Mycobiome changes in the vitreous of post fever retinitis patients.
Fungi have been associated with various diseases of the eye like keratitis, uveitis and endophthalmitis. Despite this fact, fungal microbiome (mycobiome) studies compared to the bacterial microbiome studies have remained neglected. In the present study, using metagenomic sequencing, the mycobiomes o...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0242138 |
| _version_ | 1819292821644902400 |
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| author | Kotakonda Arunasri Malleswarapu Mahesh Gumpili Sai Prashanthi Rajagopalaboopathi Jayasudha Sama Kalyana Chakravarthy Mudit Tyagi Rajeev R Pappuru Sisinthy Shivaji |
| author_facet | Kotakonda Arunasri Malleswarapu Mahesh Gumpili Sai Prashanthi Rajagopalaboopathi Jayasudha Sama Kalyana Chakravarthy Mudit Tyagi Rajeev R Pappuru Sisinthy Shivaji |
| author_sort | Kotakonda Arunasri |
| collection | DOAJ |
| description | Fungi have been associated with various diseases of the eye like keratitis, uveitis and endophthalmitis. Despite this fact, fungal microbiome (mycobiome) studies compared to the bacterial microbiome studies have remained neglected. In the present study, using metagenomic sequencing, the mycobiomes of the vitreous of healthy control individuals (VC, n = 15) and individuals with post fever retinitis + non-PFR uveitis (PFR+, n = 9) were analysed and compared. The results indicated that Ascomycota was the most predominant phylum in both VC and PFR+ groups. Further, at the genera level it was observed that the abundance of 17 fungal genera were significantly different in post fever retinitis (PFR, n = 6) group compared to control group. Of these 17 genera, it was observed that 14 genera were relatively more abundant in PFR group and the remaining 3 genera in the VC group. Genus Saccharomyces, a commensal of the gut and skin, was predominantly present in the vitreous of both the cohorts, however it was significantly less abundant in PFR group. Further, significant increase in the genera that have a pathogenic interaction with the host were observed in PFR group. On the whole the mycobiome in both the groups differed significantly and formed two distinct clusters in the heatmap and Principal co-ordinate analysis. These results demonstrate significant changes in the mycobiome from the vitreous of post fever retinitis patients compared to healthy controls thus implying that dysbiotic changes in the fungal vitreous microbiome are associated with PFR. |
| first_indexed | 2024-12-24T04:00:38Z |
| format | Article |
| id | doaj.art-dc71f888e1fd43a89c8382434093cd69 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-24T04:00:38Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-dc71f888e1fd43a89c8382434093cd692022-12-21T17:16:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011511e024213810.1371/journal.pone.0242138Mycobiome changes in the vitreous of post fever retinitis patients.Kotakonda ArunasriMalleswarapu MaheshGumpili Sai PrashanthiRajagopalaboopathi JayasudhaSama Kalyana ChakravarthyMudit TyagiRajeev R PappuruSisinthy ShivajiFungi have been associated with various diseases of the eye like keratitis, uveitis and endophthalmitis. Despite this fact, fungal microbiome (mycobiome) studies compared to the bacterial microbiome studies have remained neglected. In the present study, using metagenomic sequencing, the mycobiomes of the vitreous of healthy control individuals (VC, n = 15) and individuals with post fever retinitis + non-PFR uveitis (PFR+, n = 9) were analysed and compared. The results indicated that Ascomycota was the most predominant phylum in both VC and PFR+ groups. Further, at the genera level it was observed that the abundance of 17 fungal genera were significantly different in post fever retinitis (PFR, n = 6) group compared to control group. Of these 17 genera, it was observed that 14 genera were relatively more abundant in PFR group and the remaining 3 genera in the VC group. Genus Saccharomyces, a commensal of the gut and skin, was predominantly present in the vitreous of both the cohorts, however it was significantly less abundant in PFR group. Further, significant increase in the genera that have a pathogenic interaction with the host were observed in PFR group. On the whole the mycobiome in both the groups differed significantly and formed two distinct clusters in the heatmap and Principal co-ordinate analysis. These results demonstrate significant changes in the mycobiome from the vitreous of post fever retinitis patients compared to healthy controls thus implying that dysbiotic changes in the fungal vitreous microbiome are associated with PFR.https://doi.org/10.1371/journal.pone.0242138 |
| spellingShingle | Kotakonda Arunasri Malleswarapu Mahesh Gumpili Sai Prashanthi Rajagopalaboopathi Jayasudha Sama Kalyana Chakravarthy Mudit Tyagi Rajeev R Pappuru Sisinthy Shivaji Mycobiome changes in the vitreous of post fever retinitis patients. PLoS ONE |
| title | Mycobiome changes in the vitreous of post fever retinitis patients. |
| title_full | Mycobiome changes in the vitreous of post fever retinitis patients. |
| title_fullStr | Mycobiome changes in the vitreous of post fever retinitis patients. |
| title_full_unstemmed | Mycobiome changes in the vitreous of post fever retinitis patients. |
| title_short | Mycobiome changes in the vitreous of post fever retinitis patients. |
| title_sort | mycobiome changes in the vitreous of post fever retinitis patients |
| url | https://doi.org/10.1371/journal.pone.0242138 |
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