Detection of BRAF Gene Mutation in Preoperative Diagnostic of Thyroid Gland Cancer
Purpose: To assess the utility of BRAFV600E mutation detection in preoperative thyroid cancer diagnostic. Material and methods: We studied 46 aspirates taken by FNA from patients with thyroid gland nodes. Thyroid tissue aspirates DNA was extracted by sorbent method. BRAF gene mutation was analyzed w...
| Format: | Article |
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| Language: | Russian |
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Endocrinology Research Centre
2009-12-01
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| Series: | Клиническая и экспериментальная тиреоидология |
| Subjects: | |
| Online Access: | https://cet-endojournals.ru/ket/article/viewFile/4446/2573 |
| _version_ | 1819009985570406400 |
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| collection | DOAJ |
| description | Purpose: To assess the utility of BRAFV600E mutation detection in preoperative thyroid cancer diagnostic. Material and methods: We studied 46 aspirates taken by FNA from patients with thyroid gland nodes. Thyroid tissue aspirates DNA was extracted by sorbent method. BRAF gene mutation was analyzed with primers specific for wild and mutant gen type by RT-PCR. Results: All patients were divided into three groups by cytological conclusions: colloid nodules (9), PTC (19), and suspicious for malignancy (18). In the group of patients with PTC all diagnoses were confirmed by histology, and BRAF gene mutation was detected in 15 (79%) FNAB specimens. In the group with suspicious cytological diagnosis only one patient had follicular cancer by histology and positive BRAF mutation. There were detected no BRAF mutation in 16 patients with histologically proven follicular adenoma, in 9 patients with colloid nodular goiter and in one patient with follicular cancer. Thereby, we received the following criteria valuers of method’s reliability: sensitivity – 76%, specificity – 100%, diagnostic accuracy – 89%. Conclusions: Detection of the BRAFV600E mutation may be a useful adjunct marker for preoperative diagnostic of thyroid gland cancer. |
| first_indexed | 2024-12-21T01:05:04Z |
| format | Article |
| id | doaj.art-dc943463c97c4d309ecb4d21d4f9c925 |
| institution | Directory Open Access Journal |
| issn | 1995-5472 2310-3787 |
| language | Russian |
| last_indexed | 2024-12-21T01:05:04Z |
| publishDate | 2009-12-01 |
| publisher | Endocrinology Research Centre |
| record_format | Article |
| series | Клиническая и экспериментальная тиреоидология |
| spelling | doaj.art-dc943463c97c4d309ecb4d21d4f9c9252022-12-21T19:21:04ZrusEndocrinology Research CentreКлиническая и экспериментальная тиреоидология1995-54722310-37872009-12-0154333710.14341/ket20095433-374404Detection of BRAF Gene Mutation in Preoperative Diagnostic of Thyroid Gland CancerPurpose: To assess the utility of BRAFV600E mutation detection in preoperative thyroid cancer diagnostic. Material and methods: We studied 46 aspirates taken by FNA from patients with thyroid gland nodes. Thyroid tissue aspirates DNA was extracted by sorbent method. BRAF gene mutation was analyzed with primers specific for wild and mutant gen type by RT-PCR. Results: All patients were divided into three groups by cytological conclusions: colloid nodules (9), PTC (19), and suspicious for malignancy (18). In the group of patients with PTC all diagnoses were confirmed by histology, and BRAF gene mutation was detected in 15 (79%) FNAB specimens. In the group with suspicious cytological diagnosis only one patient had follicular cancer by histology and positive BRAF mutation. There were detected no BRAF mutation in 16 patients with histologically proven follicular adenoma, in 9 patients with colloid nodular goiter and in one patient with follicular cancer. Thereby, we received the following criteria valuers of method’s reliability: sensitivity – 76%, specificity – 100%, diagnostic accuracy – 89%. Conclusions: Detection of the BRAFV600E mutation may be a useful adjunct marker for preoperative diagnostic of thyroid gland cancer.https://cet-endojournals.ru/ket/article/viewFile/4446/2573thyroidnodular goiterthyroid cancerfollicular lesion |
| spellingShingle | Detection of BRAF Gene Mutation in Preoperative Diagnostic of Thyroid Gland Cancer Клиническая и экспериментальная тиреоидология thyroid nodular goiter thyroid cancer follicular lesion |
| title | Detection of BRAF Gene Mutation in Preoperative Diagnostic of Thyroid Gland Cancer |
| title_full | Detection of BRAF Gene Mutation in Preoperative Diagnostic of Thyroid Gland Cancer |
| title_fullStr | Detection of BRAF Gene Mutation in Preoperative Diagnostic of Thyroid Gland Cancer |
| title_full_unstemmed | Detection of BRAF Gene Mutation in Preoperative Diagnostic of Thyroid Gland Cancer |
| title_short | Detection of BRAF Gene Mutation in Preoperative Diagnostic of Thyroid Gland Cancer |
| title_sort | detection of braf gene mutation in preoperative diagnostic of thyroid gland cancer |
| topic | thyroid nodular goiter thyroid cancer follicular lesion |
| url | https://cet-endojournals.ru/ket/article/viewFile/4446/2573 |