Nematode surface functionalization with hydrogel sheaths tailored in situ
Engineering the surfaces of biological organisms allows the introduction of novel functions and enhances their native functions. However, studies on surface engineering remained limited to unicellular organisms. Herein, nematode surfaces are engineered through in situ hydrogelation mediated by horse...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422001260 |
| _version_ | 1828519791467429888 |
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| author | Wildan Mubarok Masaki Nakahata Masaru Kojima Shinji Sakai |
| author_facet | Wildan Mubarok Masaki Nakahata Masaru Kojima Shinji Sakai |
| author_sort | Wildan Mubarok |
| collection | DOAJ |
| description | Engineering the surfaces of biological organisms allows the introduction of novel functions and enhances their native functions. However, studies on surface engineering remained limited to unicellular organisms. Herein, nematode surfaces are engineered through in situ hydrogelation mediated by horseradish peroxidase (HRP) anchored to nematode cuticles. With this method, hydrogel sheaths of approximately 10-μm thickness are fabricated from a variety of polysaccharides, proteins, and synthetic polymers. Caenorhabditis elegans and Anisakis simplex coated with a hydrogel sheath showed a negligible decrease in viability, chemotaxis and locomotion. Hydrogel sheaths containing UV-absorbable groups and catalase functioned as shields to protect nematodes from UV and hydrogen peroxide, respectively. The results also showed that hydrogel sheaths containing glucose oxidase have the potential to be used as living drug delivery systems for cancer therapy. The nematode functionalization method developed in this study has the potential to impact a wide range of fields from agriculture to medicine. |
| first_indexed | 2024-12-11T19:19:25Z |
| format | Article |
| id | doaj.art-dc9b7cf90fd24f728671639e6459d863 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-12-11T19:19:25Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-dc9b7cf90fd24f728671639e6459d8632022-12-22T00:53:34ZengElsevierMaterials Today Bio2590-00642022-06-0115100328Nematode surface functionalization with hydrogel sheaths tailored in situWildan Mubarok0Masaki Nakahata1Masaru Kojima2Shinji Sakai3Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Osaka, 560-8531, JapanDivision of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Osaka, 560-8531, JapanDivision of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Osaka, 560-8531, JapanCorresponding author.; Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Osaka, 560-8531, JapanEngineering the surfaces of biological organisms allows the introduction of novel functions and enhances their native functions. However, studies on surface engineering remained limited to unicellular organisms. Herein, nematode surfaces are engineered through in situ hydrogelation mediated by horseradish peroxidase (HRP) anchored to nematode cuticles. With this method, hydrogel sheaths of approximately 10-μm thickness are fabricated from a variety of polysaccharides, proteins, and synthetic polymers. Caenorhabditis elegans and Anisakis simplex coated with a hydrogel sheath showed a negligible decrease in viability, chemotaxis and locomotion. Hydrogel sheaths containing UV-absorbable groups and catalase functioned as shields to protect nematodes from UV and hydrogen peroxide, respectively. The results also showed that hydrogel sheaths containing glucose oxidase have the potential to be used as living drug delivery systems for cancer therapy. The nematode functionalization method developed in this study has the potential to impact a wide range of fields from agriculture to medicine.http://www.sciencedirect.com/science/article/pii/S2590006422001260Surface functionalizationCaenorhabditis elegansAnisakis simplexHydrogel sheathLiving drug delivery systemHorseradish peroxidase |
| spellingShingle | Wildan Mubarok Masaki Nakahata Masaru Kojima Shinji Sakai Nematode surface functionalization with hydrogel sheaths tailored in situ Materials Today Bio Surface functionalization Caenorhabditis elegans Anisakis simplex Hydrogel sheath Living drug delivery system Horseradish peroxidase |
| title | Nematode surface functionalization with hydrogel sheaths tailored in situ |
| title_full | Nematode surface functionalization with hydrogel sheaths tailored in situ |
| title_fullStr | Nematode surface functionalization with hydrogel sheaths tailored in situ |
| title_full_unstemmed | Nematode surface functionalization with hydrogel sheaths tailored in situ |
| title_short | Nematode surface functionalization with hydrogel sheaths tailored in situ |
| title_sort | nematode surface functionalization with hydrogel sheaths tailored in situ |
| topic | Surface functionalization Caenorhabditis elegans Anisakis simplex Hydrogel sheath Living drug delivery system Horseradish peroxidase |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422001260 |
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