Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice
IntroductionObesity and diabetes are common chronic metabolic disorders which can cause an imbalance of the intestinal flora and gut-liver metabolism. Several studies have shown that probiotics, including Escherichia coli Nissle 1917 (EcN), promote microbial balance and metabolic health. However, th...
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1219763/full |
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| author | Jun Shi Jun Shi DongXue Ma DongXue Ma ShanHu Gao ShanHu Gao Fei Long Fei Long Xin Wang Xin Wang XingYu Pu XingYu Pu Richard D. Cannon Ting-Li Han Ting-Li Han Ting-Li Han |
| author_facet | Jun Shi Jun Shi DongXue Ma DongXue Ma ShanHu Gao ShanHu Gao Fei Long Fei Long Xin Wang Xin Wang XingYu Pu XingYu Pu Richard D. Cannon Ting-Li Han Ting-Li Han Ting-Li Han |
| author_sort | Jun Shi |
| collection | DOAJ |
| description | IntroductionObesity and diabetes are common chronic metabolic disorders which can cause an imbalance of the intestinal flora and gut-liver metabolism. Several studies have shown that probiotics, including Escherichia coli Nissle 1917 (EcN), promote microbial balance and metabolic health. However, there are no studies on how EcN outer membrane vesicles (EcN-OMVs) influence the intestinal microflora and affect the metabolic disorders of obesity and diabetes.MethodsIn this study, we evaluated the effects of EcN-OMVs on high-fat diet (HFD)-induced obesity and HFD + streptozotocin (STZ)-induced diabetes.ResultsEcN-OMVs could reduce body weight, decrease blood glucose, and increase plasma insulin in obese mice. Similarly, EcN-OMVs treatment could modify the ratio of Firmicutes/Bacteroidetes in the gut, elevate intestinal short-chain fatty acid (SCFA)-producing flora, and influence the SCFA content of the intestine. Furthermore, the intestinal metabolites ornithine and fumaric acid, hepatic ω-6 unsaturated fatty acids, and SCFAs were significantly increased after administering EcN-OMVs.DiscussionOverall, this study showed that EcN-OMVs might act as post-biotic agents that could modulate gut-liver metabolism and ameliorate the pathophysiology of obesity and diabetes. |
| first_indexed | 2024-03-12T14:38:35Z |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T14:38:35Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-dc6e972f964b46e6b7202630cf0aff2a2023-08-17T00:51:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12197631219763Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic miceJun Shi0Jun Shi1DongXue Ma2DongXue Ma3ShanHu Gao4ShanHu Gao5Fei Long6Fei Long7Xin Wang8Xin Wang9XingYu Pu10XingYu Pu11Richard D. Cannon12Ting-Li Han13Ting-Li Han14Ting-Li Han15State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaState Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaState Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaState Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaState Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaState Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaDepartment of Oral Sciences, Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New ZealandState Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaChongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, ChinaDepartment of Obstetrics and Gynaecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaIntroductionObesity and diabetes are common chronic metabolic disorders which can cause an imbalance of the intestinal flora and gut-liver metabolism. Several studies have shown that probiotics, including Escherichia coli Nissle 1917 (EcN), promote microbial balance and metabolic health. However, there are no studies on how EcN outer membrane vesicles (EcN-OMVs) influence the intestinal microflora and affect the metabolic disorders of obesity and diabetes.MethodsIn this study, we evaluated the effects of EcN-OMVs on high-fat diet (HFD)-induced obesity and HFD + streptozotocin (STZ)-induced diabetes.ResultsEcN-OMVs could reduce body weight, decrease blood glucose, and increase plasma insulin in obese mice. Similarly, EcN-OMVs treatment could modify the ratio of Firmicutes/Bacteroidetes in the gut, elevate intestinal short-chain fatty acid (SCFA)-producing flora, and influence the SCFA content of the intestine. Furthermore, the intestinal metabolites ornithine and fumaric acid, hepatic ω-6 unsaturated fatty acids, and SCFAs were significantly increased after administering EcN-OMVs.DiscussionOverall, this study showed that EcN-OMVs might act as post-biotic agents that could modulate gut-liver metabolism and ameliorate the pathophysiology of obesity and diabetes.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1219763/fullprobioticsEscherichia coli Nissle 1917outer membrane vesiclesdiabetesobesitygut microbiota |
| spellingShingle | Jun Shi Jun Shi DongXue Ma DongXue Ma ShanHu Gao ShanHu Gao Fei Long Fei Long Xin Wang Xin Wang XingYu Pu XingYu Pu Richard D. Cannon Ting-Li Han Ting-Li Han Ting-Li Han Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice Frontiers in Microbiology probiotics Escherichia coli Nissle 1917 outer membrane vesicles diabetes obesity gut microbiota |
| title | Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice |
| title_full | Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice |
| title_fullStr | Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice |
| title_full_unstemmed | Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice |
| title_short | Probiotic Escherichia coli Nissle 1917-derived outer membrane vesicles modulate the intestinal microbiome and host gut-liver metabolome in obese and diabetic mice |
| title_sort | probiotic escherichia coli nissle 1917 derived outer membrane vesicles modulate the intestinal microbiome and host gut liver metabolome in obese and diabetic mice |
| topic | probiotics Escherichia coli Nissle 1917 outer membrane vesicles diabetes obesity gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1219763/full |
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