto7daysofage AT smorrison bloodimmunetranscriptomeanalysisofartificiallyfeddairycalvesandnaturallysuckledbeefcalvesfrombirthto7daysofage AT aarguello bloodimmunetranscriptomeanalysisofartificiallyfeddairycalvesandnaturallysuckledbeefcalvesfrombirthto7daysofage AT smwaters bloodimmunetranscriptomeanalysisofartificiallyfeddairycalvesandnaturallysuckledbeefcalvesfrombirthto7daysofage |