Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system

Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system

Mosaicism, including alleles comprising both wild-type and mutant, is a serious problem for gene modification by gene editing using electroporation. One-step generation of F0 pigs with completely desired gene modifications saves cost and time, but the major obstacles have been mosaic mutations. We h...

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Bibliographic Details
Main Authors: Zhao Namula, Manita Wittayarat, Lanh Thi Kim Do, Thanh Van Nguyen, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Takeshige Otoi
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:Veterinary and Animal Science
Subjects:
CRISPR/Cas9
Electroporation
Gene knockout
Permeability
Pig
Online Access:http://www.sciencedirect.com/science/article/pii/S2451943X22000126

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