The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes
Abstract Long non-coding RNAs (lncRNAs) play a vital role in regulating adipogenesis. However, the associated regulatory mechanisms have yet to be described in detail in pig. In this study, we demonstrate a critical role for lncMYOZ2 in adipogenesis from porcine preadipocytes. Specifically, lncMYOZ2...
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-022-08923-9 |
| _version_ | 1811238686453399552 |
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| author | Yang Yang Yiqi Wu Mengting Ji Xiaoyin Rong Yanwei Zhang Shuai Yang Chang Lu Chunbo Cai Pengfei Gao Xiaohong Guo Bugao Li Guoqing Cao |
| author_facet | Yang Yang Yiqi Wu Mengting Ji Xiaoyin Rong Yanwei Zhang Shuai Yang Chang Lu Chunbo Cai Pengfei Gao Xiaohong Guo Bugao Li Guoqing Cao |
| author_sort | Yang Yang |
| collection | DOAJ |
| description | Abstract Long non-coding RNAs (lncRNAs) play a vital role in regulating adipogenesis. However, the associated regulatory mechanisms have yet to be described in detail in pig. In this study, we demonstrate a critical role for lncMYOZ2 in adipogenesis from porcine preadipocytes. Specifically, lncMYOZ2 was more abundant in the adipose tissue of Mashen (fat-type) pigs than for Large White (lean-type) pigs, and knockdown of this lncRNA significantly inhibited the differentiation of porcine preadipocytes into adipocytes. Mechanistically, we used RNA pull-down and RIP assays to establish that lncMYOZ2 interacts with adenosylhomocysteinase (AHCY). Moreover, lncMYOZ2 knockdown increased promoter methylation of the target gene MYOZ2 and lowered its expression. Finally, we describe a positive regulatory role for MYOZ2 in adipogenesis. Collectively, these findings establish lncMYOZ2 as an important epigenetic regulator of adipogenesis via the aforementioned AHCY/MYOZ2 pathway, and provide insights into the role of lncRNAs in porcine adipose development. |
| first_indexed | 2024-04-12T12:46:34Z |
| format | Article |
| id | doaj.art-dca0bac1b0144396a7d430fe39f36df6 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T12:46:34Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-dca0bac1b0144396a7d430fe39f36df62022-12-22T03:32:36ZengBMCBMC Genomics1471-21642022-10-012311910.1186/s12864-022-08923-9The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytesYang Yang0Yiqi Wu1Mengting Ji2Xiaoyin Rong3Yanwei Zhang4Shuai Yang5Chang Lu6Chunbo Cai7Pengfei Gao8Xiaohong Guo9Bugao Li10Guoqing Cao11College of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityAbstract Long non-coding RNAs (lncRNAs) play a vital role in regulating adipogenesis. However, the associated regulatory mechanisms have yet to be described in detail in pig. In this study, we demonstrate a critical role for lncMYOZ2 in adipogenesis from porcine preadipocytes. Specifically, lncMYOZ2 was more abundant in the adipose tissue of Mashen (fat-type) pigs than for Large White (lean-type) pigs, and knockdown of this lncRNA significantly inhibited the differentiation of porcine preadipocytes into adipocytes. Mechanistically, we used RNA pull-down and RIP assays to establish that lncMYOZ2 interacts with adenosylhomocysteinase (AHCY). Moreover, lncMYOZ2 knockdown increased promoter methylation of the target gene MYOZ2 and lowered its expression. Finally, we describe a positive regulatory role for MYOZ2 in adipogenesis. Collectively, these findings establish lncMYOZ2 as an important epigenetic regulator of adipogenesis via the aforementioned AHCY/MYOZ2 pathway, and provide insights into the role of lncRNAs in porcine adipose development.https://doi.org/10.1186/s12864-022-08923-9lncRNAlncMYOZ2Porcine preadipocytesAdipogenesisAHCYMYOZ2 |
| spellingShingle | Yang Yang Yiqi Wu Mengting Ji Xiaoyin Rong Yanwei Zhang Shuai Yang Chang Lu Chunbo Cai Pengfei Gao Xiaohong Guo Bugao Li Guoqing Cao The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes BMC Genomics lncRNA lncMYOZ2 Porcine preadipocytes Adipogenesis AHCY MYOZ2 |
| title | The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes |
| title_full | The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes |
| title_fullStr | The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes |
| title_full_unstemmed | The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes |
| title_short | The long non-coding RNA lncMYOZ2 mediates an AHCY/MYOZ2 axis to promote adipogenic differentiation in porcine preadipocytes |
| title_sort | long non coding rna lncmyoz2 mediates an ahcy myoz2 axis to promote adipogenic differentiation in porcine preadipocytes |
| topic | lncRNA lncMYOZ2 Porcine preadipocytes Adipogenesis AHCY MYOZ2 |
| url | https://doi.org/10.1186/s12864-022-08923-9 |
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