Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2
The human embryonic stem cell line NERCe002-A-2 was generated by transduction of NERCe002-A cells with an expression vector carrying the luciferase gene. The stem cells labelled with luciferase can be transplanted into animals and detected by the bioluminescence imaging technology. This provides opt...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-04-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506118300540 |
| _version_ | 1819151775801802752 |
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| author | Yingying Peng Menghan Xie Xingxiang Duan Liang Hu Juan Yu Sicong Zeng Yang Wang Qi Ouyang Guangxiu Lu Ge Lin Yi Sun |
| author_facet | Yingying Peng Menghan Xie Xingxiang Duan Liang Hu Juan Yu Sicong Zeng Yang Wang Qi Ouyang Guangxiu Lu Ge Lin Yi Sun |
| author_sort | Yingying Peng |
| collection | DOAJ |
| description | The human embryonic stem cell line NERCe002-A-2 was generated by transduction of NERCe002-A cells with an expression vector carrying the luciferase gene. The stem cells labelled with luciferase can be transplanted into animals and detected by the bioluminescence imaging technology. This provides optimal prospects of application to in vivo stem cell tracing. Luciferin served as a substrate to detect the activity of luciferase, and luciferase expression was measured by quantitative PCR. Characterization assays suggested that the NERCe002-A-2 cell line expresses typical markers of pluripotency and can form the 3 germ layers in vivo. |
| first_indexed | 2024-12-22T14:38:46Z |
| format | Article |
| id | doaj.art-dc7d5dff4d78436f83f41bff41062579 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-22T14:38:46Z |
| publishDate | 2018-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-dc7d5dff4d78436f83f41bff410625792022-12-21T18:22:36ZengElsevierStem Cell Research1873-50612018-04-0128172176Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2Yingying Peng0Menghan Xie1Xingxiang Duan2Liang Hu3Juan Yu4Sicong Zeng5Yang Wang6Qi Ouyang7Guangxiu Lu8Ge Lin9Yi Sun10Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, ChinaNational Engineering and Research Center of Human Stem Cells, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, ChinaNational Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Corresponding author at: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.The human embryonic stem cell line NERCe002-A-2 was generated by transduction of NERCe002-A cells with an expression vector carrying the luciferase gene. The stem cells labelled with luciferase can be transplanted into animals and detected by the bioluminescence imaging technology. This provides optimal prospects of application to in vivo stem cell tracing. Luciferin served as a substrate to detect the activity of luciferase, and luciferase expression was measured by quantitative PCR. Characterization assays suggested that the NERCe002-A-2 cell line expresses typical markers of pluripotency and can form the 3 germ layers in vivo.http://www.sciencedirect.com/science/article/pii/S1873506118300540 |
| spellingShingle | Yingying Peng Menghan Xie Xingxiang Duan Liang Hu Juan Yu Sicong Zeng Yang Wang Qi Ouyang Guangxiu Lu Ge Lin Yi Sun Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2 Stem Cell Research |
| title | Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2 |
| title_full | Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2 |
| title_fullStr | Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2 |
| title_full_unstemmed | Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2 |
| title_short | Generation of a luciferase-expressing human embryonic stem cell line: NERCe002-A-2 |
| title_sort | generation of a luciferase expressing human embryonic stem cell line nerce002 a 2 |
| url | http://www.sciencedirect.com/science/article/pii/S1873506118300540 |
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