Population Genetic Structure of Three Cichlids in Ilorin, North-Central Nigeria
Cichlids are among the economically important which serve as a source of food for people around the world. A deep understanding of the population structure and genetic diversity of cichlids are vital for initiation of conservation policies and sustainable aquaculture. There is paucity of...
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| Format: | Article |
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University of Baghdad, College of Veterinary Medicine
2022-06-01
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| Series: | The Iraqi Journal of Veterinary Medicine |
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| Online Access: | https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1307 |
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| author | Oluyinka A Iyiola Rahmat D Shaibu Segun O Oladipo Kazeem O Kareem Lotanna M Nneji Adeniyi C Adeola Abass T Anifowoshe Moise M Matouke |
| author_facet | Oluyinka A Iyiola Rahmat D Shaibu Segun O Oladipo Kazeem O Kareem Lotanna M Nneji Adeniyi C Adeola Abass T Anifowoshe Moise M Matouke |
| author_sort | Oluyinka A Iyiola |
| collection | DOAJ |
| description |
Cichlids are among the economically important which serve as a source of food for people around the world. A deep understanding of the population structure and genetic diversity of cichlids are vital for initiation of conservation policies and sustainable aquaculture. There is paucity of information on the patterns of genetic variations among and within cichlids in North-central Nigeria. This study, therefore, investigated population genetic structure of Coptodon zillii, Oreochromis niloticus, and Hemichromis fasciatus collected from different freshwater bodies in North-central Nigeria. Genomic DNA was extracted, and five highly polymorphic RAPD primers were used for RAPD-PCR amplification and genotyping of the fish. Genetic polymorphism within and between the three tilapia species were examined. Percentages of polymorphism loci, pairwise population matrix, analysis of molecular variance (AMOVA), and genetic distances of cichlid populations were determined using standard methods, and dendrograms were constructed using an un-weighted pair group method of arithmetic mean (UPGMA). Overall, percentages of estimated molecular variance within and among C. zillii, H. fasciatus and O. niloticus populations were 5% and 95%; 4% and 96% and 13% and 87%, respectively. Our results suggest that the three cichlids have close evolutionary relationship and there were no distinct genetic differences on the basis on sampling locations. C. zillii and H. fasciatus are more genetically closer than O. niloticus. This study concludes that RAPD is useful in studying the population genetic structure of cichlids. This study therefore recommends conservation of genetic pool of cichlid species through proper maintenance and restoration of polluted habitat to guarantee sustainable fishery production. However, markers such as microsatellite DNA can be assayed in further studies for better results.
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| first_indexed | 2024-04-12T12:33:33Z |
| format | Article |
| id | doaj.art-dc9cef6d20e3425790ef2e025cda6de3 |
| institution | Directory Open Access Journal |
| issn | 1609-5693 2410-7409 |
| language | English |
| last_indexed | 2024-04-12T12:33:33Z |
| publishDate | 2022-06-01 |
| publisher | University of Baghdad, College of Veterinary Medicine |
| record_format | Article |
| series | The Iraqi Journal of Veterinary Medicine |
| spelling | doaj.art-dc9cef6d20e3425790ef2e025cda6de32022-12-22T03:32:58ZengUniversity of Baghdad, College of Veterinary MedicineThe Iraqi Journal of Veterinary Medicine1609-56932410-74092022-06-0146110.30539/ijvm.v46i1.1307Population Genetic Structure of Three Cichlids in Ilorin, North-Central NigeriaOluyinka A Iyiola0Rahmat D Shaibu1Segun O Oladipo2Kazeem O Kareem3Lotanna M Nneji4Adeniyi C Adeola5Abass T Anifowoshe6Moise M Matouke7Cell Biology and Genetics Unit, Department of Zoology, University of Ilorin, P.M.B 1515, Ilorin Kwara state, Nigeria, Zoology Unit, Department of Bioscience and Biotechnology, Kwara State University, Malete, P.M.B 1530, Ilorin, Kwara state, NigeriaFaculty of Renewable Natural Resources, Department of Aquaculture and Fisheries Management, University of Ibadan, Ibadan, Nigeria, Faculty of Renewable Natural Resources, Department of Aquaculture and Fisheries Management, University of Ibadan, Ibadan, Nigeria, Department of Ecology and Environmental Biology, Princeton University, Princeton, New Jersey, United States of AmericaState Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China, Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, Bengaluru 560012, Karnataka, India, Institute of Science, Bangalore, Bengaluru 560012, Karnataka, India, 7Department of Fisheries and Aquatic Resources Management, University of Buea, Cameroon Cichlids are among the economically important which serve as a source of food for people around the world. A deep understanding of the population structure and genetic diversity of cichlids are vital for initiation of conservation policies and sustainable aquaculture. There is paucity of information on the patterns of genetic variations among and within cichlids in North-central Nigeria. This study, therefore, investigated population genetic structure of Coptodon zillii, Oreochromis niloticus, and Hemichromis fasciatus collected from different freshwater bodies in North-central Nigeria. Genomic DNA was extracted, and five highly polymorphic RAPD primers were used for RAPD-PCR amplification and genotyping of the fish. Genetic polymorphism within and between the three tilapia species were examined. Percentages of polymorphism loci, pairwise population matrix, analysis of molecular variance (AMOVA), and genetic distances of cichlid populations were determined using standard methods, and dendrograms were constructed using an un-weighted pair group method of arithmetic mean (UPGMA). Overall, percentages of estimated molecular variance within and among C. zillii, H. fasciatus and O. niloticus populations were 5% and 95%; 4% and 96% and 13% and 87%, respectively. Our results suggest that the three cichlids have close evolutionary relationship and there were no distinct genetic differences on the basis on sampling locations. C. zillii and H. fasciatus are more genetically closer than O. niloticus. This study concludes that RAPD is useful in studying the population genetic structure of cichlids. This study therefore recommends conservation of genetic pool of cichlid species through proper maintenance and restoration of polluted habitat to guarantee sustainable fishery production. However, markers such as microsatellite DNA can be assayed in further studies for better results. https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1307tilapia, random amplified polymorphic DNA markersgenetic diversityPCRNorthcentralfreshwater |
| spellingShingle | Oluyinka A Iyiola Rahmat D Shaibu Segun O Oladipo Kazeem O Kareem Lotanna M Nneji Adeniyi C Adeola Abass T Anifowoshe Moise M Matouke Population Genetic Structure of Three Cichlids in Ilorin, North-Central Nigeria The Iraqi Journal of Veterinary Medicine tilapia , random amplified polymorphic DNA markers genetic diversity PCR Northcentral freshwater |
| title | Population Genetic Structure of Three Cichlids in Ilorin, North-Central Nigeria |
| title_full | Population Genetic Structure of Three Cichlids in Ilorin, North-Central Nigeria |
| title_fullStr | Population Genetic Structure of Three Cichlids in Ilorin, North-Central Nigeria |
| title_full_unstemmed | Population Genetic Structure of Three Cichlids in Ilorin, North-Central Nigeria |
| title_short | Population Genetic Structure of Three Cichlids in Ilorin, North-Central Nigeria |
| title_sort | population genetic structure of three cichlids in ilorin north central nigeria |
| topic | tilapia , random amplified polymorphic DNA markers genetic diversity PCR Northcentral freshwater |
| url | https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1307 |
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