CONSTRUCTION OF A DNA-MICROARRAY FOR DIFFERENTIATION BETWEEN THE MAIN AND NON-MAIN SUBSPECIES AND BIOVARS OF THE MAIN SUBSPECIES OF YERSINIA PESTIS
Objective of the study is to design the DNA-microarray for differentiation of Y. pestis strains of the main and non-main subspecies and biovars of the main subspecies. Materials and methods. Efficiency analysis for the devised means was conducted using 62 Y. pestis strains of various subspecies and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2017-06-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/387 |
| _version_ | 1797220834039824384 |
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| author | K. A. Nikiforov D. V. Utkin L. M. Kukleva G. A. Eroshenko |
| author_facet | K. A. Nikiforov D. V. Utkin L. M. Kukleva G. A. Eroshenko |
| author_sort | K. A. Nikiforov |
| collection | DOAJ |
| description | Objective of the study is to design the DNA-microarray for differentiation of Y. pestis strains of the main and non-main subspecies and biovars of the main subspecies. Materials and methods. Efficiency analysis for the devised means was conducted using 62 Y. pestis strains of various subspecies and biovars, isolated in the natural foci of Russia and neighboring countries. Results and conclusions. Selected have been the DNA-targets, probes and primers – calculated. Enhanced is the method of sub-specific and biovar differentiation of Y. pestis strains by means of DNA-microarray. DNA-chip with “Med24”, “glpD(-93)”, and “45” targets allows for prompt differentiation of the strains of the main and non-main subspecies and biovars of the main subspecies based on the presence and absence of fluorescent signal by the specific for the main subspecies and its biovars DNA-targets. |
| first_indexed | 2024-03-08T13:42:55Z |
| format | Article |
| id | doaj.art-dc706ea86d5946a99b13f991eabddc0c |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:55:50Z |
| publishDate | 2017-06-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-dc706ea86d5946a99b13f991eabddc0c2024-04-05T16:47:12ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2017-06-0102323510.21055/0370-1069-2017-2-32-35387CONSTRUCTION OF A DNA-MICROARRAY FOR DIFFERENTIATION BETWEEN THE MAIN AND NON-MAIN SUBSPECIES AND BIOVARS OF THE MAIN SUBSPECIES OF YERSINIA PESTISK. A. Nikiforov0D. V. Utkin1L. M. Kukleva2G. A. Eroshenko3Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Objective of the study is to design the DNA-microarray for differentiation of Y. pestis strains of the main and non-main subspecies and biovars of the main subspecies. Materials and methods. Efficiency analysis for the devised means was conducted using 62 Y. pestis strains of various subspecies and biovars, isolated in the natural foci of Russia and neighboring countries. Results and conclusions. Selected have been the DNA-targets, probes and primers – calculated. Enhanced is the method of sub-specific and biovar differentiation of Y. pestis strains by means of DNA-microarray. DNA-chip with “Med24”, “glpD(-93)”, and “45” targets allows for prompt differentiation of the strains of the main and non-main subspecies and biovars of the main subspecies based on the presence and absence of fluorescent signal by the specific for the main subspecies and its biovars DNA-targets.https://journal.microbe.ru/jour/article/view/387dna-microarraydna-targetbiovary. pestis |
| spellingShingle | K. A. Nikiforov D. V. Utkin L. M. Kukleva G. A. Eroshenko CONSTRUCTION OF A DNA-MICROARRAY FOR DIFFERENTIATION BETWEEN THE MAIN AND NON-MAIN SUBSPECIES AND BIOVARS OF THE MAIN SUBSPECIES OF YERSINIA PESTIS Проблемы особо опасных инфекций dna-microarray dna-target biovar y. pestis |
| title | CONSTRUCTION OF A DNA-MICROARRAY FOR DIFFERENTIATION BETWEEN THE MAIN AND NON-MAIN SUBSPECIES AND BIOVARS OF THE MAIN SUBSPECIES OF YERSINIA PESTIS |
| title_full | CONSTRUCTION OF A DNA-MICROARRAY FOR DIFFERENTIATION BETWEEN THE MAIN AND NON-MAIN SUBSPECIES AND BIOVARS OF THE MAIN SUBSPECIES OF YERSINIA PESTIS |
| title_fullStr | CONSTRUCTION OF A DNA-MICROARRAY FOR DIFFERENTIATION BETWEEN THE MAIN AND NON-MAIN SUBSPECIES AND BIOVARS OF THE MAIN SUBSPECIES OF YERSINIA PESTIS |
| title_full_unstemmed | CONSTRUCTION OF A DNA-MICROARRAY FOR DIFFERENTIATION BETWEEN THE MAIN AND NON-MAIN SUBSPECIES AND BIOVARS OF THE MAIN SUBSPECIES OF YERSINIA PESTIS |
| title_short | CONSTRUCTION OF A DNA-MICROARRAY FOR DIFFERENTIATION BETWEEN THE MAIN AND NON-MAIN SUBSPECIES AND BIOVARS OF THE MAIN SUBSPECIES OF YERSINIA PESTIS |
| title_sort | construction of a dna microarray for differentiation between the main and non main subspecies and biovars of the main subspecies of yersinia pestis |
| topic | dna-microarray dna-target biovar y. pestis |
| url | https://journal.microbe.ru/jour/article/view/387 |
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