Assessment of the Hypoglycemic and Hypolipidemic Activity of Flavonoid-Rich Extract from <i>Angelica keiskei</i>
<i>Angelica keiskei</i> contains a variety of bioactive compounds including chalcone, coumarin, and phytochemicals, endowing it with pharmacological effects such as lipid-lowering activity, antitumor activity, liver protection, and nerve protection. This study aims to study the hypoglyce...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/19/6625 |
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| author | Lanlan Tu Rui Wang Zheng Fang Mengge Sun Xiaohui Sun Jinhong Wu Yali Dang Jianhua Liu |
| author_facet | Lanlan Tu Rui Wang Zheng Fang Mengge Sun Xiaohui Sun Jinhong Wu Yali Dang Jianhua Liu |
| author_sort | Lanlan Tu |
| collection | DOAJ |
| description | <i>Angelica keiskei</i> contains a variety of bioactive compounds including chalcone, coumarin, and phytochemicals, endowing it with pharmacological effects such as lipid-lowering activity, antitumor activity, liver protection, and nerve protection. This study aims to study the hypoglycemic and hypolipidemic effects of the flavonoid-rich extract from <i>Angelica keiskei</i> (FEAK) in an effort to exploit new applications of FEAK and increase its commercial value. In this paper, flavonoid compounds in <i>Angelica keiskei</i> were extracted using 50% ethanol, and the contents of the flavonoid compounds were analyzed by UPLC-MS/MS. Then, the hypoglycemic and hypolipidemic activities of the FEAK were investigated through in vitro enzyme activity and cell experiments as well as establishing in vivo zebrafish and Caenorhabditis elegans (<i>C. elegans</i>) models. The UPLC-MS/MS results show that the major flavonoid compounds in the FEAK were aureusidin, xanthoangelol, kaempferol, luteolin, and quercetin. The inhibitory rates of the FEAK on the activity of α-amylase and cholesterol esterase were 57.13% and 72.11%, respectively. In cell lipid-lowering experiments, the FEAK significantly reduced the total cholesterol (TC) and total triglyceride (TG) levels in a dose-dependent manner, with 150 μg/mL of FEAK decreasing the intracellular levels of TC and TG by 33.86% and 27.89%, respectively. The fluorescence intensity of the FEAK group was 68.12% higher than that of the control group, indicating that the FEAK exhibited hypoglycemic effects. When the concentration of the FEAK reached 500 μg/mL, the hypoglycemic effect on zebrafish reached up to 57.7%, and the average fluorescence intensity of <i>C. elegans</i> in the FEAK group was 17% lower than that of the control group. The results indicate that the FEAK had hypoglycemic and hypolipidemic activities. The findings of this study provide theoretical references for the high-value utilization of <i>Angelica keiskei</i> and the development of natural functional food with hypoglycemic and hypolipidemic activities. |
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| language | English |
| last_indexed | 2024-03-09T21:24:08Z |
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| spelling | doaj.art-dc7fe132a29745e489628a866cadd6232023-11-23T21:14:32ZengMDPI AGMolecules1420-30492022-10-012719662510.3390/molecules27196625Assessment of the Hypoglycemic and Hypolipidemic Activity of Flavonoid-Rich Extract from <i>Angelica keiskei</i>Lanlan Tu0Rui Wang1Zheng Fang2Mengge Sun3Xiaohui Sun4Jinhong Wu5Yali Dang6Jianhua Liu7Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, ChinaDepartment of Laboratory Medicine, School of Medicine, Institute of Molecular Medicine, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, ChinaInternational Faculty of Applied Technology, Yibin University, Yibin 644000, China<i>Angelica keiskei</i> contains a variety of bioactive compounds including chalcone, coumarin, and phytochemicals, endowing it with pharmacological effects such as lipid-lowering activity, antitumor activity, liver protection, and nerve protection. This study aims to study the hypoglycemic and hypolipidemic effects of the flavonoid-rich extract from <i>Angelica keiskei</i> (FEAK) in an effort to exploit new applications of FEAK and increase its commercial value. In this paper, flavonoid compounds in <i>Angelica keiskei</i> were extracted using 50% ethanol, and the contents of the flavonoid compounds were analyzed by UPLC-MS/MS. Then, the hypoglycemic and hypolipidemic activities of the FEAK were investigated through in vitro enzyme activity and cell experiments as well as establishing in vivo zebrafish and Caenorhabditis elegans (<i>C. elegans</i>) models. The UPLC-MS/MS results show that the major flavonoid compounds in the FEAK were aureusidin, xanthoangelol, kaempferol, luteolin, and quercetin. The inhibitory rates of the FEAK on the activity of α-amylase and cholesterol esterase were 57.13% and 72.11%, respectively. In cell lipid-lowering experiments, the FEAK significantly reduced the total cholesterol (TC) and total triglyceride (TG) levels in a dose-dependent manner, with 150 μg/mL of FEAK decreasing the intracellular levels of TC and TG by 33.86% and 27.89%, respectively. The fluorescence intensity of the FEAK group was 68.12% higher than that of the control group, indicating that the FEAK exhibited hypoglycemic effects. When the concentration of the FEAK reached 500 μg/mL, the hypoglycemic effect on zebrafish reached up to 57.7%, and the average fluorescence intensity of <i>C. elegans</i> in the FEAK group was 17% lower than that of the control group. The results indicate that the FEAK had hypoglycemic and hypolipidemic activities. The findings of this study provide theoretical references for the high-value utilization of <i>Angelica keiskei</i> and the development of natural functional food with hypoglycemic and hypolipidemic activities.https://www.mdpi.com/1420-3049/27/19/6625hypoglycemic activityhypolipidemic activity<i>Angelica keiskei</i>flavonoidfunction evaluation |
| spellingShingle | Lanlan Tu Rui Wang Zheng Fang Mengge Sun Xiaohui Sun Jinhong Wu Yali Dang Jianhua Liu Assessment of the Hypoglycemic and Hypolipidemic Activity of Flavonoid-Rich Extract from <i>Angelica keiskei</i> Molecules hypoglycemic activity hypolipidemic activity <i>Angelica keiskei</i> flavonoid function evaluation |
| title | Assessment of the Hypoglycemic and Hypolipidemic Activity of Flavonoid-Rich Extract from <i>Angelica keiskei</i> |
| title_full | Assessment of the Hypoglycemic and Hypolipidemic Activity of Flavonoid-Rich Extract from <i>Angelica keiskei</i> |
| title_fullStr | Assessment of the Hypoglycemic and Hypolipidemic Activity of Flavonoid-Rich Extract from <i>Angelica keiskei</i> |
| title_full_unstemmed | Assessment of the Hypoglycemic and Hypolipidemic Activity of Flavonoid-Rich Extract from <i>Angelica keiskei</i> |
| title_short | Assessment of the Hypoglycemic and Hypolipidemic Activity of Flavonoid-Rich Extract from <i>Angelica keiskei</i> |
| title_sort | assessment of the hypoglycemic and hypolipidemic activity of flavonoid rich extract from i angelica keiskei i |
| topic | hypoglycemic activity hypolipidemic activity <i>Angelica keiskei</i> flavonoid function evaluation |
| url | https://www.mdpi.com/1420-3049/27/19/6625 |
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