Applications of single-cell RNA sequencing in atopic dermatitis and psoriasis
Single-cell RNA sequencing (scRNA-seq) is a novel technology that characterizes molecular heterogeneity at the single-cell level. With the development of more automated, sensitive, and cost-effective single-cell isolation methods, the sensitivity and efficiency of scRNA-seq have improved. Technologi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1038744/full |
| _version_ | 1798016227872866304 |
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| author | Dengmei Xia Dengmei Xia Dengmei Xia Yiyi Wang Yiyi Wang Yue Xiao Yue Xiao Wei Li Wei Li |
| author_facet | Dengmei Xia Dengmei Xia Dengmei Xia Yiyi Wang Yiyi Wang Yue Xiao Yue Xiao Wei Li Wei Li |
| author_sort | Dengmei Xia |
| collection | DOAJ |
| description | Single-cell RNA sequencing (scRNA-seq) is a novel technology that characterizes molecular heterogeneity at the single-cell level. With the development of more automated, sensitive, and cost-effective single-cell isolation methods, the sensitivity and efficiency of scRNA-seq have improved. Technological advances in single-cell analysis provide a deeper understanding of the biological diversity of cells present in tissues, including inflamed skin. New subsets of cells have been discovered among common inflammatory skin diseases, such as atopic dermatitis (AD) and psoriasis. ScRNA-seq technology has also been used to analyze immune cell distribution and cell-cell communication, shedding new light on the complex interplay of components involved in disease responses. Moreover, scRNA-seq may be a promising tool in precision medicine because of its ability to define cell subsets with potential treatment targets and to characterize cell-specific responses to drugs or other stimuli. In this review, we briefly summarize the progress in the development of scRNA-seq technologies and discuss the latest scRNA-seq-related findings and future trends in AD and psoriasis. We also discuss the limitations and technical problems associated with current scRNA-seq technology. |
| first_indexed | 2024-04-11T15:47:20Z |
| format | Article |
| id | doaj.art-dc9ed98414e34c32af2537f05a095840 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T15:47:20Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-dc9ed98414e34c32af2537f05a0958402022-12-22T04:15:30ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-11-011310.3389/fimmu.2022.10387441038744Applications of single-cell RNA sequencing in atopic dermatitis and psoriasisDengmei Xia0Dengmei Xia1Dengmei Xia2Yiyi Wang3Yiyi Wang4Yue Xiao5Yue Xiao6Wei Li7Wei Li8Department of Dermatology, Rare Diseases Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Dermatology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, ChinaInstitutes for Systems Genetics, Frontiers Science Center for Disease-related Molecular Network, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Dermatology, Rare Diseases Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaInstitutes for Systems Genetics, Frontiers Science Center for Disease-related Molecular Network, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Dermatology, Rare Diseases Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaInstitutes for Systems Genetics, Frontiers Science Center for Disease-related Molecular Network, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Dermatology, Rare Diseases Center, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaInstitutes for Systems Genetics, Frontiers Science Center for Disease-related Molecular Network, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaSingle-cell RNA sequencing (scRNA-seq) is a novel technology that characterizes molecular heterogeneity at the single-cell level. With the development of more automated, sensitive, and cost-effective single-cell isolation methods, the sensitivity and efficiency of scRNA-seq have improved. Technological advances in single-cell analysis provide a deeper understanding of the biological diversity of cells present in tissues, including inflamed skin. New subsets of cells have been discovered among common inflammatory skin diseases, such as atopic dermatitis (AD) and psoriasis. ScRNA-seq technology has also been used to analyze immune cell distribution and cell-cell communication, shedding new light on the complex interplay of components involved in disease responses. Moreover, scRNA-seq may be a promising tool in precision medicine because of its ability to define cell subsets with potential treatment targets and to characterize cell-specific responses to drugs or other stimuli. In this review, we briefly summarize the progress in the development of scRNA-seq technologies and discuss the latest scRNA-seq-related findings and future trends in AD and psoriasis. We also discuss the limitations and technical problems associated with current scRNA-seq technology.https://www.frontiersin.org/articles/10.3389/fimmu.2022.1038744/fullsingle-cell RNA sequencinginflammationatopic dermatitispsoriasistranscriptomics |
| spellingShingle | Dengmei Xia Dengmei Xia Dengmei Xia Yiyi Wang Yiyi Wang Yue Xiao Yue Xiao Wei Li Wei Li Applications of single-cell RNA sequencing in atopic dermatitis and psoriasis Frontiers in Immunology single-cell RNA sequencing inflammation atopic dermatitis psoriasis transcriptomics |
| title | Applications of single-cell RNA sequencing in atopic dermatitis and psoriasis |
| title_full | Applications of single-cell RNA sequencing in atopic dermatitis and psoriasis |
| title_fullStr | Applications of single-cell RNA sequencing in atopic dermatitis and psoriasis |
| title_full_unstemmed | Applications of single-cell RNA sequencing in atopic dermatitis and psoriasis |
| title_short | Applications of single-cell RNA sequencing in atopic dermatitis and psoriasis |
| title_sort | applications of single cell rna sequencing in atopic dermatitis and psoriasis |
| topic | single-cell RNA sequencing inflammation atopic dermatitis psoriasis transcriptomics |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1038744/full |
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