Glutathione Fluorescence Sensing Based on a Co-Doped Carbon Dot/Manganese Dioxide Nanocoral Composite
Glutathione (GSH) is an antioxidant thiol that has a vital role in the pathogenesis of various human diseases such as cardiovascular disease and cancer. Hence, it is necessary to study effective methods of GSH evaluation. In our work, an effective GSH sensor based on a nitrogen and phosphorus co-dop...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/23/8677 |
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| author | Thi-Hoa Le Hyun-Jong Lee Quang-Nhat Tran |
| author_facet | Thi-Hoa Le Hyun-Jong Lee Quang-Nhat Tran |
| author_sort | Thi-Hoa Le |
| collection | DOAJ |
| description | Glutathione (GSH) is an antioxidant thiol that has a vital role in the pathogenesis of various human diseases such as cardiovascular disease and cancer. Hence, it is necessary to study effective methods of GSH evaluation. In our work, an effective GSH sensor based on a nitrogen and phosphorus co-doped carbon dot (NPCD)-MnO<sub>2</sub> nanocoral composite was fabricated. In addition to utilizing the strong fluorescence of the NPCDs, we utilized the reductant ability of the NPCDs themselves to form MnO<sub>2</sub> and then the NPCD-MnO<sub>2</sub> nanocoral composite from MnO<sub>4</sub><sup>−</sup>. The characteristics of the nanocoral composite were analyzed using various electron microscopy techniques and spectroscopic techniques. The overlap between the absorption spectrum of MnO<sub>2</sub> and the fluorescence emission spectrum of the NPCDs led to effective fluorescence resonance energy transfer (FRET) in the nanocoral composite, causing a decrease in the fluorescent intensity of the NPCDs. A linear recovery of the fluorescent intensity of the NPCDs was observed with the GSH level raising from 20 to 250 µM. Moreover, our GSH sensor showed high specificity and sensing potential in real samples with acceptable results. |
| first_indexed | 2024-03-09T17:42:03Z |
| format | Article |
| id | doaj.art-dc7bcb748fb84e48ad5613d2b7742e21 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T17:42:03Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-dc7bcb748fb84e48ad5613d2b7742e212023-11-24T11:32:17ZengMDPI AGMaterials1996-19442022-12-011523867710.3390/ma15238677Glutathione Fluorescence Sensing Based on a Co-Doped Carbon Dot/Manganese Dioxide Nanocoral CompositeThi-Hoa Le0Hyun-Jong Lee1Quang-Nhat Tran2Department of Chemical and Biological Engineering, Gachon University, Seongnam 13120, Republic of KoreaDepartment of Chemical and Biological Engineering, Gachon University, Seongnam 13120, Republic of KoreaDepartment of Chemical and Biological Engineering, Gachon University, Seongnam 13120, Republic of KoreaGlutathione (GSH) is an antioxidant thiol that has a vital role in the pathogenesis of various human diseases such as cardiovascular disease and cancer. Hence, it is necessary to study effective methods of GSH evaluation. In our work, an effective GSH sensor based on a nitrogen and phosphorus co-doped carbon dot (NPCD)-MnO<sub>2</sub> nanocoral composite was fabricated. In addition to utilizing the strong fluorescence of the NPCDs, we utilized the reductant ability of the NPCDs themselves to form MnO<sub>2</sub> and then the NPCD-MnO<sub>2</sub> nanocoral composite from MnO<sub>4</sub><sup>−</sup>. The characteristics of the nanocoral composite were analyzed using various electron microscopy techniques and spectroscopic techniques. The overlap between the absorption spectrum of MnO<sub>2</sub> and the fluorescence emission spectrum of the NPCDs led to effective fluorescence resonance energy transfer (FRET) in the nanocoral composite, causing a decrease in the fluorescent intensity of the NPCDs. A linear recovery of the fluorescent intensity of the NPCDs was observed with the GSH level raising from 20 to 250 µM. Moreover, our GSH sensor showed high specificity and sensing potential in real samples with acceptable results.https://www.mdpi.com/1996-1944/15/23/8677GlutathioneNPCD-MnO<sub>2</sub> nanocoral compositefluorescence resonance energy transfer (FRET)“turn on” fluorescence |
| spellingShingle | Thi-Hoa Le Hyun-Jong Lee Quang-Nhat Tran Glutathione Fluorescence Sensing Based on a Co-Doped Carbon Dot/Manganese Dioxide Nanocoral Composite Materials Glutathione NPCD-MnO<sub>2</sub> nanocoral composite fluorescence resonance energy transfer (FRET) “turn on” fluorescence |
| title | Glutathione Fluorescence Sensing Based on a Co-Doped Carbon Dot/Manganese Dioxide Nanocoral Composite |
| title_full | Glutathione Fluorescence Sensing Based on a Co-Doped Carbon Dot/Manganese Dioxide Nanocoral Composite |
| title_fullStr | Glutathione Fluorescence Sensing Based on a Co-Doped Carbon Dot/Manganese Dioxide Nanocoral Composite |
| title_full_unstemmed | Glutathione Fluorescence Sensing Based on a Co-Doped Carbon Dot/Manganese Dioxide Nanocoral Composite |
| title_short | Glutathione Fluorescence Sensing Based on a Co-Doped Carbon Dot/Manganese Dioxide Nanocoral Composite |
| title_sort | glutathione fluorescence sensing based on a co doped carbon dot manganese dioxide nanocoral composite |
| topic | Glutathione NPCD-MnO<sub>2</sub> nanocoral composite fluorescence resonance energy transfer (FRET) “turn on” fluorescence |
| url | https://www.mdpi.com/1996-1944/15/23/8677 |
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