In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea
The aim of the current research was to find the best plant growth regulators for the multiplication of Sarracenia purpurea. Murashige and Skoog medium (MS) was prepared with macronutrients and micronutrients at 1/3 strength, full strength vitamins, supplemented with 30 g/l sucrose and 5 g/l phytagel...
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| Format: | Article |
| Language: | English |
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AcademicPres
2018-12-01
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| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | http://journals.usamvcluj.ro/index.php/zootehnie/article/view/13164 |
| _version_ | 1819088152408621056 |
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| author | Ileana MICLEA Rita BERNAT |
| author_facet | Ileana MICLEA Rita BERNAT |
| author_sort | Ileana MICLEA |
| collection | DOAJ |
| description | The aim of the current research was to find the best plant growth regulators for the multiplication of Sarracenia purpurea. Murashige and Skoog medium (MS) was prepared with macronutrients and micronutrients at 1/3 strength, full strength vitamins, supplemented with 30 g/l sucrose and 5 g/l phytagel and autoclaved. After cooling 0.5 mg\l α-naphthaleneacetic acid (NAA), 5 mg\l 6-benzyladenine (BA) or 0.5 mg\l NAA + 3 mg\l BA were added. Young S. purpurea plants were selected and transferred to media with or without plant growth regulators and cultured for 12 weeks. At the end of this time frame number of roots, root length (cm) and number of shoots were evaluated and differences were analysed by the analysis of variance and interpreted using the Tuckey test. The largest number of roots grew in medium supplemented with 0.5 mg\l NAA but the the absence of plant growth regulators increased their length. The best conditions for shoot multiplication were provided by supplementing 1/3MS with 5 mg\l BA. |
| first_indexed | 2024-12-21T21:47:30Z |
| format | Article |
| id | doaj.art-dc7c573867234d63b4fa2ce9df168740 |
| institution | Directory Open Access Journal |
| issn | 1843-5262 1843-536X |
| language | English |
| last_indexed | 2024-12-21T21:47:30Z |
| publishDate | 2018-12-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies |
| spelling | doaj.art-dc7c573867234d63b4fa2ce9df1687402022-12-21T18:49:11ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies1843-52621843-536X2018-12-0175213413610.15835/buasvmcn-asb:2018.001810779In vitro Multiplication of the Pitcher Plant Sarracenia PurpureaIleana MICLEA0Rita BERNAT1University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5, Mănăştur Street, 400372University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5, Mănăştur Street, 400372The aim of the current research was to find the best plant growth regulators for the multiplication of Sarracenia purpurea. Murashige and Skoog medium (MS) was prepared with macronutrients and micronutrients at 1/3 strength, full strength vitamins, supplemented with 30 g/l sucrose and 5 g/l phytagel and autoclaved. After cooling 0.5 mg\l α-naphthaleneacetic acid (NAA), 5 mg\l 6-benzyladenine (BA) or 0.5 mg\l NAA + 3 mg\l BA were added. Young S. purpurea plants were selected and transferred to media with or without plant growth regulators and cultured for 12 weeks. At the end of this time frame number of roots, root length (cm) and number of shoots were evaluated and differences were analysed by the analysis of variance and interpreted using the Tuckey test. The largest number of roots grew in medium supplemented with 0.5 mg\l NAA but the the absence of plant growth regulators increased their length. The best conditions for shoot multiplication were provided by supplementing 1/3MS with 5 mg\l BA.http://journals.usamvcluj.ro/index.php/zootehnie/article/view/13164in vitro propagationSarraccenia purpurea. |
| spellingShingle | Ileana MICLEA Rita BERNAT In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies in vitro propagation Sarraccenia purpurea. |
| title | In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea |
| title_full | In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea |
| title_fullStr | In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea |
| title_full_unstemmed | In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea |
| title_short | In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea |
| title_sort | in vitro multiplication of the pitcher plant sarracenia purpurea |
| topic | in vitro propagation Sarraccenia purpurea. |
| url | http://journals.usamvcluj.ro/index.php/zootehnie/article/view/13164 |
| work_keys_str_mv | AT ileanamiclea invitromultiplicationofthepitcherplantsarraceniapurpurea AT ritabernat invitromultiplicationofthepitcherplantsarraceniapurpurea |