Aquaporin-11 (AQP11) Expression in the Mouse Brain
Aquaporin-11 (AQP11) is an intracellular aquaporin expressed in various tissues, including brain tissues in mammals. While AQP11-deficient mice have developed fatal polycystic kidneys at one month old, the role of AQP11 in the brain was not well appreciated. In this study, we examined the AQP11 expr...
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MDPI AG
2016-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/17/6/861 |
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| author | Shin Koike Yasuko Tanaka Toshiyuki Matsuzaki Yoshiyuki Morishita Kenichi Ishibashi |
| author_facet | Shin Koike Yasuko Tanaka Toshiyuki Matsuzaki Yoshiyuki Morishita Kenichi Ishibashi |
| author_sort | Shin Koike |
| collection | DOAJ |
| description | Aquaporin-11 (AQP11) is an intracellular aquaporin expressed in various tissues, including brain tissues in mammals. While AQP11-deficient mice have developed fatal polycystic kidneys at one month old, the role of AQP11 in the brain was not well appreciated. In this study, we examined the AQP11 expression in the mouse brain and the brain phenotype of AQP11-deficient mice. AQP11 messenger ribonucleic acid (mRNA) and protein were expressed in the brain, but much less than in the thymus and kidney. Immunostaining showed that AQP11 was localized at the epithelium of the choroid plexus and at the endothelium of the brain capillary, suggesting that AQP11 may be involved in water transport at the choroid plexus and blood-brain barrier (BBB) in the brain. The expression of AQP4, another brain AQP expressed at the BBB, was decreased by half in AQP11-deficient mice, thereby suggesting the presence of the interaction between AQP11 and AQP4. The brain of AQP11-deficient mice, however, did not show any morphological abnormalities and the function of the BBB was intact. Our findings provide a novel insight into a water transport mechanism mediated by AQPs in the brain, which may lead to a new therapy for brain edema. |
| first_indexed | 2024-04-13T00:33:52Z |
| format | Article |
| id | doaj.art-dc846949977844b88f452df513f1cf10 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T00:33:52Z |
| publishDate | 2016-06-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dc846949977844b88f452df513f1cf102022-12-22T03:10:24ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-06-0117686110.3390/ijms17060861ijms17060861Aquaporin-11 (AQP11) Expression in the Mouse BrainShin Koike0Yasuko Tanaka1Toshiyuki Matsuzaki2Yoshiyuki Morishita3Kenichi Ishibashi4Division of Pathophysiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, JapanDivision of Pathophysiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, JapanDepartment of Anatomy and Cell Biology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, JapanDivision of Nephrology, Saitama Medical Center, Jichi Medical University, 1-847 Ohmiya, Saitama-City, Saitama 330-8503, JapanDivision of Pathophysiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, JapanAquaporin-11 (AQP11) is an intracellular aquaporin expressed in various tissues, including brain tissues in mammals. While AQP11-deficient mice have developed fatal polycystic kidneys at one month old, the role of AQP11 in the brain was not well appreciated. In this study, we examined the AQP11 expression in the mouse brain and the brain phenotype of AQP11-deficient mice. AQP11 messenger ribonucleic acid (mRNA) and protein were expressed in the brain, but much less than in the thymus and kidney. Immunostaining showed that AQP11 was localized at the epithelium of the choroid plexus and at the endothelium of the brain capillary, suggesting that AQP11 may be involved in water transport at the choroid plexus and blood-brain barrier (BBB) in the brain. The expression of AQP4, another brain AQP expressed at the BBB, was decreased by half in AQP11-deficient mice, thereby suggesting the presence of the interaction between AQP11 and AQP4. The brain of AQP11-deficient mice, however, did not show any morphological abnormalities and the function of the BBB was intact. Our findings provide a novel insight into a water transport mechanism mediated by AQPs in the brain, which may lead to a new therapy for brain edema.http://www.mdpi.com/1422-0067/17/6/861blood brain barrierbrain edemaAQP4choroid plexuspia mater |
| spellingShingle | Shin Koike Yasuko Tanaka Toshiyuki Matsuzaki Yoshiyuki Morishita Kenichi Ishibashi Aquaporin-11 (AQP11) Expression in the Mouse Brain International Journal of Molecular Sciences blood brain barrier brain edema AQP4 choroid plexus pia mater |
| title | Aquaporin-11 (AQP11) Expression in the Mouse Brain |
| title_full | Aquaporin-11 (AQP11) Expression in the Mouse Brain |
| title_fullStr | Aquaporin-11 (AQP11) Expression in the Mouse Brain |
| title_full_unstemmed | Aquaporin-11 (AQP11) Expression in the Mouse Brain |
| title_short | Aquaporin-11 (AQP11) Expression in the Mouse Brain |
| title_sort | aquaporin 11 aqp11 expression in the mouse brain |
| topic | blood brain barrier brain edema AQP4 choroid plexus pia mater |
| url | http://www.mdpi.com/1422-0067/17/6/861 |
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