Prevalence and characterization of beta-lactamase-producing Escherichia coli isolates from a tertiary care hospital in India
BACKGROUND: The purpose of the study was to determine the prevalence and characterize the resistance profiles of Escherichia coli isolated from various clinical specimens by various phenotypic and genotypic methods. MATERIALS AND METHODS: A total of 196 consecutive, nonduplicate strains o...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Thieme Medical and Scientific Publishers Pvt. Ltd.
2019-04-01
|
| Series: | Journal of Laboratory Physicians |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_122_18 |
| _version_ | 1818173745260396544 |
|---|---|
| author | Aishwarya Govindaswamy Vijeta Bajpai Surbhi Khurana Anjana Aravinda Priyam Batra Rajesh Malhotra Purva Mathur |
| author_facet | Aishwarya Govindaswamy Vijeta Bajpai Surbhi Khurana Anjana Aravinda Priyam Batra Rajesh Malhotra Purva Mathur |
| author_sort | Aishwarya Govindaswamy |
| collection | DOAJ |
| description | BACKGROUND: The purpose of the study was to determine the prevalence and characterize the resistance profiles of Escherichia coli isolated from various clinical specimens by various phenotypic and genotypic methods.
MATERIALS AND METHODS: A total of 196 consecutive, nonduplicate strains of clinically significant E. coli isolated from various clinical specimens were included in the study. Identification and antimicrobial susceptibility testing was performed by using Vitek-2 system (Biomerieux, France). Phenotypic detection of extended spectrum beta-lactamase (ESBLs), Amp-C-β lactamase (Amp C), and carbapenemase production was done by various combination of disc diffusion methods, minimum inhibitory concentration determination by E-test, followed by polymerase-chain-reaction for the detection of β-lactamase-encoding genes.
RESULTS: Overall prevalence of ESBLs, Amp C, and carbapenemase production was found to be 88.3%, 42.2%, and 65.1% by the phenotypic detection methods. Our study also revealed high resistance rates against other antibiotics such as cefepime (89%), cefotaxime (95.4%), ceftazidime (85.4%), ceftriaxone (91.8%), cefpodoxime (92.7%), aztreonam (56.3%), piperacillin/tazobactam (89.2%), and ticarcillin/clavulanic acid (76.3%). The most prevalent ESBL gene was blaTEM(67.30%), and least prevalent ESBL gene was blaVEB(2.61%). In case of Amp C, blaFOXgene (21.9%) was predominant. Among the genes encoding for carbapenemases, the most common gene was blaNDM(61.7%) followed by blaVIM(30.8%), blaKPC(10.6%), blaOXA-48 (5.3%), and blaIMP(2.1%).
CONCLUSION: Our findings suggest a high rate of ESBLs, Amp C, and carbapenemase production among the E. coli isolates. A combination of both phenotypic and genotypic methods would be ideal for better characterization of resistance patterns among the E. coli isolates. |
| first_indexed | 2024-12-11T19:33:23Z |
| format | Article |
| id | doaj.art-dc837a87037b40ef9edad8fd90a6de13 |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-11T19:33:23Z |
| publishDate | 2019-04-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-dc837a87037b40ef9edad8fd90a6de132022-12-22T00:53:13ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262019-04-01110212312710.4103/JLP.JLP_122_18Prevalence and characterization of beta-lactamase-producing Escherichia coli isolates from a tertiary care hospital in IndiaAishwarya Govindaswamy0Vijeta Bajpai1Surbhi Khurana2Anjana Aravinda3Priyam Batra4Rajesh Malhotra5Purva Mathur6Department of Microbiology, Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi, IndiaDepartment of Microbiology, Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi, IndiaDepartment of Microbiology, Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi, IndiaDepartment of Microbiology, Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi, IndiaDepartment of Microbiology, Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi, IndiaDepartment of Orthopedics, All India Institute of Medical Sciences, New Delhi, IndiaDepartment of Microbiology, Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi, IndiaBACKGROUND: The purpose of the study was to determine the prevalence and characterize the resistance profiles of Escherichia coli isolated from various clinical specimens by various phenotypic and genotypic methods. MATERIALS AND METHODS: A total of 196 consecutive, nonduplicate strains of clinically significant E. coli isolated from various clinical specimens were included in the study. Identification and antimicrobial susceptibility testing was performed by using Vitek-2 system (Biomerieux, France). Phenotypic detection of extended spectrum beta-lactamase (ESBLs), Amp-C-β lactamase (Amp C), and carbapenemase production was done by various combination of disc diffusion methods, minimum inhibitory concentration determination by E-test, followed by polymerase-chain-reaction for the detection of β-lactamase-encoding genes. RESULTS: Overall prevalence of ESBLs, Amp C, and carbapenemase production was found to be 88.3%, 42.2%, and 65.1% by the phenotypic detection methods. Our study also revealed high resistance rates against other antibiotics such as cefepime (89%), cefotaxime (95.4%), ceftazidime (85.4%), ceftriaxone (91.8%), cefpodoxime (92.7%), aztreonam (56.3%), piperacillin/tazobactam (89.2%), and ticarcillin/clavulanic acid (76.3%). The most prevalent ESBL gene was blaTEM(67.30%), and least prevalent ESBL gene was blaVEB(2.61%). In case of Amp C, blaFOXgene (21.9%) was predominant. Among the genes encoding for carbapenemases, the most common gene was blaNDM(61.7%) followed by blaVIM(30.8%), blaKPC(10.6%), blaOXA-48 (5.3%), and blaIMP(2.1%). CONCLUSION: Our findings suggest a high rate of ESBLs, Amp C, and carbapenemase production among the E. coli isolates. A combination of both phenotypic and genotypic methods would be ideal for better characterization of resistance patterns among the E. coli isolates.http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_122_18amp-c-β lactamasecarbapenemases escherichia coli extended spectrum beta-lactamases |
| spellingShingle | Aishwarya Govindaswamy Vijeta Bajpai Surbhi Khurana Anjana Aravinda Priyam Batra Rajesh Malhotra Purva Mathur Prevalence and characterization of beta-lactamase-producing Escherichia coli isolates from a tertiary care hospital in India Journal of Laboratory Physicians amp-c-β lactamase carbapenemases escherichia coli extended spectrum beta-lactamases |
| title | Prevalence and characterization of beta-lactamase-producing Escherichia coli isolates from a tertiary care hospital in India |
| title_full | Prevalence and characterization of beta-lactamase-producing Escherichia coli isolates from a tertiary care hospital in India |
| title_fullStr | Prevalence and characterization of beta-lactamase-producing Escherichia coli isolates from a tertiary care hospital in India |
| title_full_unstemmed | Prevalence and characterization of beta-lactamase-producing Escherichia coli isolates from a tertiary care hospital in India |
| title_short | Prevalence and characterization of beta-lactamase-producing Escherichia coli isolates from a tertiary care hospital in India |
| title_sort | prevalence and characterization of beta lactamase producing escherichia coli isolates from a tertiary care hospital in india |
| topic | amp-c-β lactamase carbapenemases escherichia coli extended spectrum beta-lactamases |
| url | http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_122_18 |
| work_keys_str_mv | AT aishwaryagovindaswamy prevalenceandcharacterizationofbetalactamaseproducingescherichiacoliisolatesfromatertiarycarehospitalinindia AT vijetabajpai prevalenceandcharacterizationofbetalactamaseproducingescherichiacoliisolatesfromatertiarycarehospitalinindia AT surbhikhurana prevalenceandcharacterizationofbetalactamaseproducingescherichiacoliisolatesfromatertiarycarehospitalinindia AT anjanaaravinda prevalenceandcharacterizationofbetalactamaseproducingescherichiacoliisolatesfromatertiarycarehospitalinindia AT priyambatra prevalenceandcharacterizationofbetalactamaseproducingescherichiacoliisolatesfromatertiarycarehospitalinindia AT rajeshmalhotra prevalenceandcharacterizationofbetalactamaseproducingescherichiacoliisolatesfromatertiarycarehospitalinindia AT purvamathur prevalenceandcharacterizationofbetalactamaseproducingescherichiacoliisolatesfromatertiarycarehospitalinindia |