Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure
The study examined the potential of Silymarin, a blend of bioactive flavonolignans extracted from the milk thistle Silybum marianum, to mitigate Deltamethrin-induced toxicity in the blood of Cyprinus carpio. Fish were exposed to Deltamethrin (0.66 μg/L), the plant extract, or a combination of both f...
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Elsevier
2024-04-01
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author | Rajinder Jindal Ritu Sharma Parminder Kaur Sukhmani Kaur Cristiana Roberta Multisanti Caterina Faggio |
author_facet | Rajinder Jindal Ritu Sharma Parminder Kaur Sukhmani Kaur Cristiana Roberta Multisanti Caterina Faggio |
author_sort | Rajinder Jindal |
collection | DOAJ |
description | The study examined the potential of Silymarin, a blend of bioactive flavonolignans extracted from the milk thistle Silybum marianum, to mitigate Deltamethrin-induced toxicity in the blood of Cyprinus carpio. Fish were exposed to Deltamethrin (0.66 μg/L), the plant extract, or a combination of both for a duration of thirty days. Various parameters, including serum biochemical markers, erythrocytic abnormalities, and genotoxicity endpoints, were assessed. Results indicated a significant (p < 0.05) increase in the levels of AST, ALT, ALP, blood urea nitrogen, creatinine, glucose, cholesterol, and TLC in the fish exposed to the pesticide. Conversely, total protein, TEC, and Hb showed a notable decrease. There was also a notable rise in micronuclei and erythrocytic abnormalities such as acanthocytes, microcytes, and notched cells. Under ultrastructural examination, phenotypic deformities like spherocytosis, discocytes, and clumped erythrocytes were observed. However, dietary supplementation of silymarin (1 g/kg) significantly restored the biochemical, genetic, and cellular parameters, resembling those of the control group. This suggests the potential of this plant extract in protecting the common carp, Cyprinus carpio, from Deltamethrin-induced damage by scavenging free radicals and reducing DNA oxidative stress. |
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issn | 2405-8440 |
language | English |
last_indexed | 2024-04-24T16:50:02Z |
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spelling | doaj.art-dc840822cf37404588e4efa5d620f24c2024-03-29T05:50:39ZengElsevierHeliyon2405-84402024-04-01107e28419Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposureRajinder Jindal0Ritu Sharma1Parminder Kaur2Sukhmani Kaur3Cristiana Roberta Multisanti4Caterina Faggio5Aquatic Biology Laboratory, Department of Zoology, Panjab University, Chandigarh, 160014, IndiaDepartment of Hepatology, Postgraduate Institute of Medical Education and Research, Chandigarh 160014, IndiaDepartment of Biosciences, University Institute of Biotechnology, Chandigarh University, Punjab, IndiaDepartment of Environment Studies, Panjab University, Chandigarh, 160014, IndiaDepartment of Veterinary Sciences, University of Messina, Viale Giovanni Palatucci snc, 98168 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences University of Messina-Italy Messina, Italy; Department of Eco-sustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Naples, Italy; Corresponding author. Department of Chemical, Biological, Pharmaceutical and Environmental Sciences University of Messina-Italy Messina, Italy.The study examined the potential of Silymarin, a blend of bioactive flavonolignans extracted from the milk thistle Silybum marianum, to mitigate Deltamethrin-induced toxicity in the blood of Cyprinus carpio. Fish were exposed to Deltamethrin (0.66 μg/L), the plant extract, or a combination of both for a duration of thirty days. Various parameters, including serum biochemical markers, erythrocytic abnormalities, and genotoxicity endpoints, were assessed. Results indicated a significant (p < 0.05) increase in the levels of AST, ALT, ALP, blood urea nitrogen, creatinine, glucose, cholesterol, and TLC in the fish exposed to the pesticide. Conversely, total protein, TEC, and Hb showed a notable decrease. There was also a notable rise in micronuclei and erythrocytic abnormalities such as acanthocytes, microcytes, and notched cells. Under ultrastructural examination, phenotypic deformities like spherocytosis, discocytes, and clumped erythrocytes were observed. However, dietary supplementation of silymarin (1 g/kg) significantly restored the biochemical, genetic, and cellular parameters, resembling those of the control group. This suggests the potential of this plant extract in protecting the common carp, Cyprinus carpio, from Deltamethrin-induced damage by scavenging free radicals and reducing DNA oxidative stress.http://www.sciencedirect.com/science/article/pii/S2405844024044505DeltamethrinSilymarinCyprinus carpioHaematologyCytotoxicity |
spellingShingle | Rajinder Jindal Ritu Sharma Parminder Kaur Sukhmani Kaur Cristiana Roberta Multisanti Caterina Faggio Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure Heliyon Deltamethrin Silymarin Cyprinus carpio Haematology Cytotoxicity |
title | Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure |
title_full | Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure |
title_fullStr | Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure |
title_full_unstemmed | Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure |
title_short | Mitigation of haemato-genotoxic and stress response effects in Cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure |
title_sort | mitigation of haemato genotoxic and stress response effects in cyprinus carpio via silymarin dietary supplementation following deltamethrin exposure |
topic | Deltamethrin Silymarin Cyprinus carpio Haematology Cytotoxicity |
url | http://www.sciencedirect.com/science/article/pii/S2405844024044505 |
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