miRNA-126a plays important role in myoblast and endothelial cell interaction
Abstract Muscle satellite cells (SCs) are stem cells and the main players in skeletal muscle reconstruction. Since satellite cells are located near or in direct contact with blood vessels their niche is formed, inter alia, by endothelial cells. The cross-talk between satellite cells and endothelial...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-09-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-41626-z |
| _version_ | 1827634494588321792 |
|---|---|
| author | Bartosz Mierzejewski Maria Anna Ciemerych Wladyslawa Streminska Katarzyna Janczyk-Ilach Edyta Brzoska |
| author_facet | Bartosz Mierzejewski Maria Anna Ciemerych Wladyslawa Streminska Katarzyna Janczyk-Ilach Edyta Brzoska |
| author_sort | Bartosz Mierzejewski |
| collection | DOAJ |
| description | Abstract Muscle satellite cells (SCs) are stem cells and the main players in skeletal muscle reconstruction. Since satellite cells are located near or in direct contact with blood vessels their niche is formed, inter alia, by endothelial cells. The cross-talk between satellite cells and endothelial cells determines quiescence or proliferation of these cells. However, little is known about the role of miRNA in these interactions. In the present study we identified miRNA that were up-regulated in SC-derived myoblasts treated with stromal derived factor-1 (SDF-1) and/or down-regulated in cells in which the expression of CXCR4 or CXCR7, that is, SDF-1 receptors, was silenced. SDF-1 is one of the important regulators of cell migration, mobilization, skeletal muscle regeneration, and angiogenesis. We hypothesized that selected miRNAs affect SC-derived myoblast fate and interactions with endothelial cells. We showed that miR-126a-3p inhibited both, myoblast migration and fusion. Moreover, the levels of Cxcl12, encoding SDF-1 and Ackr3, encoding CXCR7, were reduced by miR-126a-3p mimic. Interestingly, the miR-126a-3p mimic significantly decreased the level of numerous factors involved in myogenesis and the miR-126a-5p mimic increased the level of Vefga. Importantly, the treatment of endothelial cells with medium conditioned by miR-126-5p mimic transfected SC-derived myoblasts promoted tubulogenesis. |
| first_indexed | 2024-03-09T15:14:09Z |
| format | Article |
| id | doaj.art-dc7572e1af474fbea35d591ceefef4e5 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:14:09Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-dc7572e1af474fbea35d591ceefef4e52023-11-26T13:12:44ZengNature PortfolioScientific Reports2045-23222023-09-0113111510.1038/s41598-023-41626-zmiRNA-126a plays important role in myoblast and endothelial cell interactionBartosz Mierzejewski0Maria Anna Ciemerych1Wladyslawa Streminska2Katarzyna Janczyk-Ilach3Edyta Brzoska4Department of Cytology, Faculty of Biology, University of WarsawDepartment of Cytology, Faculty of Biology, University of WarsawDepartment of Cytology, Faculty of Biology, University of WarsawDepartment of Cytology, Faculty of Biology, University of WarsawDepartment of Cytology, Faculty of Biology, University of WarsawAbstract Muscle satellite cells (SCs) are stem cells and the main players in skeletal muscle reconstruction. Since satellite cells are located near or in direct contact with blood vessels their niche is formed, inter alia, by endothelial cells. The cross-talk between satellite cells and endothelial cells determines quiescence or proliferation of these cells. However, little is known about the role of miRNA in these interactions. In the present study we identified miRNA that were up-regulated in SC-derived myoblasts treated with stromal derived factor-1 (SDF-1) and/or down-regulated in cells in which the expression of CXCR4 or CXCR7, that is, SDF-1 receptors, was silenced. SDF-1 is one of the important regulators of cell migration, mobilization, skeletal muscle regeneration, and angiogenesis. We hypothesized that selected miRNAs affect SC-derived myoblast fate and interactions with endothelial cells. We showed that miR-126a-3p inhibited both, myoblast migration and fusion. Moreover, the levels of Cxcl12, encoding SDF-1 and Ackr3, encoding CXCR7, were reduced by miR-126a-3p mimic. Interestingly, the miR-126a-3p mimic significantly decreased the level of numerous factors involved in myogenesis and the miR-126a-5p mimic increased the level of Vefga. Importantly, the treatment of endothelial cells with medium conditioned by miR-126-5p mimic transfected SC-derived myoblasts promoted tubulogenesis.https://doi.org/10.1038/s41598-023-41626-z |
| spellingShingle | Bartosz Mierzejewski Maria Anna Ciemerych Wladyslawa Streminska Katarzyna Janczyk-Ilach Edyta Brzoska miRNA-126a plays important role in myoblast and endothelial cell interaction Scientific Reports |
| title | miRNA-126a plays important role in myoblast and endothelial cell interaction |
| title_full | miRNA-126a plays important role in myoblast and endothelial cell interaction |
| title_fullStr | miRNA-126a plays important role in myoblast and endothelial cell interaction |
| title_full_unstemmed | miRNA-126a plays important role in myoblast and endothelial cell interaction |
| title_short | miRNA-126a plays important role in myoblast and endothelial cell interaction |
| title_sort | mirna 126a plays important role in myoblast and endothelial cell interaction |
| url | https://doi.org/10.1038/s41598-023-41626-z |
| work_keys_str_mv | AT bartoszmierzejewski mirna126aplaysimportantroleinmyoblastandendothelialcellinteraction AT mariaannaciemerych mirna126aplaysimportantroleinmyoblastandendothelialcellinteraction AT wladyslawastreminska mirna126aplaysimportantroleinmyoblastandendothelialcellinteraction AT katarzynajanczykilach mirna126aplaysimportantroleinmyoblastandendothelialcellinteraction AT edytabrzoska mirna126aplaysimportantroleinmyoblastandendothelialcellinteraction |