Comparative Analysis of Milk Microbiomes and Their Association with Bovine Mastitis in Two Farms in Central Russia
Bovine mastitis is a widespread infectious disease. In addition to the economic damages associated with reduced milk yield due to mastitis, the problem of food contamination by microorganism metabolites, in particular toxins, is also a concern. Horizontal transfer of microorganisms from animal popul...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/11/5/1401 |
| _version_ | 1797534128008069120 |
|---|---|
| author | Sergei Sokolov Ksenia Fursova Irina Shulcheva Daria Nikanova Olga Artyemieva Evgenia Kolodina Anatoly Sorokin Timur Dzhelyadin Margarita Shchannikova Anna Shepelyakovskaya Natalia Zinovieva Fedor Brovko |
| author_facet | Sergei Sokolov Ksenia Fursova Irina Shulcheva Daria Nikanova Olga Artyemieva Evgenia Kolodina Anatoly Sorokin Timur Dzhelyadin Margarita Shchannikova Anna Shepelyakovskaya Natalia Zinovieva Fedor Brovko |
| author_sort | Sergei Sokolov |
| collection | DOAJ |
| description | Bovine mastitis is a widespread infectious disease. In addition to the economic damages associated with reduced milk yield due to mastitis, the problem of food contamination by microorganism metabolites, in particular toxins, is also a concern. Horizontal transfer of microorganisms from animal populations to humans can also be complicated by antibiotic resistance. Therefore, bovine mastitis is relevant to the study of microbiology and veterinary medicine. In this study, we investigated the microbiome of milk samples from healthy cows and cows with different forms of mastitis from individual quarters of the udder of cows during first and second lactation. Total DNA was extracted from milk samples. The V3–V4 regions of the bacterial 16S rRNA genes from each sample were amplified to generate a library via high-throughput sequencing. We revealed significant dominance of several operational taxonomic units (OTUs) corresponding mostly to groups of <i>Staphylococcus aureus</i>, <i>Aerococcus</i> spp., and <i>Streptococcus</i> spp. In addition, we unexpectedly identified <i>Streptococcus thermophilus</i> in samples with high SCC quantities. We found some infectious agents that characterized summer mastitis. We demonstrated that in Central Russia, mastitis is associated with a wide variety of causal organisms. We observed some differences in the diversity of the two investigated farms. However, we did not find any significant difference among healthy, mastitis and subclinical samples according to their SCC status from either farms by principal component analysis. Linear discriminant analysis effect size (LEfSe) confirmed the presence of several indicator genera in farms from Moscow and the Tula Region. These results confirm the complex bacterial etiology of bovine mastitis. |
| first_indexed | 2024-03-10T11:25:15Z |
| format | Article |
| id | doaj.art-dc848fd016514bd7b01bbe2f18a98b51 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T11:25:15Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-dc848fd016514bd7b01bbe2f18a98b512023-11-21T19:43:54ZengMDPI AGAnimals2076-26152021-05-01115140110.3390/ani11051401Comparative Analysis of Milk Microbiomes and Their Association with Bovine Mastitis in Two Farms in Central RussiaSergei Sokolov0Ksenia Fursova1Irina Shulcheva2Daria Nikanova3Olga Artyemieva4Evgenia Kolodina5Anatoly Sorokin6Timur Dzhelyadin7Margarita Shchannikova8Anna Shepelyakovskaya9Natalia Zinovieva10Fedor Brovko11Laboratory of Microbiology, L.K. Ernst Federal Science Center for Animal Husbandry, Dubrovitsy 142132, RussiaLaboratory of Immunochemistry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino 142290, RussiaLaboratory of Immunochemistry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino 142290, RussiaLaboratory of Microbiology, L.K. Ernst Federal Science Center for Animal Husbandry, Dubrovitsy 142132, RussiaLaboratory of Microbiology, L.K. Ernst Federal Science Center for Animal Husbandry, Dubrovitsy 142132, RussiaLaboratory of Microbiology, L.K. Ernst Federal Science Center for Animal Husbandry, Dubrovitsy 142132, RussiaLaboratory of Cell Genome Functioning Mechanisms, Federal Research Center “Pushchino Scientific Center for Biological Researches”, Institute of Cell Biophysics of the Russian Academy of Sciences, Pushchino 142290, RussiaLaboratory of Cell Genome Functioning Mechanisms, Federal Research Center “Pushchino Scientific Center for Biological Researches”, Institute of Cell Biophysics of the Russian Academy of Sciences, Pushchino 142290, RussiaLaboratory of Immunochemistry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino 142290, RussiaLaboratory of Immunochemistry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino 142290, RussiaLaboratory of Microbiology, L.K. Ernst Federal Science Center for Animal Husbandry, Dubrovitsy 142132, RussiaLaboratory of Microbiology, L.K. Ernst Federal Science Center for Animal Husbandry, Dubrovitsy 142132, RussiaBovine mastitis is a widespread infectious disease. In addition to the economic damages associated with reduced milk yield due to mastitis, the problem of food contamination by microorganism metabolites, in particular toxins, is also a concern. Horizontal transfer of microorganisms from animal populations to humans can also be complicated by antibiotic resistance. Therefore, bovine mastitis is relevant to the study of microbiology and veterinary medicine. In this study, we investigated the microbiome of milk samples from healthy cows and cows with different forms of mastitis from individual quarters of the udder of cows during first and second lactation. Total DNA was extracted from milk samples. The V3–V4 regions of the bacterial 16S rRNA genes from each sample were amplified to generate a library via high-throughput sequencing. We revealed significant dominance of several operational taxonomic units (OTUs) corresponding mostly to groups of <i>Staphylococcus aureus</i>, <i>Aerococcus</i> spp., and <i>Streptococcus</i> spp. In addition, we unexpectedly identified <i>Streptococcus thermophilus</i> in samples with high SCC quantities. We found some infectious agents that characterized summer mastitis. We demonstrated that in Central Russia, mastitis is associated with a wide variety of causal organisms. We observed some differences in the diversity of the two investigated farms. However, we did not find any significant difference among healthy, mastitis and subclinical samples according to their SCC status from either farms by principal component analysis. Linear discriminant analysis effect size (LEfSe) confirmed the presence of several indicator genera in farms from Moscow and the Tula Region. These results confirm the complex bacterial etiology of bovine mastitis.https://www.mdpi.com/2076-2615/11/5/1401bovine mastitismilk microbiomemicrobial diversity<i>Staphylococcus</i><i>Aerococcus</i>Central Russia |
| spellingShingle | Sergei Sokolov Ksenia Fursova Irina Shulcheva Daria Nikanova Olga Artyemieva Evgenia Kolodina Anatoly Sorokin Timur Dzhelyadin Margarita Shchannikova Anna Shepelyakovskaya Natalia Zinovieva Fedor Brovko Comparative Analysis of Milk Microbiomes and Their Association with Bovine Mastitis in Two Farms in Central Russia Animals bovine mastitis milk microbiome microbial diversity <i>Staphylococcus</i> <i>Aerococcus</i> Central Russia |
| title | Comparative Analysis of Milk Microbiomes and Their Association with Bovine Mastitis in Two Farms in Central Russia |
| title_full | Comparative Analysis of Milk Microbiomes and Their Association with Bovine Mastitis in Two Farms in Central Russia |
| title_fullStr | Comparative Analysis of Milk Microbiomes and Their Association with Bovine Mastitis in Two Farms in Central Russia |
| title_full_unstemmed | Comparative Analysis of Milk Microbiomes and Their Association with Bovine Mastitis in Two Farms in Central Russia |
| title_short | Comparative Analysis of Milk Microbiomes and Their Association with Bovine Mastitis in Two Farms in Central Russia |
| title_sort | comparative analysis of milk microbiomes and their association with bovine mastitis in two farms in central russia |
| topic | bovine mastitis milk microbiome microbial diversity <i>Staphylococcus</i> <i>Aerococcus</i> Central Russia |
| url | https://www.mdpi.com/2076-2615/11/5/1401 |
| work_keys_str_mv | AT sergeisokolov comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia AT kseniafursova comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia AT irinashulcheva comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia AT darianikanova comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia AT olgaartyemieva comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia AT evgeniakolodina comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia AT anatolysorokin comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia AT timurdzhelyadin comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia AT margaritashchannikova comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia AT annashepelyakovskaya comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia AT nataliazinovieva comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia AT fedorbrovko comparativeanalysisofmilkmicrobiomesandtheirassociationwithbovinemastitisintwofarmsincentralrussia |