Cisplatin-Impaired Adipogenic Differentiation of Adipose Mesenchymal Stem Cells
Adipose tissue-derived mesenchymal stem cells (ADSCs) are derived from adipose tissue and can be induced in vitro to differentiate into osteoblasts, chondroblasts, myocytes, neurons, and other cell types. Cisplatin is a commonly used chemotherapy drug for cancer patients. However, the effects of cis...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-06-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368917X694886 |
| _version_ | 1818328689785438208 |
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| author | Yu-Hsun Chang Hwan-Wun Liu Tang-Yuan Chu Yao-Tseng Wen Rong-Kung Tsai Dah-Ching Ding M.D., Ph.D. |
| author_facet | Yu-Hsun Chang Hwan-Wun Liu Tang-Yuan Chu Yao-Tseng Wen Rong-Kung Tsai Dah-Ching Ding M.D., Ph.D. |
| author_sort | Yu-Hsun Chang |
| collection | DOAJ |
| description | Adipose tissue-derived mesenchymal stem cells (ADSCs) are derived from adipose tissue and can be induced in vitro to differentiate into osteoblasts, chondroblasts, myocytes, neurons, and other cell types. Cisplatin is a commonly used chemotherapy drug for cancer patients. However, the effects of cisplatin on ADSCs remain elusive. This study found that a high concentration of cisplatin affects the viability of ADSCs. First, the IC 50 concentration of cisplatin was evaluated. Proliferation of ADSCs, as assessed by the XTT method, decreased immediately after treatment with various concentrations of cisplatin. ADSCs maintained mesenchymal stem cell surface markers after cisplatin treatment, as determined by flow cytometry. Upon differentiation by adding specific reagents, a significant decrease in adipogenic differentiation (by Oil red O staining) and osteogenic differentiation (by Alizarin red staining), and significant chondrogenic differentiation (by Alcian blue staining) were found after cisplatin treatment. Quantitative RT-PCR was also used in evaluating expression of specific genes to confirm differentiation. Finally, ADSCs from one donor who had received cisplatin showed significantly decreased adipogenic differentiation but increased osteogenic differentiation compared with ADSCs derived from one healthy donor. In conclusion, cisplatin affects the viability, proliferation, and differentiation of ADSCs both in vitro and in vivo via certain signaling pathways, such as p53 and Fas/FasL. The differentiation abilities of ADSCs should be evaluated before their transplantation for repairing cisplatin-induced tissue damage. |
| first_indexed | 2024-12-13T12:36:10Z |
| format | Article |
| id | doaj.art-dc7a228b42ec4b019af5f23ec26fab90 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-13T12:36:10Z |
| publishDate | 2017-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-dc7a228b42ec4b019af5f23ec26fab902022-12-21T23:45:52ZengSAGE PublishingCell Transplantation0963-68971555-38922017-06-012610.3727/096368917X694886Cisplatin-Impaired Adipogenic Differentiation of Adipose Mesenchymal Stem CellsYu-Hsun Chang0Hwan-Wun Liu1Tang-Yuan Chu2Yao-Tseng Wen3Rong-Kung Tsai4Dah-Ching Ding M.D., Ph.D.5Lab of Stem Cell Research, Department of Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, R.O.C.Department of Occupational Medicine, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, R.O.C.Department of Obstetrics and Gynecology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, R.O.C.Institute of Eye Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, R.O.C.Institute of Eye Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, R.O.C.Institue of Medical Science, Tzu Chi University, Hualien, Taiwan, R.O.C.Adipose tissue-derived mesenchymal stem cells (ADSCs) are derived from adipose tissue and can be induced in vitro to differentiate into osteoblasts, chondroblasts, myocytes, neurons, and other cell types. Cisplatin is a commonly used chemotherapy drug for cancer patients. However, the effects of cisplatin on ADSCs remain elusive. This study found that a high concentration of cisplatin affects the viability of ADSCs. First, the IC 50 concentration of cisplatin was evaluated. Proliferation of ADSCs, as assessed by the XTT method, decreased immediately after treatment with various concentrations of cisplatin. ADSCs maintained mesenchymal stem cell surface markers after cisplatin treatment, as determined by flow cytometry. Upon differentiation by adding specific reagents, a significant decrease in adipogenic differentiation (by Oil red O staining) and osteogenic differentiation (by Alizarin red staining), and significant chondrogenic differentiation (by Alcian blue staining) were found after cisplatin treatment. Quantitative RT-PCR was also used in evaluating expression of specific genes to confirm differentiation. Finally, ADSCs from one donor who had received cisplatin showed significantly decreased adipogenic differentiation but increased osteogenic differentiation compared with ADSCs derived from one healthy donor. In conclusion, cisplatin affects the viability, proliferation, and differentiation of ADSCs both in vitro and in vivo via certain signaling pathways, such as p53 and Fas/FasL. The differentiation abilities of ADSCs should be evaluated before their transplantation for repairing cisplatin-induced tissue damage.https://doi.org/10.3727/096368917X694886 |
| spellingShingle | Yu-Hsun Chang Hwan-Wun Liu Tang-Yuan Chu Yao-Tseng Wen Rong-Kung Tsai Dah-Ching Ding M.D., Ph.D. Cisplatin-Impaired Adipogenic Differentiation of Adipose Mesenchymal Stem Cells Cell Transplantation |
| title | Cisplatin-Impaired Adipogenic Differentiation of Adipose Mesenchymal Stem Cells |
| title_full | Cisplatin-Impaired Adipogenic Differentiation of Adipose Mesenchymal Stem Cells |
| title_fullStr | Cisplatin-Impaired Adipogenic Differentiation of Adipose Mesenchymal Stem Cells |
| title_full_unstemmed | Cisplatin-Impaired Adipogenic Differentiation of Adipose Mesenchymal Stem Cells |
| title_short | Cisplatin-Impaired Adipogenic Differentiation of Adipose Mesenchymal Stem Cells |
| title_sort | cisplatin impaired adipogenic differentiation of adipose mesenchymal stem cells |
| url | https://doi.org/10.3727/096368917X694886 |
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