Iron Sulfide Enhanced the Dechlorination of Trichloroethene by Dehalococcoides mccartyi Strain 195
Iron sulfide (FeS) nanoparticles have great potential in environmental remediation. Using the representative species Dehalococcoides mccartyi strain 195 (Dhc 195), the effect of FeS on trichloroethene (TCE) dechlorination was studied with hydrogen and acetate as the electron donor and carbon source,...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.665281/full |
| _version_ | 1818730854797541376 |
|---|---|
| author | Yaru Li Yaru Li He-Ping Zhao Lizhong Zhu Lizhong Zhu |
| author_facet | Yaru Li Yaru Li He-Ping Zhao Lizhong Zhu Lizhong Zhu |
| author_sort | Yaru Li |
| collection | DOAJ |
| description | Iron sulfide (FeS) nanoparticles have great potential in environmental remediation. Using the representative species Dehalococcoides mccartyi strain 195 (Dhc 195), the effect of FeS on trichloroethene (TCE) dechlorination was studied with hydrogen and acetate as the electron donor and carbon source, respectively. With the addition of 0.2 mM Fe2+ and S2–, the dechlorination rate of TCE was enhanced from 25.46 ± 1.15 to 37.84 ± 1.89 μmol⋅L–1⋅day–1 by the in situ formed FeS nanoparticles, as revealed through X-ray diffraction. Comparing the tceA gene copy numbers between with FeS and without FeS, real-time polymerase chain reaction (PCR) indicated that the abundance of the tceA gene increased from (2.83 ± 0.13) × 107 to (4.27 ± 0.21) × 108 copies/ml on day 12. The transcriptional activity of key genes involved in the electron transport chain was upregulated after the addition of FeS, including those responsible for the iron–sulfur cluster assembly protein gene (DET1632) and transmembrane transport of iron (DET1503, DET0685), cobalamin (DET0685, DET1139), and molybdenum (DET1161) genes. Meanwhile, the reverse transcription of tceA was increased approximately five times on the 12th day. These upregulations together suggested that the electron transport of D. mccartyi strain 195 was enhanced by FeS for apparent TCE dechlorination. Overall, the present study provided an eco-friendly and effective method to achieve high remediation efficiency for organohalide-polluted groundwater and soil. |
| first_indexed | 2024-12-17T23:08:24Z |
| format | Article |
| id | doaj.art-dc8ef454208d4194b50b861d114ef902 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T23:08:24Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-dc8ef454208d4194b50b861d114ef9022022-12-21T21:29:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-06-011210.3389/fmicb.2021.665281665281Iron Sulfide Enhanced the Dechlorination of Trichloroethene by Dehalococcoides mccartyi Strain 195Yaru Li0Yaru Li1He-Ping Zhao2Lizhong Zhu3Lizhong Zhu4College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, ChinaKey Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou, ChinaCollege of Environmental and Resource Sciences, Zhejiang University, Hangzhou, ChinaCollege of Environmental and Resource Sciences, Zhejiang University, Hangzhou, ChinaKey Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou, ChinaIron sulfide (FeS) nanoparticles have great potential in environmental remediation. Using the representative species Dehalococcoides mccartyi strain 195 (Dhc 195), the effect of FeS on trichloroethene (TCE) dechlorination was studied with hydrogen and acetate as the electron donor and carbon source, respectively. With the addition of 0.2 mM Fe2+ and S2–, the dechlorination rate of TCE was enhanced from 25.46 ± 1.15 to 37.84 ± 1.89 μmol⋅L–1⋅day–1 by the in situ formed FeS nanoparticles, as revealed through X-ray diffraction. Comparing the tceA gene copy numbers between with FeS and without FeS, real-time polymerase chain reaction (PCR) indicated that the abundance of the tceA gene increased from (2.83 ± 0.13) × 107 to (4.27 ± 0.21) × 108 copies/ml on day 12. The transcriptional activity of key genes involved in the electron transport chain was upregulated after the addition of FeS, including those responsible for the iron–sulfur cluster assembly protein gene (DET1632) and transmembrane transport of iron (DET1503, DET0685), cobalamin (DET0685, DET1139), and molybdenum (DET1161) genes. Meanwhile, the reverse transcription of tceA was increased approximately five times on the 12th day. These upregulations together suggested that the electron transport of D. mccartyi strain 195 was enhanced by FeS for apparent TCE dechlorination. Overall, the present study provided an eco-friendly and effective method to achieve high remediation efficiency for organohalide-polluted groundwater and soil.https://www.frontiersin.org/articles/10.3389/fmicb.2021.665281/fullDehalococcoides mccartyi strain 195FeStrichloroetheneelectron transportiron-sulfur cluster |
| spellingShingle | Yaru Li Yaru Li He-Ping Zhao Lizhong Zhu Lizhong Zhu Iron Sulfide Enhanced the Dechlorination of Trichloroethene by Dehalococcoides mccartyi Strain 195 Frontiers in Microbiology Dehalococcoides mccartyi strain 195 FeS trichloroethene electron transport iron-sulfur cluster |
| title | Iron Sulfide Enhanced the Dechlorination of Trichloroethene by Dehalococcoides mccartyi Strain 195 |
| title_full | Iron Sulfide Enhanced the Dechlorination of Trichloroethene by Dehalococcoides mccartyi Strain 195 |
| title_fullStr | Iron Sulfide Enhanced the Dechlorination of Trichloroethene by Dehalococcoides mccartyi Strain 195 |
| title_full_unstemmed | Iron Sulfide Enhanced the Dechlorination of Trichloroethene by Dehalococcoides mccartyi Strain 195 |
| title_short | Iron Sulfide Enhanced the Dechlorination of Trichloroethene by Dehalococcoides mccartyi Strain 195 |
| title_sort | iron sulfide enhanced the dechlorination of trichloroethene by dehalococcoides mccartyi strain 195 |
| topic | Dehalococcoides mccartyi strain 195 FeS trichloroethene electron transport iron-sulfur cluster |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.665281/full |
| work_keys_str_mv | AT yaruli ironsulfideenhancedthedechlorinationoftrichloroethenebydehalococcoidesmccartyistrain195 AT yaruli ironsulfideenhancedthedechlorinationoftrichloroethenebydehalococcoidesmccartyistrain195 AT hepingzhao ironsulfideenhancedthedechlorinationoftrichloroethenebydehalococcoidesmccartyistrain195 AT lizhongzhu ironsulfideenhancedthedechlorinationoftrichloroethenebydehalococcoidesmccartyistrain195 AT lizhongzhu ironsulfideenhancedthedechlorinationoftrichloroethenebydehalococcoidesmccartyistrain195 |