Genomic and Transcriptomic Analysis of Pea (<i>Pisum sativum</i> L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity
Pea (<i>Pisum sativum</i> L.), like most legumes, forms mutualistic symbioses with nodule bacteria and arbuscular mycorrhizal (AM) fungi. The positive effect of inoculation is partially determined by the plant genotype; thus, pea varieties with high and low symbiotic responsivity have be...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/1/78 |
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| author | Evgeny A. Zorin Anton S. Sulima Aleksandr I. Zhernakov Daria O. Kuzmina Valeria A. Rakova Marina S. Kliukova Daria A. Romanyuk Olga A. Kulaeva Gulnar A. Akhtemova Oksana Y. Shtark Igor A. Tikhonovich Vladimir A. Zhukov |
| author_facet | Evgeny A. Zorin Anton S. Sulima Aleksandr I. Zhernakov Daria O. Kuzmina Valeria A. Rakova Marina S. Kliukova Daria A. Romanyuk Olga A. Kulaeva Gulnar A. Akhtemova Oksana Y. Shtark Igor A. Tikhonovich Vladimir A. Zhukov |
| author_sort | Evgeny A. Zorin |
| collection | DOAJ |
| description | Pea (<i>Pisum sativum</i> L.), like most legumes, forms mutualistic symbioses with nodule bacteria and arbuscular mycorrhizal (AM) fungi. The positive effect of inoculation is partially determined by the plant genotype; thus, pea varieties with high and low symbiotic responsivity have been described, but the molecular genetic basis of this trait remains unknown. Here, we compare the symbiotically responsive breeding line ‘Triumph’ of grain pea with its parental cultivars ‘Vendevil’ (a donor of high symbiotic responsivity) and ‘Classic’ (a donor of agriculturally valuable traits) using genome and transcriptome sequencing. We show that ‘Triumph’ inherited one-fourth of its genome from ‘Vendevil’, including the genes related to AM and nodule formation, and reveal that under combined inoculation with nodule bacteria and AM fungi, ‘Triumph’ and ‘Vendevil’, in contrast to ‘Classic’, demonstrate similar up-regulation of the genes related to solute transport, hormonal regulation and flavonoid biosynthesis in their roots. We also identify the gene <i>PsGLP2</i>, whose expression pattern distinguishing ‘Triumph’ and ‘Vendevil’ from ‘Classic’ correlates with difference within the promoter region sequence, making it a promising marker for the symbiotic responsivity trait. The results of this study may be helpful for future molecular breeding programs aimed at creation of symbiotically responsive cultivars of pea. |
| first_indexed | 2024-03-08T14:59:47Z |
| format | Article |
| id | doaj.art-dc96a61787af439693ecfa7d216126ad |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T14:59:47Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-dc96a61787af439693ecfa7d216126ad2024-01-10T15:06:12ZengMDPI AGPlants2223-77472023-12-011317810.3390/plants13010078Genomic and Transcriptomic Analysis of Pea (<i>Pisum sativum</i> L.) Breeding Line ‘Triumph’ with High Symbiotic ResponsivityEvgeny A. Zorin0Anton S. Sulima1Aleksandr I. Zhernakov2Daria O. Kuzmina3Valeria A. Rakova4Marina S. Kliukova5Daria A. Romanyuk6Olga A. Kulaeva7Gulnar A. Akhtemova8Oksana Y. Shtark9Igor A. Tikhonovich10Vladimir A. Zhukov11All-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg 196608, RussiaCenter of Genetics and Life Sciences, Sirius University of Science and Technology, Sirius 354340, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg 196608, RussiaPea (<i>Pisum sativum</i> L.), like most legumes, forms mutualistic symbioses with nodule bacteria and arbuscular mycorrhizal (AM) fungi. The positive effect of inoculation is partially determined by the plant genotype; thus, pea varieties with high and low symbiotic responsivity have been described, but the molecular genetic basis of this trait remains unknown. Here, we compare the symbiotically responsive breeding line ‘Triumph’ of grain pea with its parental cultivars ‘Vendevil’ (a donor of high symbiotic responsivity) and ‘Classic’ (a donor of agriculturally valuable traits) using genome and transcriptome sequencing. We show that ‘Triumph’ inherited one-fourth of its genome from ‘Vendevil’, including the genes related to AM and nodule formation, and reveal that under combined inoculation with nodule bacteria and AM fungi, ‘Triumph’ and ‘Vendevil’, in contrast to ‘Classic’, demonstrate similar up-regulation of the genes related to solute transport, hormonal regulation and flavonoid biosynthesis in their roots. We also identify the gene <i>PsGLP2</i>, whose expression pattern distinguishing ‘Triumph’ and ‘Vendevil’ from ‘Classic’ correlates with difference within the promoter region sequence, making it a promising marker for the symbiotic responsivity trait. The results of this study may be helpful for future molecular breeding programs aimed at creation of symbiotically responsive cultivars of pea.https://www.mdpi.com/2223-7747/13/1/78plant–microbe symbiosislegumes<i>Pisum sativum</i>symbiotic responsivitygenomicstranscriptomics |
| spellingShingle | Evgeny A. Zorin Anton S. Sulima Aleksandr I. Zhernakov Daria O. Kuzmina Valeria A. Rakova Marina S. Kliukova Daria A. Romanyuk Olga A. Kulaeva Gulnar A. Akhtemova Oksana Y. Shtark Igor A. Tikhonovich Vladimir A. Zhukov Genomic and Transcriptomic Analysis of Pea (<i>Pisum sativum</i> L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity Plants plant–microbe symbiosis legumes <i>Pisum sativum</i> symbiotic responsivity genomics transcriptomics |
| title | Genomic and Transcriptomic Analysis of Pea (<i>Pisum sativum</i> L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity |
| title_full | Genomic and Transcriptomic Analysis of Pea (<i>Pisum sativum</i> L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity |
| title_fullStr | Genomic and Transcriptomic Analysis of Pea (<i>Pisum sativum</i> L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity |
| title_full_unstemmed | Genomic and Transcriptomic Analysis of Pea (<i>Pisum sativum</i> L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity |
| title_short | Genomic and Transcriptomic Analysis of Pea (<i>Pisum sativum</i> L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity |
| title_sort | genomic and transcriptomic analysis of pea i pisum sativum i l breeding line triumph with high symbiotic responsivity |
| topic | plant–microbe symbiosis legumes <i>Pisum sativum</i> symbiotic responsivity genomics transcriptomics |
| url | https://www.mdpi.com/2223-7747/13/1/78 |
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