Plasmids manipulate bacterial behaviour through translational regulatory crosstalk.
Beyond their role in horizontal gene transfer, conjugative plasmids commonly encode homologues of bacterial regulators. Known plasmid regulator homologues have highly targeted effects upon the transcription of specific bacterial traits. Here, we characterise a plasmid translational regulator, RsmQ,...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-02-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.3001988 |
| _version_ | 1828004349799825408 |
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| author | Catriona M A Thompson James P J Hall Govind Chandra Carlo Martins Gerhard Saalbach Supakan Panturat Susannah M Bird Samuel Ford Richard H Little Ainelen Piazza Ellie Harrison Robert W Jackson Michael A Brockhurst Jacob G Malone |
| author_facet | Catriona M A Thompson James P J Hall Govind Chandra Carlo Martins Gerhard Saalbach Supakan Panturat Susannah M Bird Samuel Ford Richard H Little Ainelen Piazza Ellie Harrison Robert W Jackson Michael A Brockhurst Jacob G Malone |
| author_sort | Catriona M A Thompson |
| collection | DOAJ |
| description | Beyond their role in horizontal gene transfer, conjugative plasmids commonly encode homologues of bacterial regulators. Known plasmid regulator homologues have highly targeted effects upon the transcription of specific bacterial traits. Here, we characterise a plasmid translational regulator, RsmQ, capable of taking global regulatory control in Pseudomonas fluorescens and causing a behavioural switch from motile to sessile lifestyle. RsmQ acts as a global regulator, controlling the host proteome through direct interaction with host mRNAs and interference with the host's translational regulatory network. This mRNA interference leads to large-scale proteomic changes in metabolic genes, key regulators, and genes involved in chemotaxis, thus controlling bacterial metabolism and motility. Moreover, comparative analyses found RsmQ to be encoded on a large number of divergent plasmids isolated from multiple bacterial host taxa, suggesting the widespread importance of RsmQ for manipulating bacterial behaviour across clinical, environmental, and agricultural niches. RsmQ is a widespread plasmid global translational regulator primarily evolved for host chromosomal control to manipulate bacterial behaviour and lifestyle. |
| first_indexed | 2024-04-10T07:13:25Z |
| format | Article |
| id | doaj.art-dc9504425e3e41edab5b57370e5fa862 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-04-10T07:13:25Z |
| publishDate | 2023-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-dc9504425e3e41edab5b57370e5fa8622023-02-26T05:30:47ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852023-02-01212e300198810.1371/journal.pbio.3001988Plasmids manipulate bacterial behaviour through translational regulatory crosstalk.Catriona M A ThompsonJames P J HallGovind ChandraCarlo MartinsGerhard SaalbachSupakan PanturatSusannah M BirdSamuel FordRichard H LittleAinelen PiazzaEllie HarrisonRobert W JacksonMichael A BrockhurstJacob G MaloneBeyond their role in horizontal gene transfer, conjugative plasmids commonly encode homologues of bacterial regulators. Known plasmid regulator homologues have highly targeted effects upon the transcription of specific bacterial traits. Here, we characterise a plasmid translational regulator, RsmQ, capable of taking global regulatory control in Pseudomonas fluorescens and causing a behavioural switch from motile to sessile lifestyle. RsmQ acts as a global regulator, controlling the host proteome through direct interaction with host mRNAs and interference with the host's translational regulatory network. This mRNA interference leads to large-scale proteomic changes in metabolic genes, key regulators, and genes involved in chemotaxis, thus controlling bacterial metabolism and motility. Moreover, comparative analyses found RsmQ to be encoded on a large number of divergent plasmids isolated from multiple bacterial host taxa, suggesting the widespread importance of RsmQ for manipulating bacterial behaviour across clinical, environmental, and agricultural niches. RsmQ is a widespread plasmid global translational regulator primarily evolved for host chromosomal control to manipulate bacterial behaviour and lifestyle.https://doi.org/10.1371/journal.pbio.3001988 |
| spellingShingle | Catriona M A Thompson James P J Hall Govind Chandra Carlo Martins Gerhard Saalbach Supakan Panturat Susannah M Bird Samuel Ford Richard H Little Ainelen Piazza Ellie Harrison Robert W Jackson Michael A Brockhurst Jacob G Malone Plasmids manipulate bacterial behaviour through translational regulatory crosstalk. PLoS Biology |
| title | Plasmids manipulate bacterial behaviour through translational regulatory crosstalk. |
| title_full | Plasmids manipulate bacterial behaviour through translational regulatory crosstalk. |
| title_fullStr | Plasmids manipulate bacterial behaviour through translational regulatory crosstalk. |
| title_full_unstemmed | Plasmids manipulate bacterial behaviour through translational regulatory crosstalk. |
| title_short | Plasmids manipulate bacterial behaviour through translational regulatory crosstalk. |
| title_sort | plasmids manipulate bacterial behaviour through translational regulatory crosstalk |
| url | https://doi.org/10.1371/journal.pbio.3001988 |
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