Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure

The study examined the potential of Silymarin, a blend of bioactive flavonolignans extracted from the milk thistle Silybum marianum, to mitigate Deltamethrin-induced toxicity in the blood of Cyprinus carpio. Fish were exposed to Deltamethrin (0.66 μg/L), the plant extract, or a combination of both f...

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Main Authors: Rajinder Jindal, Ritu Sharma, Parminder Kaur, Sukhmani Kaur, Cristiana Roberta Multisanti, Caterina Faggio
Format: Article
Language:English
Published: Elsevier 2024-04-01
Series:Heliyon
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024044505
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author Rajinder Jindal
Ritu Sharma
Parminder Kaur
Sukhmani Kaur
Cristiana Roberta Multisanti
Caterina Faggio
author_facet Rajinder Jindal
Ritu Sharma
Parminder Kaur
Sukhmani Kaur
Cristiana Roberta Multisanti
Caterina Faggio
author_sort Rajinder Jindal
collection DOAJ
description The study examined the potential of Silymarin, a blend of bioactive flavonolignans extracted from the milk thistle Silybum marianum, to mitigate Deltamethrin-induced toxicity in the blood of Cyprinus carpio. Fish were exposed to Deltamethrin (0.66 μg/L), the plant extract, or a combination of both for a duration of thirty days. Various parameters, including serum biochemical markers, erythrocytic abnormalities, and genotoxicity endpoints, were assessed. Results indicated a significant (p < 0.05) increase in the levels of AST, ALT, ALP, blood urea nitrogen, creatinine, glucose, cholesterol, and TLC in the fish exposed to the pesticide. Conversely, total protein, TEC, and Hb showed a notable decrease. There was also a notable rise in micronuclei and erythrocytic abnormalities such as acanthocytes, microcytes, and notched cells. Under ultrastructural examination, phenotypic deformities like spherocytosis, discocytes, and clumped erythrocytes were observed. However, dietary supplementation of silymarin (1 g/kg) significantly restored the biochemical, genetic, and cellular parameters, resembling those of the control group. This suggests the potential of this plant extract in protecting the common carp, Cyprinus carpio, from Deltamethrin-induced damage by scavenging free radicals and reducing DNA oxidative stress.
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spelling doaj.art-dc840822cf37404588e4efa5d620f24c2024-03-29T05:50:39ZengElsevierHeliyon2405-84402024-04-01107e28419Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposureRajinder Jindal0Ritu Sharma1Parminder Kaur2Sukhmani Kaur3Cristiana Roberta Multisanti4Caterina Faggio5Aquatic Biology Laboratory, Department of Zoology, Panjab University, Chandigarh, 160014, IndiaDepartment of Hepatology, Postgraduate Institute of Medical Education and Research, Chandigarh 160014, IndiaDepartment of Biosciences, University Institute of Biotechnology, Chandigarh University, Punjab, IndiaDepartment of Environment Studies, Panjab University, Chandigarh, 160014, IndiaDepartment of Veterinary Sciences, University of Messina, Viale Giovanni Palatucci snc, 98168 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences University of Messina-Italy Messina, Italy; Department of Eco-sustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Naples, Italy; Corresponding author. Department of Chemical, Biological, Pharmaceutical and Environmental Sciences University of Messina-Italy Messina, Italy.The study examined the potential of Silymarin, a blend of bioactive flavonolignans extracted from the milk thistle Silybum marianum, to mitigate Deltamethrin-induced toxicity in the blood of Cyprinus carpio. Fish were exposed to Deltamethrin (0.66 μg/L), the plant extract, or a combination of both for a duration of thirty days. Various parameters, including serum biochemical markers, erythrocytic abnormalities, and genotoxicity endpoints, were assessed. Results indicated a significant (p < 0.05) increase in the levels of AST, ALT, ALP, blood urea nitrogen, creatinine, glucose, cholesterol, and TLC in the fish exposed to the pesticide. Conversely, total protein, TEC, and Hb showed a notable decrease. There was also a notable rise in micronuclei and erythrocytic abnormalities such as acanthocytes, microcytes, and notched cells. Under ultrastructural examination, phenotypic deformities like spherocytosis, discocytes, and clumped erythrocytes were observed. However, dietary supplementation of silymarin (1 g/kg) significantly restored the biochemical, genetic, and cellular parameters, resembling those of the control group. This suggests the potential of this plant extract in protecting the common carp, Cyprinus carpio, from Deltamethrin-induced damage by scavenging free radicals and reducing DNA oxidative stress.http://www.sciencedirect.com/science/article/pii/S2405844024044505DeltamethrinSilymarinCyprinus carpioHaematologyCytotoxicity
spellingShingle Rajinder Jindal
Ritu Sharma
Parminder Kaur
Sukhmani Kaur
Cristiana Roberta Multisanti
Caterina Faggio
Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure
Heliyon
Deltamethrin
Silymarin
Cyprinus carpio
Haematology
Cytotoxicity
title Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure
title_full Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure
title_fullStr Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure
title_full_unstemmed Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure
title_short Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure
title_sort mitigation of haemato genotoxic and stress response effects in cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure
topic Deltamethrin
Silymarin
Cyprinus carpio
Haematology
Cytotoxicity
url http://www.sciencedirect.com/science/article/pii/S2405844024044505
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